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Detection of Class 1 Integron Encoding Gene in Multidrug Resistance (MDR) Citrobacter freundii Isolated from Healthy Broiler Chicken

Tropical Animal Science Journal · Vol. 44 No. 3 (2021) · hlm. 363–368

DOI
10.5398/tasj.2021.44.3.363
ISSN
2615-790X, 2615-787X
Terbit
2021-08-19
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

This study was aimed to find out that broiler chicken farms have problems with antibiotic resistance Citrobacter freundii and determined the prevalence and class 1 integron encoding gene. Multidrug resistance Citrobacter freundii was collected from broiler chicken among one hundred and sixty cloacal swab samples from 32 farms in Blitar for 3 months. The method of bacterial inoculation used MacConkey agar and biochemical test was conducted by IMViC and TSIA test. Citrobacter freundii for antibiotic sensitivity pattern was tested by disk diffusion, and the multidrug resistance encoding gene was tested by PCR. This study exposed 160 samples, and 13.75% (22/160) samples were positive of Citrobacter freundii. The antibiotic sensitivity pattern showed high resistances against ampicillin and erythromycin (77.27%), tetracycline (59.09%), trimetropim-sulfamethoxazole (50%), and streptomycin (22.72%). Isolates that were detected as multidrug resistance were continued with PCR testing to prove the existence of a class 1 integron encoding gene. Multidrug resistance Citrobacter freundii isolated from broiler chicken farms in Blitar were 81.82% (18/22), and were indicated that five were positive Class 1 Integron encoding gene. The results of this study showed that the prevalence and distribution of multidrug resistance Citrobacter freundii were high, so it can cause the spread of antimicrobial resistance to public health. Class 1 integron encoding gene was found 22.72% from multidrug resistance Citrobacter freundii by PCR. It was concluded that broiler chicken farms need assessment management to reduce and avoid multidrug resistance bacteria in animals and human. Therefore, the use of appropriate antibiotics is a good step to reduce the incidence of MDR in poultry.

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  • Universitas Airlangga
  • Universitas Negeri Surabaya
  • Universitas Wijaya Kusuma Surabaya
  • National Research and Innovation Agency
  • Universitas Perjuangan Tasikmalaya
  • Yamaguchi University
  • Gomal University
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Rekan penulisOrganisasiArtikel bersama
Aswin Rafif KhairullahNational Research and Innovation Agency7
MARIANA FEBRILIANTI RESILINDA PUTRIUniversitas Airlangga4
Sheila Marty YanestriaUniversitas Wijaya Kusuma Surabaya4
Prima Ayu WibawatiUniversitas Airlangga3
Nenny HarijaniUniversitas Airlangga3
Saifur RehmanGomal University3
Imam MustofaUniversitas Airlangga2
Mutasem AbuzahraUniversitas Airlangga2
Dwi WijayantiUniversitas Perjuangan Tasikmalaya2
Loay Abu EidYamaguchi University2
Sri ChusniatiUniversitas Airlangga2
Sancaka Chasyer RamandiniantoUniversitas Airlangga2
Hani PlumeriastutiUniversitas Airlangga2
Abdullah HasibThe University of Queensland2
Sin War NawMyitkyina University1
Shifa FauziyahUniversitas Airlangga1
Teguh Hari SuciptoUniversitas Airlangga1
Mirwa Adiprahara AnggaraniUniversitas Negeri Surabaya1
Dzikra Nasyaya MahfudhahUniversitas Negeri Surabaya1
Fistara Lesti RahmafitriaUniversitas Negeri Surabaya1
Nuniek HerdyastutiUniversitas Negeri Surabaya1
Aditya YudhanaUniversitas Airlangga1
Adhitya Yhoga PradikaUniversitas Airlangga1
Adelita Putri SevitasariUniversitas Airlangga1
Maya Nurwartanti YunitaUniversitas Airlangga1
Safira Fauziyyah SubagyoUniversitas Airlangga1
Lucia Tri SuwantiUniversitas Airlangga1
Jola RahmahaniUniversitas Airlangga1
Wiwiek TyasningsihUniversitas Airlangga1
Shinta Levea Ni'matul FadlilahUniversitas Airlangga1
Dhandy Koesoemo WardhanaUniversitas Airlangga1
Devi Ayu SafitriUniversitas Airlangga1
Suwaibatul AnnisaUniversitas Airlangga1
Yolla Rona MustikaUniversitas Airlangga1
Yulianna PuspitasariUniversitas Airlangga1
Kurnia Nisa KinasihUniversitas Airlangga1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Detection of Class 1 Integron Encoding Gene in Multidrug Resistance (MDR) Citrobacter freundii Isolated from Healthy Broiler Chicken</dc:title>
	<dc:creator xml:lang="en">Witaningrum, A. M.</dc:creator>
	<dc:creator xml:lang="en">Wibisono, F. J.</dc:creator>
	<dc:creator xml:lang="en"> Permatasari, D. A.</dc:creator>
	<dc:creator xml:lang="en">Effendi, M. H.</dc:creator>
	<dc:subject xml:lang="en">broiler chicken</dc:subject>
	<dc:subject xml:lang="en">Citrobacter freundii</dc:subject>
	<dc:subject xml:lang="en">Class 1 integron encoding gene</dc:subject>
	<dc:subject xml:lang="en">multidrug resistance</dc:subject>
	<dc:subject xml:lang="en">public health</dc:subject>
	<dc:description xml:lang="en">This study was aimed to find out that broiler chicken farms have problems with antibiotic resistance Citrobacter freundii and determined the prevalence and class 1 integron encoding gene. Multidrug resistance Citrobacter freundii was collected from broiler chicken among one hundred and sixty cloacal swab samples from 32 farms in Blitar for 3 months. The method of bacterial inoculation used MacConkey agar and biochemical test was conducted by IMViC and TSIA test. Citrobacter freundii for antibiotic sensitivity pattern was tested by disk diffusion, and the multidrug resistance encoding gene was tested by PCR. This study exposed 160 samples, and 13.75% (22/160) samples were positive of Citrobacter freundii. The antibiotic sensitivity pattern showed high resistances against ampicillin and erythromycin (77.27%), tetracycline (59.09%), trimetropim-sulfamethoxazole (50%), and streptomycin (22.72%). Isolates that were detected as multidrug resistance were continued with PCR testing to prove the existence of a class 1 integron encoding gene. Multidrug resistance Citrobacter freundii isolated from broiler chicken farms in Blitar were 81.82% (18/22), and were indicated that five were positive Class 1 Integron encoding gene. The results of this study showed that the prevalence and distribution of multidrug resistance Citrobacter freundii were high, so it can cause the spread of antimicrobial resistance to public health. Class 1 integron encoding gene was found 22.72% from multidrug resistance Citrobacter freundii by PCR. It was concluded that broiler chicken farms need assessment management to reduce and avoid multidrug resistance bacteria in animals and human. Therefore, the use of appropriate antibiotics is a good step to reduce the incidence of MDR in poultry.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2021-08-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/32368</dc:identifier>
	<dc:identifier>10.5398/tasj.2021.44.3.363</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 44 No. 3 (2021): Tropical Animal Science Journal; 363-368</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2021.44.3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/32368/22108</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2021 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>