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The Viabilities of Freeze-Thaw Pasundan-Bull Sperms After a Short-Term Exposure to Media with Different pHs

Tropical Animal Science Journal · Vol. 45 No. 3 (2022) · hlm. 270–276

DOI
10.5398/tasj.2022.45.3.270
ISSN
2615-790X, 2615-787X
Terbit
2022-08-16
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

External pH is crucial in preserving sperm viability and ensuring fertilization during in vitro conditions. The purpose of this study was to determine the maximum pH value that can be tolerated by frozen-thawed Pasundan bull sperms and the effect on sperm quality. Around 250x106 sperms/mL of frozen-thawed Pasundan bull sperms were divided into ten equal aliquots, and each was diluted in the medium within a particular pH value. HCL or NaOH was added to the buffer media to create ten different solutions with varying pH values of 3, 4, 5, 6 as acidic, 7.2-7.4 as a control, and 8, 9, 10, 11, and 12 as alkaline. Furthermore, the samples were incubated for 5 minutes at room temperature within a particular pH medium before being immediately supplemented with a buffered medium to achieve a pH of 7.2-7.4. After 10 minutes of incubation at room temperature, all parameters were assessed. The results showed that sperm motility, viability, normal morphology, and acrosome intactness in sperms incubated in the acidic or alkaline media were significantly lower compared to control (p<0.05, respectively). Interestingly, the sperm still had a good tolerance to pHs 6 and 8. This tolerance was evidenced by all the parameters of sperms that were not sharply decreased compared to the control group. The significant loss of motility occurred at pHs 3 and 12. It could be concluded that frozen-thawed Pasundan bull sperms are still tolerable in pHs 4-11, but the sperm quality degrades as the acidity or alkaline level increases.acidic

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  • IPB University
  • National Research and Innovation Agency
  • Padjadjaran University
  • Lambung Mangkurat University
  • Universitas Djuanda
  • Indonesian Agency for Agricultural Instruments Standardization
  • Indonesian Institute of Sciences
  • Afiliasi tidak diketahui
  • Organisasi berbeda
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Asep GunawanIPB University14
Ronny Rachman NoorIPB University10
Muhammad RizalLambung Mangkurat University10
Jakaria JakariaIPB University9
HERDISAfiliasi tidak diketahui7
Sri MurtiniIPB University6
Anneke AnggraeniNational Research and Innovation Agency6
Isyana KhaerunnisaNational Research and Innovation Agency6
A. AnggraeniAfiliasi tidak diketahui6
Niken UlupiIPB University6
Mokhamad FahrudinIPB University6
Kasita ListyariniIPB University5
Achmad FarajallahIPB University5
Iman SupriatnaIPB University5
. YulnawatiAfiliasi tidak diketahui5
Ratna Sholatia HarahapIPB University4
Eryk AndreasIPB University4
Ismeth InounuIPB University4
SubandriyoUniversitas Djuanda4
Eko HandiwirawanNational Research and Innovation Agency4
Yulnawati YulnawatiIndonesian Institute of Sciences4
Berry JuliandiIPB University3
Irma Isnafia AriefIPB University3
Andi Baso Lompengeng IshakIndonesian Agency for Agricultural Instruments Standardization3
Dedy Duryadi SolihinIPB University3
Ferdy SaputraNational Research and Innovation Agency3
Raden Iis ArifiantiniIPB University3
Wasmen ManaluIPB University3
Dewi Apri AstutiIPB University2
Sri DarwatiIPB University2
Siti Darodjah RasadPadjadjaran University2
Nena HilmiaPadjadjaran University2
Bambang PurwantaraIPB University2

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">The Viabilities of Freeze-Thaw Pasundan-Bull Sperms After a Short-Term Exposure to Media with Different pHs</dc:title>
	<dc:creator xml:lang="en">Widyastuti, R.</dc:creator>
	<dc:creator xml:lang="en">Haq, N. M. D.</dc:creator>
	<dc:creator xml:lang="en">Pristihadi, D. N.</dc:creator>
	<dc:creator xml:lang="en">Wahyudin</dc:creator>
	<dc:creator xml:lang="en">Maheshwari, H.</dc:creator>
	<dc:creator xml:lang="en">Sumantri, C.</dc:creator>
	<dc:creator xml:lang="en">Boediono, A.</dc:creator>
	<dc:subject xml:lang="en">acidic</dc:subject>
	<dc:subject xml:lang="en">alkaline</dc:subject>
	<dc:subject xml:lang="en">Pasundan bull</dc:subject>
	<dc:subject xml:lang="en">sperm</dc:subject>
	<dc:description xml:lang="en">External pH is crucial in preserving sperm viability and ensuring fertilization during in vitro conditions. The purpose of this study was to determine the maximum pH value that can be tolerated by frozen-thawed Pasundan bull sperms and the effect on sperm quality. Around 250x106 sperms/mL of frozen-thawed Pasundan bull sperms were divided into ten equal aliquots, and each was diluted in the medium within a particular pH value. HCL or NaOH was added to the buffer media to create ten different solutions with varying pH values of 3, 4, 5, 6 as acidic, 7.2-7.4 as a control, and 8, 9, 10, 11, and 12 as alkaline. Furthermore, the samples were incubated for 5 minutes at room temperature within a particular pH medium before being immediately supplemented with a buffered medium to achieve a pH of 7.2-7.4. After 10 minutes of incubation at room temperature, all parameters were assessed. The results showed that sperm motility, viability, normal morphology, and acrosome intactness in sperms incubated in the acidic or alkaline media were significantly lower compared to control (p&amp;lt;0.05, respectively). Interestingly, the sperm still had a good tolerance to pHs 6 and 8. This tolerance was evidenced by all the parameters of sperms that were not sharply decreased compared to the control group. The significant loss of motility occurred at pHs 3 and 12. It could be concluded that frozen-thawed Pasundan bull sperms are still tolerable in pHs 4-11, but the sperm quality degrades as the acidity or alkaline level increases.acidic</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2022-08-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/36978</dc:identifier>
	<dc:identifier>10.5398/tasj.2022.45.3.270</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 45 No. 3 (2022): Tropical Animal Science Journal; 270-276</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2022.45.3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/36978/23948</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>