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Improved Maturation Rate of Bovine Oocytes Following Sericin Supplementation in Collection and Maturation Media

Tropical Animal Science Journal · Vol. 45 No. 1 (2022) · hlm. 24–29

DOI
10.5398/tasj.2022.45.1.24
ISSN
2615-790X, 2615-787X
Terbit
2022-02-04
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Sericin is a water-soluble protein produced by silk cocoons and known to have antioxidant activity. This study is aimed to analyze the nuclear maturation and the quality of bovine oocytes in the collection and in vitro maturation (IVM) medium supplemented with sericin. Bovine oocytes were collected using a collection medium supplemented with sericin in 0 (control) concentrations, 0.1%, 0.5%, and 1%. Selected oocytes were then matured for 24 h at 38.5 oC in 5% CO₂ and evaluated for nuclear maturation. In the subsequent experiment, oocytes were collected and matured with or without 0.1% sericin at 38.5 oC in 5% CO2. Matured oocytes were counterstained with terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) and Hoechst 33342. Matured oocytes were characterized by oocytes that reached the MII stage. The results showed that supplementation of 0.1% sericin in the collection medium increased the number of oocytes reaching the metaphase II (MII) stage compared to the control group (p<0.05). In the next experiment, sericin 0.1% in the collection and in vitro maturation media increased (p<0.05) the percentage of oocytes reaching the MII stage compared to control without sericin supplementation. Furthermore, the number of fragmented DNA in the oocytes showed no differences in all groups. It can be concluded that supplementation of 0.1% sericin in the collection and in vitro maturation media improved the nuclear status without affecting DNA fragmentation.

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  • IPB University
  • National Research and Innovation Agency
  • Mississippi State University
  • Lambung Mangkurat University
  • Institute of Agrobiological Sciences
  • Pusat Penelitian dan Pengembangan Peternakan
  • Padjadjaran University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
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  • Afiliasi tidak diketahui
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Raden Iis ArifiantiniIPB University16
Iman SupriatnaIPB University8
Tulus MaulanaNational Research and Innovation Agency6
M. GunawanNational Research and Innovation Agency6
Mokhamad FahrudinIPB University5
Polmer Zaelinga SitumorangAfiliasi tidak diketahui5
Wahono Esthi PrasetyaningtyasIPB University5
Syahruddin SaidNational Research and Innovation Agency5
AmroziAfiliasi tidak diketahui4
Mozes R. ToelihereAfiliasi tidak diketahui4
Muhammad AgilIPB University4
Naseer A. KutchyMississippi State University4
Dondin SajuthiIPB University3
Tuty L YusufIPB University3
T.L YusufAfiliasi tidak diketahui3
Muhammad RizalLambung Mangkurat University3
Kazuhiro KikuchiInstitute of Agrobiological Sciences2
Mohamad Agus SetiadiIPB University2
Dedy Duryadi SolihinIPB University2
I Ketut SutamaAfiliasi tidak diketahui2
Riasari Gail SianturiAfiliasi tidak diketahui2
YudiIPB University2
Kusuma DiwyantoPusat Penelitian dan Pengembangan Peternakan2
Jack J RutledgeAfiliasi tidak diketahui2
HERDISAfiliasi tidak diketahui2
Arief BoedionoIPB University2
Tatan KostamanNational Research and Innovation Agency2
Aisyah Fidela SiregarIPB University1
Anis AuliaAfiliasi tidak diketahui1
Ghinna Aulia RasyidaAfiliasi tidak diketahui1
Fathul BariAfiliasi tidak diketahui1
Septiyani SeptiyaniPadjadjaran University1
Cece SumantriIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Improved Maturation Rate of Bovine Oocytes Following Sericin Supplementation in Collection and Maturation Media</dc:title>
	<dc:creator xml:lang="en">Satrio, F. A.</dc:creator>
	<dc:creator xml:lang="en">Karja, N. W. K.</dc:creator>
	<dc:creator xml:lang="en">Setiadi, M. A. </dc:creator>
	<dc:creator xml:lang="en">Kaiin, E. M. </dc:creator>
	<dc:creator xml:lang="en">Gunawan, M. </dc:creator>
	<dc:creator xml:lang="en">Memili, E. </dc:creator>
	<dc:creator xml:lang="en">Purwantara, B. </dc:creator>
	<dc:subject xml:lang="en">bovine oocyte</dc:subject>
	<dc:subject xml:lang="en">collection medium</dc:subject>
	<dc:subject xml:lang="en">in vitro</dc:subject>
	<dc:subject xml:lang="en">maturation</dc:subject>
	<dc:subject xml:lang="en">sericin</dc:subject>
	<dc:description xml:lang="en">Sericin is a water-soluble protein produced by silk cocoons and known to have antioxidant activity. This study is aimed to analyze the nuclear maturation and the quality of bovine oocytes in the collection and in vitro maturation (IVM) medium supplemented with sericin. Bovine oocytes were collected using a collection medium supplemented with sericin in 0 (control) concentrations, 0.1%, 0.5%, and 1%. Selected oocytes were then matured for 24 h at 38.5 oC in 5% CO₂ and evaluated for nuclear maturation. In the subsequent experiment, oocytes were collected and matured with or without 0.1% sericin at 38.5 oC in 5% CO2. Matured oocytes were counterstained with terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) and Hoechst 33342. Matured oocytes were characterized by oocytes that reached the MII stage. The results showed that supplementation of 0.1% sericin in the collection medium increased the number of oocytes reaching the metaphase II (MII) stage compared to the control group (p&amp;lt;0.05). In the next experiment, sericin 0.1% in the collection and in vitro maturation media increased (p&amp;lt;0.05) the percentage of oocytes reaching the MII stage compared to control without sericin supplementation. Furthermore, the number of fragmented DNA in the oocytes showed no differences in all groups. It can be concluded that supplementation of 0.1% sericin in the collection and in vitro maturation media improved the nuclear status without affecting DNA fragmentation.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2022-02-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/34684</dc:identifier>
	<dc:identifier>10.5398/tasj.2022.45.1.24</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 45 No. 1 (2022): Tropical Animal Science Journal; 24-29</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2022.45.1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/34684/22957</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>