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Variation and Association of Avian β-Defensin 2 Gene with the Concentration of Immunoglobulin Y and the Titer of Newcastle-Disease Antibody in IPB-D1 Chicken

Tropical Animal Science Journal · Vol. 45 No. 2 (2022) · hlm. 121–127

DOI
10.5398/tasj.2022.45.2.121
ISSN
2615-790X, 2615-787X
Terbit
2022-05-18
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Defensins play roles in innate immunity by exhibiting antimicrobial activity against microbes such as gram-negative and -positive bacteria, viruses, and fungi. This study aimed to identify variants of the Avian β-Defensin 2 (AvBD2) and determine their associations with the concentration of immunoglobulin Y and the titer of Newcastle disease (ND) antibody in IPB-1 chicken. The chicken population used in this study was 21-week-old IPB-D1 chickens (n=90). Variations in AvBD2 were analyzed by direct DNA sequencing. IgY concentration was measured by indirect ELISA, and the titer of ND antibody was measured by the hemagglutination-inhibition test. The AvBD2 association was analyzed by the general linear model procedure and Duncan’s multiple range test. The results revealed 10 SNPs located in intron 1 (3 SNPs), exon 2 (3 SNPs), and intron 2 (4 SNPs). Six of these SNPs were associated with IgY concentration. The CC genotype of g.5002 C>T was associated with IgY concentration and produced the highest mean IgY concentration. This g.5002 C>T mutation results in alanine-to-valine substitutions. The CC genotype of g.5002 C>T could be considered as a criterion for selecting chickens with high IgY concentrations.

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  • IPB University
  • National Research and Innovation Agency
  • Padjadjaran University
  • Universitas Djuanda
  • Indonesian Agency for Agricultural Instruments Standardization
  • Universitas Halu Oleo
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  • Organisasi berbeda
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Rekan penulisOrganisasiArtikel bersama
Sri MurtiniIPB University14
Jakaria JakariaIPB University13
Ronny Rachman NoorIPB University12
Kasita ListyariniIPB University10
Ratna Sholatia HarahapIPB University9
Anneke AnggraeniNational Research and Innovation Agency8
A. AnggraeniAfiliasi tidak diketahui7
Ismeth InounuIPB University6
Achmad FarajallahIPB University5
Raden Iis ArifiantiniIPB University5
Eryk AndreasIPB University4
Iman SupriatnaIPB University4
SubandriyoUniversitas Djuanda4
Eko HandiwirawanNational Research and Innovation Agency4
Irma Isnafia AriefIPB University4
Dedy Duryadi SolihinIPB University4
Arief BoedionoIPB University3
Andi Baso Lompengeng IshakIndonesian Agency for Agricultural Instruments Standardization3
Ferdy SaputraNational Research and Innovation Agency3
Dewi Apri AstutiIPB University3
Sri DarwatiIPB University3
Cahyo BudimanIPB University3
Saiful AnwarNational Research and Innovation Agency3
Hera MaheshwariIPB University3
Muhammad Amrullah PagalaUniversitas Halu Oleo2
Hera MaheshwariAfiliasi tidak diketahui2
Aryani Sismin SatyaningtijasAfiliasi tidak diketahui2
Siti Darodjah RasadPadjadjaran University2
Nena HilmiaPadjadjaran University2
Noer Muhammad Dliyaul HaqIPB University2
Diah Nugrahani PristihadiIPB University2
Rudy PriyantoIPB University2
Widya Pintaka Bayu PutraNational Research and Innovation Agency2

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Variation and Association of Avian β-Defensin 2 Gene with the Concentration of Immunoglobulin Y and the Titer of Newcastle-Disease Antibody in  IPB-D1 Chicken</dc:title>
	<dc:creator xml:lang="en">Lestari, D.</dc:creator>
	<dc:creator xml:lang="en">Masruroh</dc:creator>
	<dc:creator xml:lang="en">Khaerunnisa, I.</dc:creator>
	<dc:creator xml:lang="en">Murtini, S.</dc:creator>
	<dc:creator xml:lang="en">Ulupi, N.</dc:creator>
	<dc:creator xml:lang="en">Gunawan, A.</dc:creator>
	<dc:creator xml:lang="en">Sumantri, C.</dc:creator>
	<dc:subject xml:lang="en">AvBD2</dc:subject>
	<dc:subject xml:lang="en">IPB-D1 chicken</dc:subject>
	<dc:subject xml:lang="en">IgY concentration</dc:subject>
	<dc:subject xml:lang="en">Newcastle disease antibody titer</dc:subject>
	<dc:description xml:lang="en">Defensins play roles in innate immunity by exhibiting antimicrobial activity against microbes such as gram-negative and -positive bacteria, viruses, and fungi. This study aimed to identify variants of the Avian β-Defensin 2 (AvBD2) and determine their associations with the concentration of immunoglobulin Y and the titer of Newcastle disease (ND) antibody in IPB-1 chicken. The chicken population used in this study was 21-week-old IPB-D1 chickens (n=90). Variations in AvBD2 were analyzed by direct DNA sequencing. IgY concentration was measured by indirect ELISA, and the titer of ND antibody was measured by the hemagglutination-inhibition test. The AvBD2 association was analyzed by the general linear model procedure and Duncan’s multiple range test. The results revealed 10 SNPs located in intron 1 (3 SNPs), exon 2 (3 SNPs), and intron 2 (4 SNPs). Six of these SNPs were associated with IgY concentration. The CC genotype of g.5002 C&amp;gt;T was associated with IgY concentration and produced the highest mean IgY concentration. This g.5002 C&amp;gt;T mutation results in alanine-to-valine substitutions. The CC genotype of g.5002 C&amp;gt;T could be considered as a criterion for selecting chickens with high IgY concentrations.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2022-05-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/35878</dc:identifier>
	<dc:identifier>10.5398/tasj.2022.45.2.121</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 45 No. 2 (2022): Tropical Animal Science Journal; 121-127</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/35878/23422</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>