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In Vitro Anthelmintic Evaluation of Acanthophora spicifera Macroalgal Extract Against Haemonchus contortus in Sheep

Tropical Animal Science Journal · Vol. 48 No. 6 (2025) · hlm. 508–520

DOI
10.5398/tasj.2025.48.6.508
ISSN
2615-790X, 2615-787X
Terbit
2025-10-13
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

This study investigated the chemical composition and anthelmintic activity of Acanthophora spicifera against Haemonchus contortus from sheep in vitro. Fresh macroalgae were collected from Sepanjang Beach, Gunungkidul, Indonesia. Proximate analysis, fiber fractions, mineral composition, and color profile analysis were conducted to determine chemical profile of the macroalga. It was extracted by maceration with 96% ethanol (1:5) and ultrasonication. Secondary metabolites analyzed from the extract included total flavonoids, phenols, tannins, and saponins. The anthelmintic activity was tested in vitro through H. contortus adult worm motility and egg hatch inhibition tests. Extract treatments included P0: 0.9% physiological NaCl (negative control); P1: 0.5 mg/mL macroalgal extract; P2: 1 mg/mL macroalgal extract; P3: 1.5 mg/mL macroalgal extract; and Palb: 0.5 mg/mL albendazole (positive control). Motility test results showed that the use of macroalgae at levels of 0.5, 1, and 1.5 mg/mL significantly reduced H. contortus motility (p<0.05). However, effective LD₅₀ was reached in 1 and 1.5 mg/mL within the first 10 hours, and LD₁₀₀ after 21 hours (p<0.05). None of the extract levels matched the efficacy of albendazole (p<0.05). The egg hatch inhibition test revealed that the use of macroalgae at levels of 0.5, 1, and 1.5 mg/mL significantly inhibited H. contortus egg hatching by more than 85.73% after 24 hours (p<0.01), demonstrating comparable efficacy to albendazole. Based on these findings, A. spicifera extract contains various secondary metabolites with anthelmintic activity, inhibiting both H. contortus adult worm motility and egg hatching. This suggests that A. spicifera has potential for further development as a bioanthelmintic for ruminant parasite control, although further in vivo studies are needed.

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  • State University of Semarang
  • University of Indonesia
  • Cenderawasih University
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
H. JulendraAfiliasi tidak diketahui4
M. F. KarimyAfiliasi tidak diketahui3
Priyantini WidiyaningrumAfiliasi tidak diketahui2
A. JayanegaraAfiliasi tidak diketahui2
Abinawanto AbinawantoUniversity of Indonesia2
Yasman YasmanAfiliasi tidak diketahui2
T. HartatikAfiliasi tidak diketahui2
S. BintaraAfiliasi tidak diketahui2
L. IstiqomahAfiliasi tidak diketahui2
E. DamayantiAfiliasi tidak diketahui2
A. E. SuryaniAfiliasi tidak diketahui2
Kustantinah KustantinahAfiliasi tidak diketahui2
A. IrawanAfiliasi tidak diketahui2
S.Pd., M.Hum., Imam BaehaqieAfiliasi tidak diketahui1
Sri SukaesihState University of Semarang1
Andin IrsadiState University of Semarang1
Endah PeniatiState University of Semarang1
Ahmad SofyanCenderawasih University1
H. HerdianAfiliasi tidak diketahui1
E.B LaconiAfiliasi tidak diketahui1
R. S. H. MartinAfiliasi tidak diketahui1
Nahrowi .Afiliasi tidak diketahui1
R. P. HarahapAfiliasi tidak diketahui1
C. HidayatAfiliasi tidak diketahui1
M. M. SholikinAfiliasi tidak diketahui1
SadarmanAfiliasi tidak diketahui1
ElfawatiAfiliasi tidak diketahui1
R. K. RusliAfiliasi tidak diketahui1
R. SolfaineAfiliasi tidak diketahui1
N. A. HidayatiAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">In Vitro Anthelmintic Evaluation of Acanthophora spicifera Macroalgal Extract Against Haemonchus contortus in Sheep</dc:title>
	<dc:creator xml:lang="en">Sakti, A. A.</dc:creator>
	<dc:creator xml:lang="en">Kustantinah</dc:creator>
	<dc:creator xml:lang="en">Suwignyo, B.</dc:creator>
	<dc:creator xml:lang="en">Sofyan, A.</dc:creator>
	<dc:creator xml:lang="en">Panjono</dc:creator>
	<dc:creator xml:lang="en">Nurcahyo, R. W.</dc:creator>
	<dc:creator xml:lang="en">Baihaqi, Z. A.</dc:creator>
	<dc:creator xml:lang="en">Harahap, M. A.</dc:creator>
	<dc:creator xml:lang="en">Wulandari</dc:creator>
	<dc:creator xml:lang="en">Prasetyo</dc:creator>
	<dc:subject xml:lang="en">Acanthophora spicifera</dc:subject>
	<dc:subject xml:lang="en">anthelmintic</dc:subject>
	<dc:subject xml:lang="en">Haemonchus contortus</dc:subject>
	<dc:subject xml:lang="en">macroalgae</dc:subject>
	<dc:subject xml:lang="en">sheep</dc:subject>
	<dc:description xml:lang="en">This study investigated the chemical composition and anthelmintic activity of Acanthophora spicifera against Haemonchus contortus from sheep in vitro. Fresh macroalgae were collected from Sepanjang Beach, Gunungkidul, Indonesia. Proximate analysis, fiber fractions, mineral composition, and color profile analysis were conducted to determine chemical profile of the macroalga. It was extracted by maceration with 96% ethanol (1:5) and ultrasonication. Secondary metabolites analyzed from the extract included total flavonoids, phenols, tannins, and saponins. The anthelmintic activity was tested in vitro through H. contortus adult worm motility and egg hatch inhibition tests. Extract treatments included P0: 0.9% physiological NaCl (negative control); P1: 0.5 mg/mL macroalgal extract; P2: 1 mg/mL macroalgal extract; P3: 1.5 mg/mL macroalgal extract; and Palb: 0.5 mg/mL albendazole (positive control). Motility test results showed that the use of macroalgae at levels of 0.5, 1, and 1.5 mg/mL significantly reduced H. contortus motility (p&amp;lt;0.05). However, effective LD₅₀ was reached in 1 and 1.5 mg/mL within the first 10 hours, and LD₁₀₀ after 21 hours (p&amp;lt;0.05). None of the extract levels matched the efficacy of albendazole (p&amp;lt;0.05). The egg hatch inhibition test revealed that the use of macroalgae at levels of 0.5, 1, and 1.5 mg/mL significantly inhibited H. contortus egg hatching by more than 85.73% after 24 hours (p&amp;lt;0.01), demonstrating comparable efficacy to albendazole. Based on these findings, A. spicifera extract contains various secondary metabolites with anthelmintic activity, inhibiting both H. contortus adult worm motility and egg hatching. This suggests that A. spicifera has potential for further development as a bioanthelmintic for ruminant parasite control, although further in vivo studies are needed.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2025-10-13</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/65224</dc:identifier>
	<dc:identifier>10.5398/tasj.2025.48.6.508</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 48 No. 6 (2025): Tropical Animal Science Journal; 508-520</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2025.48.6</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/65224/31796</dc:relation>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/65224/34300</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Tropical Animal Science Journal</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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