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Methicillin Resistant Staphylococcus aureus (MRSA) Isolation and mecA Gene Detection from Milk and Farmer Hand Swab in Tulungagung, Indonesia

Tropical Animal Science Journal · Vol. 46 No. 2 (2023) · hlm. 231–238

DOI
10.5398/tasj.2023.46.2.231
ISSN
2615-790X, 2615-787X
Terbit
2023-05-24
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Staphylococcus aureus is a harmful bacterium that often contaminates milk; hence it is believed to become a severe health risk for humans. S. aureus resistant to β‑lactam drugs can be termed methicillin resistant Staphylococcus aureus (MRSA). Dairy farms have a high incidence of MRSA infections due to the repeated use of the same medicines on dairy cows and the physical contact between farmers and cows during milking. This study looked for MRSA in dairy cow milk and farmer hand swabs in Tulungagung, Indonesia. Using oxacillin and cefoxitin diffusion disks, phenotypic detection approaches were evaluated, then transferred to the Oxacillin Resistance Screening Agar Base (ORSAB) test and genotypically verified using PCR to find the mecA gene encoding MRSA. One hundred ten dairy cow milk samples and 45 farmer's hand swabs were collected from Tulungagung, East Java, Indonesia. Mannitol salt agar (MSA) was used for cultivation and purification. The disk-diffusion test used oxacillin and cefoxitin to identify S. aureus resistance. Oxacillin and cefoxitin-resistant S. aureus isolates were tested for MRSA using ORSAB. In addition, MRSA isolates were PCR-tested for the mecA gene. S. aureus was found in 110 (70.97%) of 155 isolates. Of the total 110 isolates of S. aureus, 16 (14.54%) and 39 (35.45%) were known to be resistant to Cefoxitin and Oxacillin, respectively. When tested with ORSAB, 23 isolates from 55 resistant isolates showed positive results for MRSA. Dairy milk was the source of most MRSA which is 15 isolates, while hand swabs only carried 8 isolates. However, PCR analysis only found mecA gene in two isolates. According to this study, many MRSA isolates were found from dairy farms in Tulungagung, Indonesia, but only a few have the mecA gene.

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  • Universitas Airlangga
  • National Research and Innovation Agency
  • Universitas Wijaya Kusuma Surabaya
  • Wageningen University & Research
  • University of Indonesia
  • Binus University
  • Indonesian Agency for Agricultural Research and Development
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Rekan penulisOrganisasiArtikel bersama
Siti Rani AyutiUniversitas Syiah Kuala7
Abdullah HasibThe University of Queensland7
Mirni LamidUniversitas Airlangga7
Ikechukwu Benjamin MosesEbonyi State University6
Freshinta Jellia WibisonoUniversitas Wijaya Kusuma Surabaya6
Sheila Marty YanestriaUniversitas Wijaya Kusuma Surabaya6
Lucia Tri SuwantiUniversitas Airlangga6
Widya Paramita LokapirnasariUniversitas Airlangga6
Nenny HarijaniUniversitas Airlangga5
Riza Zainuddin AhmadNational Research and Innovation Agency5
Sri ChusniatiUniversitas Airlangga4
Jola RahmahaniUniversitas Airlangga4
Yulianna PuspitasariUniversitas Airlangga4
Prima Ayu WibawatiUniversitas Airlangga3
Imam MustofaUniversitas Airlangga3
Mutasem AbuzahraUniversitas Airlangga3
Hani PlumeriastutiUniversitas Airlangga3
Thomas Valentinus WidiyatnoUniversitas Airlangga3
Ira Sari YudaniayantiUniversitas Airlangga3
Mufasirin MufasirinUniversitas Airlangga3
Endang SuprihatiUniversitas Airlangga3
Dwi WijayantiUniversitas Perjuangan Tasikmalaya2
Loay Abu EidYamaguchi University2
Fitrine EkawastiNational Research and Innovation Agency2
Setiawan KoesdartoUniversitas Airlangga2
Ratna DamayantiUniversitas Airlangga2
Mohammad Anam Al ArifUniversitas Airlangga2
Sunaryo Hadi WarsitoUniversitas Airlangga2
Tita Damayanti LestariUniversitas Airlangga2

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Methicillin Resistant Staphylococcus aureus (MRSA) Isolation and mecA Gene Detection from Milk and Farmer Hand Swab in Tulungagung, Indonesia</dc:title>
	<dc:creator xml:lang="en">Khairullah, A. R.</dc:creator>
	<dc:creator xml:lang="en">Kurniawan, S. C.</dc:creator>
	<dc:creator xml:lang="en">Silaen, O. S. M.</dc:creator>
	<dc:creator xml:lang="en">Effendi, M. H.</dc:creator>
	<dc:creator xml:lang="en">Sudjarwo, S. A.</dc:creator>
	<dc:creator xml:lang="en">Ramandinianto, S. C.</dc:creator>
	<dc:creator xml:lang="en">Gololodo, M. A.</dc:creator>
	<dc:creator xml:lang="en">Widodo, A.</dc:creator>
	<dc:creator xml:lang="en">Riwu, K. H. P.</dc:creator>
	<dc:creator xml:lang="en">Kurniawati, D. A.</dc:creator>
	<dc:creator xml:lang="en">Rehman, S.</dc:creator>
	<dc:subject xml:lang="en">milk</dc:subject>
	<dc:subject xml:lang="en">swab hand</dc:subject>
	<dc:subject xml:lang="en">mecA</dc:subject>
	<dc:subject xml:lang="en">MRSA</dc:subject>
	<dc:subject xml:lang="en">public health</dc:subject>
	<dc:description xml:lang="en">Staphylococcus aureus&amp;nbsp;is a harmful bacterium that often contaminates milk; hence it is believed to become a severe health risk for humans. S. aureus&amp;nbsp;resistant to β‑lactam drugs can be termed methicillin resistant&amp;nbsp;Staphylococcus aureus&amp;nbsp;(MRSA). Dairy farms have a high incidence of MRSA infections due to the repeated use of the same medicines on dairy cows and the physical contact between farmers and cows during milking. This study looked for MRSA in dairy cow milk and farmer hand swabs in Tulungagung, Indonesia. Using oxacillin and cefoxitin diffusion disks, phenotypic detection approaches were evaluated, then transferred to the Oxacillin Resistance Screening Agar Base (ORSAB) test and genotypically verified using PCR to find the mecA gene encoding MRSA. One hundred ten dairy cow milk samples and 45 farmer's hand swabs were collected from Tulungagung, East Java, Indonesia. Mannitol salt agar (MSA) was used for cultivation and purification. The disk-diffusion test used oxacillin and cefoxitin to identify&amp;nbsp;S.&amp;nbsp;aureus&amp;nbsp;resistance. Oxacillin and cefoxitin-resistant&amp;nbsp;S. aureus&amp;nbsp;isolates were tested for MRSA using ORSAB. In addition, MRSA isolates were PCR-tested for the mecA gene.&amp;nbsp;S. aureus&amp;nbsp;was found in 110 (70.97%) of 155 isolates. Of the total 110 isolates of&amp;nbsp;S. aureus, 16 (14.54%) and 39 (35.45%) were known to be resistant to Cefoxitin and Oxacillin, respectively. When tested with ORSAB, 23 isolates from 55 resistant isolates showed positive results for MRSA. Dairy milk was the source of most MRSA which is 15 isolates, while hand swabs only carried 8 isolates. However, PCR analysis only found mecA gene in two isolates. According to this study, many MRSA isolates were found from dairy farms in Tulungagung, Indonesia, but only a few have the mecA gene.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2023-05-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/42978</dc:identifier>
	<dc:identifier>10.5398/tasj.2023.46.2.231</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 46 No. 2 (2023): Tropical Animal Science Journal; 231-238</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2023.46.2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/42978/25594</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>