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The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents

Tropical Animal Science Journal · Vol. 45 No. 3 (2022) · hlm. 284–290

DOI
10.5398/tasj.2022.45.3.284
ISSN
2615-790X, 2615-787X
Terbit
2022-08-16
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Diluents are substances added to the semen to increase the semen volume, reduce sperm density, and maintain sperm viability. This study aimed to compare the effects of various semen diluents on the quality of frozen semen of Limousin bull. Three mature Limousin bulls were used as semen sources. The semen was collected using an artificial vagina and then evaluated for its quality. Sperms with motility less than 70% and abnormality greater than 20% were excluded from this study. After semen evaluation, each ejaculate was individually divided into four equal tubes and diluted with skimmed milk-egg yolk (SMEY), Tris–egg yolk (TEY), Tris–egg yolk–skimmed milk (TEYSM), or Andromed®. Diluted semen was loaded into a mini straw and then equilibrated at 5 °C for 4 h. Following equilibration, the straws were frozen. The percentages of sperm motility, viability, intact membrane, and sperm DNA integrity were evaluated after freezing. Furthermore, malondialdehyde (MDA) and aspartate aminotransferase (AST) enzyme concentrations were assessed after thawing. The sperm motility with TEY, TEYSM, and Andromed® did not differ significantly but was higher than with SMEY. Sperm viability, membrane intactness, and sperm DNA integrity did not differ among treatments. By contrast, the MDA levels and concentration of AST in the Andromed® diluent semen showed the lowest values compared with SMEY semen (p<0.05) but did not differ from those of TEY and TEYSM semen. To conclude, TEY, TEYSM, and Andromed® diluents performed equally well, and although they showed better results than SMEY, sperm diluted in SMEY are still suitable for artificial insemination.

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  • IPB University
  • National Research and Innovation Agency
  • Hasanuddin University
  • Universitas Gadjah Mada
  • Mississippi State University
  • Lambung Mangkurat University
  • Pusat Penelitian dan Pengembangan Peternakan
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Ni Wayan Kurniani KarjaIPB University13
Syahruddin SaidNational Research and Innovation Agency10
Iman SupriatnaIPB University7
Mohamad Agus SetiadiIPB University7
Muhammad GunawanNational Research and Innovation Agency6
Polmer Zaelinga SitumorangAfiliasi tidak diketahui5
Faisal Amri SatrioIPB University5
Cece SumantriIPB University5
AmroziAfiliasi tidak diketahui4
Mozes R. ToelihereAfiliasi tidak diketahui4
Wahono Esthi PrasetyaningtyasIPB University4
Aeni NurlatifahUniversitas Gadjah Mada4
Herdis HerdisNational Research and Innovation Agency4
T.L YusufAfiliasi tidak diketahui4
I Ketut SutamaAfiliasi tidak diketahui4
Hikmayani IskandarHasanuddin University4
M. GunawanNational Research and Innovation Agency4
Muhammad AgilIPB University3
Dondin SajuthiIPB University3
Lilis KhotijahIPB University3
Tuty L YusufIPB University3
Erdoğan MemiliMississippi State University3
YudiIPB University3
A. GunawanIPB University3
Dedy Duryadi SolihinIPB University2
Riasari Gail SianturiAfiliasi tidak diketahui2
Muhammad RizalLambung Mangkurat University2
Hasbi HasbiHasanuddin University2
Herry SonjayaHasanuddin University2
Kusuma DiwyantoPusat Penelitian dan Pengembangan Peternakan2
Jack J RutledgeAfiliasi tidak diketahui2
Dewi Apri AstutiIPB University2
HERDISAfiliasi tidak diketahui2
RENE H IERAfiliasi tidak diketahui1
Mette H. SchmidtAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents</dc:title>
	<dc:creator xml:lang="en">Arif, A. A.             </dc:creator>
	<dc:creator xml:lang="en"> Maulana, T.</dc:creator>
	<dc:creator xml:lang="en"> Kaiin, E. M.</dc:creator>
	<dc:creator xml:lang="en">Purwantara, B.</dc:creator>
	<dc:creator xml:lang="en">Arifiantini, R. I.</dc:creator>
	<dc:subject xml:lang="en">AST</dc:subject>
	<dc:subject xml:lang="en">frozen semen of Limousin</dc:subject>
	<dc:subject xml:lang="en">MDA</dc:subject>
	<dc:subject xml:lang="en">semen diluent</dc:subject>
	<dc:description xml:lang="en">Diluents are substances added to the semen to increase the semen volume, reduce sperm density, and maintain sperm viability. This study aimed to compare the effects of various semen diluents on the quality of frozen semen of Limousin bull. Three mature Limousin bulls were used as semen sources. The semen was collected using an artificial vagina and then evaluated for its quality. Sperms with motility less than 70% and abnormality greater than 20% were excluded from this study. After semen evaluation, each ejaculate was individually divided into four equal tubes and diluted with skimmed milk-egg yolk (SMEY), Tris–egg yolk (TEY), Tris–egg yolk–skimmed milk (TEYSM), or Andromed®. Diluted semen was loaded into a mini straw and then equilibrated at 5 °C for 4&amp;nbsp;h. Following equilibration, the straws were frozen. The percentages of sperm motility, viability, intact membrane, and sperm DNA integrity were evaluated after freezing. Furthermore, malondialdehyde (MDA) and aspartate aminotransferase (AST) enzyme concentrations were assessed after thawing. The sperm motility with TEY, TEYSM, and Andromed® did not differ significantly but was higher than with SMEY. Sperm viability, membrane intactness, and sperm DNA integrity did not differ among treatments. By contrast, the MDA levels and concentration of AST in the Andromed® diluent semen showed the lowest values compared with SMEY semen (p&amp;lt;0.05) but did not differ from those of TEY and TEYSM semen. To conclude, TEY, TEYSM, and Andromed® diluents performed equally well, and although they showed better results than SMEY, sperm diluted in SMEY are still suitable for artificial insemination.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2022-08-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/36996</dc:identifier>
	<dc:identifier>10.5398/tasj.2022.45.3.284</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 45 No. 3 (2022): Tropical Animal Science Journal; 284-290</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2022.45.3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/36996/23950</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>