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Proteomic Analysis of Pesisir Bull Sperm in Different Age Groups for the Identification of Reproductive Function Proteins

Tropical Animal Science Journal · Vol. 48 No. 5 (2025) · hlm. 393–401

DOI
10.5398/tasj.2025.48.5.393
ISSN
2615-790X, 2615-787X
Terbit
2025-08-27
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Pesisir cattle are native to Indonesia and originate from West Sumatra. It is known for its high environmental adaptability and can be further developed using a proteomic approach. The proteomic approach for testing the fertility of Pesisir bulls is an important factor in determining a potentially superior breed. Proteomic studies of reproduction in Pesisir bulls have not been widely conducted. This study aimed to identify and characterize functional sperm proteins in Pesisir bulls that are associated with reproductive processes. Semen samples were collected from 12 pesisir bulls aged 2–4 years and grouped into three age categories. Protein profile analysis was performed using polyacrylamide gel electrophoresis with 1D-SDS-PAGE, followed by proteolytic digestion of the proteins in the gel and protein identification using LC-MS/MS analysis. Protein functions were predicted based on analysis of biological annotations generated using UniProt, Venny, PANTHER, and STRING. Based on the evaluation of fresh semen, sperm motility in Pesisir bulls was found to increase with age. Proteomic analysis successfully identified 334 proteins in the sperm of Pesisir bulls. Among these, two proteins, ZPBP and SPACA3, were identified as involved in reproductive and fertilisation processes. Functionally, both proteins play crucial roles in acrosomal events during fertilisation. Gene Ontology analysis showed that most proteins in sperm are involved in various biological processes, including cellular activities, metabolic processes, and molecular functions related to catalytic activities. In conclusion, SPACA3 and ZPBP proteins were identified as potentially involved in reproductive processes and may serve as fertility markers in Pesisir bulls.

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  • IPB University
  • National Research and Innovation Agency
  • Universitas Gadjah Mada
  • Universitas Djuanda
  • Indonesian Agency for Agricultural Instruments Standardization
  • Hasanuddin University
  • Padjadjaran University
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  • Organisasi berbeda
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Asep GunawanIPB University15
Ronny Rachman NoorIPB University10
Jakaria JakariaIPB University10
Bambang PurwantaraIPB University8
Ni Wayan Kurniani KarjaIPB University7
Syahruddin SaidNational Research and Innovation Agency7
Sri MurtiniIPB University6
Anneke AnggraeniNational Research and Innovation Agency6
Isyana KhaerunnisaNational Research and Innovation Agency6
A. AnggraeniAfiliasi tidak diketahui6
Kasita ListyariniIPB University6
Niken UlupiIPB University6
Achmad FarajallahIPB University5
Ismeth InounuIPB University5
Iman SupriatnaIPB University5
Ratna Sholatia HarahapIPB University4
Eryk AndreasIPB University4
SubandriyoUniversitas Djuanda4
Eko HandiwirawanNational Research and Innovation Agency4
Aeni NurlatifahUniversitas Gadjah Mada4
Herdis HerdisNational Research and Innovation Agency4
Ekayanti Mulyawati KaiinNational Research and Innovation Agency4
Dewi Apri AstutiIPB University4
Arief BoedionoIPB University3
Irma Isnafia AriefIPB University3
Wahono Esthi PrasetyaningtyasIPB University3
Lilis KhotijahIPB University3
Andi Baso Lompengeng IshakIndonesian Agency for Agricultural Instruments Standardization3
Dedy Duryadi SolihinIPB University3
Ferdy SaputraNational Research and Innovation Agency3
Cahyo BudimanIPB University3
Hikmayani IskandarHasanuddin University3
Tike SartikaNational Research and Innovation Agency3
Sri DarwatiIPB University2
Siti Darodjah RasadPadjadjaran University2

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Proteomic Analysis of Pesisir Bull Sperm in Different Age Groups for the Identification of Reproductive Function Proteins</dc:title>
	<dc:creator xml:lang="en">Anwar, P.</dc:creator>
	<dc:creator xml:lang="en">Maulana, T. </dc:creator>
	<dc:creator xml:lang="en">Sumantri, C.</dc:creator>
	<dc:creator xml:lang="en">Arifiantini, I. </dc:creator>
	<dc:creator xml:lang="en">Gunawan, A. </dc:creator>
	<dc:subject xml:lang="en">Pesisir bulls</dc:subject>
	<dc:subject xml:lang="en">proteomic</dc:subject>
	<dc:subject xml:lang="en">markers</dc:subject>
	<dc:subject xml:lang="en">ZPBP</dc:subject>
	<dc:subject xml:lang="en">SPACA3</dc:subject>
	<dc:description xml:lang="en">Pesisir cattle are native to Indonesia and originate from West Sumatra. It is known for its high environmental adaptability and can be further developed using a proteomic approach. The proteomic approach for testing the fertility of Pesisir bulls is an important factor in determining a potentially superior breed. Proteomic studies of reproduction in Pesisir bulls have not been widely conducted. This study aimed to identify and characterize functional sperm proteins in Pesisir bulls that are associated with reproductive processes. Semen samples were collected from 12 pesisir bulls aged 2–4 years and grouped into three age categories. Protein profile analysis was performed using polyacrylamide gel electrophoresis with 1D-SDS-PAGE, followed by proteolytic digestion of the proteins in the gel and protein identification using LC-MS/MS analysis. Protein functions were predicted based on analysis of biological annotations generated using UniProt, Venny, PANTHER, and STRING. Based on the evaluation of fresh semen, sperm motility in Pesisir bulls was found to increase with age. Proteomic analysis successfully identified 334 proteins in the sperm of Pesisir bulls. Among these, two proteins, ZPBP and SPACA3, were identified as involved in reproductive and fertilisation processes. Functionally, both proteins play crucial roles in acrosomal events during fertilisation. Gene Ontology analysis showed that most proteins in sperm are involved in various biological processes, including cellular activities, metabolic processes, and molecular functions related to catalytic activities. In conclusion, SPACA3 and ZPBP proteins were identified as potentially involved in reproductive processes and may serve as fertility markers in Pesisir bulls.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2025-08-27</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/64344</dc:identifier>
	<dc:identifier>10.5398/tasj.2025.48.5.393</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 48 No. 5 (2025): Tropical Animal Science Journal; 393-401</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2025.48.5</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/64344/31372</dc:relation>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/64344/34308</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Tropical Animal Science Journal</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>