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Detection of Plasma Membrane Alpha Enolase (ENO1) and Its Relationship with Sperm Quality of Bali Cattle

Tropical Animal Science Journal · Vol. 46 No. 1 (2023) · hlm. 36–42

DOI
10.5398/tasj.2023.46.1.36
ISSN
2615-790X, 2615-787X
Terbit
2023-03-03
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Bali cattle are the indigenous cattle well known attributed to their high fertility. Various proteomes in sperm are substances that can determine sperm quality and fertility. One of which is alpha enolase (ENO1). This study aims to assess the presence of ENO1 in the plasma membrane of sperm and its relationship with the semen quality of Bali cattle. The study used 30 ejaculates from 5 bulls for sperm quality assessment and detection of ENO1. Sperm quality is indicated by total motility, progressive motility, kinematics motility, viability, plasma membrane integrity, and ENO1 quantity. Sperm motility and kinematics motility were measured using CASA, while viability was assessed by eosin-nigrosine differential staining. The HOS test was used to determine plasma membrane integrity, and ENO1 quantity was measured by the ELISA method. Data were analyzed using ANOVA (randomized group design), linear regression, Pearson’s correction, and the t-Student test. The results showed that ENO1 was detected in the plasma membrane at 1.27 ng/106 sperms. The amount of alpha-enolase in the plasma membrane of Bali cattle sperm is affected by sperm concentration (p<0.01) and not involved in sperm motility. There was no correlation between plasma membrane ENO1 quantity and semen quality. The results of this experiment indicated that alpha-enolase in the plasma membrane of Bali cattle sperm is affected by sperm concentration but not related to sperm quality.

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  • Universitas Halu Oleo
  • IPB University
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Rekan penulisOrganisasiArtikel bersama
T.L YusufAfiliasi tidak diketahui7
AmroziAfiliasi tidak diketahui7
P SitumorangAfiliasi tidak diketahui6
N. W. K. KarjaAfiliasi tidak diketahui6
E.M KaiinAfiliasi tidak diketahui6
R.I ArifiantiniAfiliasi tidak diketahui5
M.R. ToelihereAfiliasi tidak diketahui4
Riasari Gail SianturiAfiliasi tidak diketahui4
F. A. SatrioAfiliasi tidak diketahui4
M. GunawanAfiliasi tidak diketahui4
A BoedionoAfiliasi tidak diketahui4
C. SumantriAfiliasi tidak diketahui3
R.R. NoorAfiliasi tidak diketahui3
D. SajuthiAfiliasi tidak diketahui3
YudiAfiliasi tidak diketahui3
I-K SutamaAfiliasi tidak diketahui2
Muhammad RizalUniversitas Halu Oleo2
I ArifiantiniAfiliasi tidak diketahui2
E.D GunardiAfiliasi tidak diketahui2
K DiwyantoAfiliasi tidak diketahui2
J.J RutledgeAfiliasi tidak diketahui2
HERDISAfiliasi tidak diketahui2
I.G PutuAfiliasi tidak diketahui2
S SaidAfiliasi tidak diketahui2
. YulnawatiAfiliasi tidak diketahui1
M ImronAfiliasi tidak diketahui1
W. Marlene Mesang-NalleyAfiliasi tidak diketahui1
R HandariniAfiliasi tidak diketahui1
N.M ArtiningsihiAfiliasi tidak diketahui1
R.K AchjadiAfiliasi tidak diketahui1
Bambang Ngaji UtomoAfiliasi tidak diketahui1
E.D. GurnadiAfiliasi tidak diketahui1
E.T. SetiatinAfiliasi tidak diketahui1
Cece SumantriIPB University1
D D SolihinAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Detection of Plasma Membrane Alpha Enolase (ENO1) and Its Relationship with Sperm Quality of Bali Cattle</dc:title>
	<dc:creator xml:lang="en">Sumarsono, T.</dc:creator>
	<dc:creator xml:lang="en">Supriatna, I.</dc:creator>
	<dc:creator xml:lang="en">Setiadi, M. A.</dc:creator>
	<dc:creator xml:lang="en">Agil, M.</dc:creator>
	<dc:creator xml:lang="en">Purwantara, B.</dc:creator>
	<dc:subject xml:lang="en">ENO1</dc:subject>
	<dc:subject xml:lang="en">plasma membrane</dc:subject>
	<dc:subject xml:lang="en">proteomes</dc:subject>
	<dc:subject xml:lang="en">sperm quality</dc:subject>
	<dc:description xml:lang="en">Bali cattle are the indigenous cattle well known attributed to their high fertility. Various proteomes in sperm are substances that can determine sperm quality and fertility. One of which is alpha enolase (ENO1). This study aims to assess the presence of ENO1 in the plasma membrane of sperm and its relationship with the semen quality of Bali cattle. The study used 30 ejaculates from 5 bulls for sperm quality assessment and detection of ENO1. Sperm quality is indicated by total motility, progressive motility, kinematics motility, viability, plasma membrane integrity, and ENO1 quantity. Sperm motility and kinematics motility were measured using CASA, while viability was assessed by eosin-nigrosine differential staining. The HOS test was used to determine plasma membrane integrity, and ENO1 quantity was measured by the ELISA method. Data were analyzed using ANOVA (randomized group design), linear regression, Pearson’s correction, and the t-Student test. The results showed that ENO1 was detected in the plasma membrane at 1.27 ng/106 sperms. The amount of alpha-enolase in the plasma membrane of Bali cattle sperm is affected by sperm concentration (p&amp;lt;0.01) and not involved in sperm motility. There was no correlation between plasma membrane ENO1 quantity and semen quality. The results of this experiment indicated that alpha-enolase in the plasma membrane of Bali cattle sperm is affected by sperm concentration but not related to sperm quality.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2023-03-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/42082</dc:identifier>
	<dc:identifier>10.5398/tasj.2023.46.1.36</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 46 No. 1 (2023): Tropical Animal Science Journal; 36-42</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2023.46.1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/42082/24994</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>