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Acetyl-CoA Carboxylase Alpha Gene Polymorphism and Its Association with Milk Fatty Acid of Holstein Friesian Using Real-Time PCR Method

Tropical Animal Science Journal · Vol. 43 No. 4 (2020) · hlm. 306–313

DOI
10.5398/tasj.2020.43.4.306
ISSN
2615-790X, 2615-787X
Terbit
2020-12-01
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Milk fatty acids are largely affected by genetic factors. Acetyl-CoA Carboxylase Alpha (ACACA) gene is one of the important genes in regulation and metabolic function of milk fatty acids in dairy cattle. The objective of this study was to find out the relationship of single nucleotide polymorphism (SNP) ACACA gene with milk fatty acid trait in local dairy cattle. A total of 277 samples of Holstein Friesian (HF) were collected from Indonesian Research Institute for Animal Production (IRIAP), Animal Breeding Center and Forage Feed of Baturraden (ABCFFB), Central Java Province, Animal Husbandry Training Center of Cikole (AHTCC), West Java Province, Singosari Artificial Insemination Station (Singosari AIS), East Java Province, and Lembang Artificial Insemination (Lembang AIS), West Java Province, Indonesia. Genotyping of this SNP marker (g.2203G>T) was analyzed using the real-time Polymerase Chain Reaction (PCR) based on the hybridization TaqMan probe as the method for allelic discrimination. Milk samples were analyzed using Gas Chromatography and Mass Spectrometry (GCMS). The results of this study revealed the GG and GT genotypes. The proportion of the GG genotype frequency (0.88) was higher than the GT genotype (0.11) and the G Allele frequency was shown higher than the T allele in all locations, i.e., 0.942 and 0.08, respectively. The ACACA gene g.2203G>T SNP was significant (p T SNP could be useful as a marker selection for milk fatty acid such as lauric and dodecanoic fatty acids.

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  • IPB University
  • National Research and Innovation Agency
  • Universitas Sumatera Utara
  • Universitas Nahdlatul Ulama Blitar
  • Universitas Halu Oleo
  • Indonesian Agency for Agricultural Instruments Standardization
  • University of Brawijaya
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Rekan penulisOrganisasiArtikel bersama
Cece SumantriIPB University20
Ratna Sholatia HarahapIPB University5
Kasita ListyariniIPB University5
Jakaria JakariaIPB University4
Achmad FarajallahIPB University3
Ferdy SaputraNational Research and Innovation Agency3
Niken UlupiIPB University3
Chalid TalibIPB University3
Anneke AnggraeniNational Research and Innovation Agency2
Eryk AndreasIPB University2
Ismeth InounuIPB University2
Sri MurtiniIPB University2
Ronny Rachman NoorIPB University2
S. MurtiniIPB University2
Rudy PriyantoIPB University2
. MuladnoAfiliasi tidak diketahui2
. AryogiAfiliasi tidak diketahui2
. HartatiAfiliasi tidak diketahui2
Muh. RusdinUniversitas Halu Oleo2
Andi Baso Lompengeng IshakIndonesian Agency for Agricultural Instruments Standardization2
Salman YusufAfiliasi tidak diketahui1
Sri RahayuUniversity of Brawijaya1
Adinda Wanda AstiraAfiliasi tidak diketahui1
Apri IriantoIPB University1
Muladno MuladnoIPB University1
Mutasem Ali M. AbuzahraAfiliasi tidak diketahui1
Dewi Apri AstutiIPB University1
Elmy MarianaIPB University1
Muhammad Ary SyaputraUniversitas Sumatera Utara1
Sayed UmarUniversitas Sumatera Utara1
Nafisa Aliya PutriAfiliasi tidak diketahui1
Harry Safary MargapradjaAfiliasi tidak diketahui1
Rohmat DiyonoAfiliasi tidak diketahui1
Nury HsAfiliasi tidak diketahui1
D PerwitasariAfiliasi tidak diketahui1
Fisma AmriIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Acetyl-CoA Carboxylase Alpha Gene Polymorphism and Its Association with Milk Fatty Acid of Holstein Friesian Using Real-Time PCR Method</dc:title>
	<dc:creator xml:lang="en">Azis, R.</dc:creator>
	<dc:creator xml:lang="en">Jakaria</dc:creator>
	<dc:creator xml:lang="en">Anggraeni, A.</dc:creator>
	<dc:creator xml:lang="en">Gunawan, A.</dc:creator>
	<dc:subject xml:lang="en">eal-time PCR</dc:subject>
	<dc:subject xml:lang="en">TaqMan MGB probe</dc:subject>
	<dc:subject xml:lang="en">genotype</dc:subject>
	<dc:subject xml:lang="en">ACACA gene</dc:subject>
	<dc:subject xml:lang="en">Holstein Friesian</dc:subject>
	<dc:description xml:lang="en">Milk fatty acids are largely affected by genetic factors. Acetyl-CoA Carboxylase Alpha (ACACA) gene is one of the important genes in regulation and metabolic function of milk fatty acids in dairy cattle. The objective of this study was to find out the relationship of single nucleotide polymorphism (SNP) ACACA gene with milk fatty acid trait in local dairy cattle. A total of 277 samples of Holstein Friesian (HF) were collected from Indonesian Research Institute for Animal Production (IRIAP), Animal Breeding Center and Forage Feed of Baturraden (ABCFFB), Central Java Province, Animal Husbandry Training Center of Cikole (AHTCC), West Java Province, Singosari Artificial Insemination Station (Singosari AIS), East Java Province, and Lembang Artificial Insemination (Lembang AIS), West Java Province, Indonesia. Genotyping of this SNP marker (g.2203G&amp;gt;T) was analyzed using the real-time Polymerase Chain Reaction (PCR) based on the hybridization TaqMan probe as the method for allelic discrimination. Milk samples were analyzed using Gas Chromatography and Mass Spectrometry (GCMS). The results of this study revealed the GG and GT genotypes. The proportion of the GG genotype frequency (0.88) was higher than the GT genotype (0.11) and the G Allele frequency was shown higher than the T allele in all locations, i.e., 0.942 and 0.08, respectively. The ACACA gene g.2203G&amp;gt;T SNP was significant (p&amp;lt;0.05) for lauric (C12:0) and dodecanoic (C17:1) acids. It was concluded that the ACACA gene g.2203G&amp;gt;T SNP could be useful as a marker selection for milk fatty acid such as lauric and dodecanoic fatty acids.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2020-12-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/30075</dc:identifier>
	<dc:identifier>10.5398/tasj.2020.43.4.306</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 43 No. 4 (2020): Tropical Animal Science Journal; 306-313</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2020.43.4</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/30075/20781</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>