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Optimization of Phytase Production from Rhodotorula mucilaginosa RG-PK20 Using Agricultural Waste

Tropical Animal Science Journal · Vol. 48 No. 4 (2025) · hlm. 328–337

DOI
10.5398/tasj.2025.48.4.328
ISSN
2615-790X, 2615-787X
Terbit
2025-06-30
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng

Abstrak

Some ingredients in poultry feed contain phytic acid, which prevents the absorption of nutrients. Microbial phytase enzymes can help with this problem. However, the phytase-producing gene of the novel yeast Rhodotorula mucilaginosa RG-PK20 has been constrained by the high cost of traditional substrates such as sodium phytate. The aim of this study is to evaluate the production of phytase from R. mucilaginosa RG-PK20 using a phytic acid source (substrate) from agricultural waste, with in vitro testing in poultry feed. The fermentation process was conducted utilizing a substrate-to-medium-to-yeast culture ratio of 1:1:1 v/v/w over a period of four days at various temperatures (25, 28, and 30 °C) and pH levels (3, 4, 5, and 6, with 7 as a control). Glucose and urea supplements were given when the optimal conditions were established by measuring the phytase content and activity. The molecular weight of the phytase was confirmed by SDS-PAGE analysis, and the ability of the enzyme to hydrolyze phytic acid was evaluated in vitro. Corn cobs generated the highest amount of phytase, with a concentration of 25.29 mg/mL and activity of 4.46 U/mL. The in vitro tests revealed an 81% reduction in phytic acid levels in poultry feed. These results demonstrate the potential of phytase derived from R. mucilaginosa RG-PK20 to reduce phytic acid in poultry feed ingredients.

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  • Universitas Esa Unggul
  • Riau University
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Rekan penulisOrganisasiArtikel bersama
Wahyu LestariRiau University1
Annisa KhaeraniUniversitas Esa Unggul1
Laras SitoayuUniversitas Esa Unggul1
Nazhif GifariUniversitas Esa Unggul1
Rachmanida NuzrinaUniversitas Esa Unggul1
Harna HarnaUniversitas Esa Unggul1
Dudung AngkasaUniversitas Esa Unggul1
Dian RahayuningsihUniversitas Esa Unggul1
Nadiyah NadiyahUniversitas Esa Unggul1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Optimization of Phytase Production from Rhodotorula mucilaginosa RG-PK20 Using Agricultural Waste</dc:title>
	<dc:creator xml:lang="en">Seprianto</dc:creator>
	<dc:creator xml:lang="en">Utama, C. C. Y.</dc:creator>
	<dc:creator xml:lang="en">Melani, V.</dc:creator>
	<dc:creator xml:lang="en">Handayani, P.</dc:creator>
	<dc:creator xml:lang="en">Sukarman</dc:creator>
	<dc:subject xml:lang="en">agricultural waste</dc:subject>
	<dc:subject xml:lang="en">phytase</dc:subject>
	<dc:subject xml:lang="en">phytic acid</dc:subject>
	<dc:subject xml:lang="en">poultry feed</dc:subject>
	<dc:subject xml:lang="en">Rhodotorula mucilaginosa</dc:subject>
	<dc:description xml:lang="en">Some ingredients in poultry feed contain phytic acid, which prevents the absorption of nutrients. Microbial phytase enzymes can help with this problem. However, the phytase-producing gene of the novel yeast Rhodotorula mucilaginosa RG-PK20 has been constrained by the high cost of traditional substrates such as sodium phytate. The aim of this study is to evaluate the production of phytase from R. mucilaginosa RG-PK20 using a phytic acid source (substrate) from agricultural waste, with in vitro testing in poultry feed. The fermentation process was conducted utilizing a substrate-to-medium-to-yeast culture ratio of 1:1:1 v/v/w over a period of four days at various temperatures (25, 28, and 30 °C) and pH levels (3, 4, 5, and 6, with 7 as a control). Glucose and urea supplements were given when the optimal conditions were established by measuring the phytase content and activity. The molecular weight of the phytase was confirmed by SDS-PAGE analysis, and the ability of the enzyme to hydrolyze phytic acid was evaluated in vitro. Corn cobs generated the highest amount of phytase, with a concentration of 25.29 mg/mL and activity of 4.46 U/mL. The in vitro tests revealed an 81% reduction in phytic acid levels in poultry feed. These results demonstrate the potential of phytase derived from R. mucilaginosa RG-PK20 to reduce phytic acid in poultry feed ingredients.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2025-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/61596</dc:identifier>
	<dc:identifier>10.5398/tasj.2025.48.4.328</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 48 No. 4 (2025): Tropical Animal Science Journal; 328-337</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2025.48.4</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/61596/31124</dc:relation>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/61596/34322</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Tropical Animal Science Journal</dc:rights>
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