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Comparative Analysis of Semen Quality and Kinematics in Sexed andNon-Sexed Sperm of Limousin Bulls

Tropical Animal Science Journal · Vol. 49 No. 2 (2026)

DOI
10.5398/tasj.2026.49.2.114
ISSN
2615-790X, 2615-787X
Terbit
2026-02-11
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The application of spermatozoa sexing technology offers benefits in determining the desired sex in cattle. However, this technology requires particular attention to quality, DNA fragmentation, and kinematics of spermatozoa. This study aimed to evaluate the effect of sexed and non-sexed semen on spermatozoa quality, DNA fragmentation, and kinematics in Limousin bulls. Four Limousin bulls between the ages of four and seven years and weighing between 610 and 814 kg were used in this study. The sexing method followed the bovine serum albumin (BSA) protocol. The upper fraction (5% BSA) was used for X-spermatozoa, and the lower fraction (10% BSA) was used for Y-spermatozoa. The observed variables included the percentage of progressive motility, viability, membrane integrity, acrosome integrity, DNA fragmentation, and spermatozoa kinematics. One-way ANOVA was used to analyse differences in semen quality among bulls. The results showed no significant differences (p<0.05) in spermatozoa quality or DNA fragmentation between sexed and non-sexed frozen semen. However, significant differences (p<0.05) were found in the kinematic parameters ALH and BCF among non-sexed, sexed-X, and sexed-Y semen. Overall, the BSA sexing method did not reveal notable differences in semen quality and DNA fragmentation between sexed and non-sexed spermatozoa.

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  • IPB University
  • National Research and Innovation Agency
  • University of Indonesia
  • Indonesian Institute of Sciences
  • Diponegoro University
  • University of Hawaiʻi at Mānoa
  • Universitas Halu Oleo
  • Afiliasi tidak diketahui
  • Organisasi berbeda
  • Organisasi sama
  • Afiliasi tidak diketahui
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Cece SumantriIPB University9
Rudy PriyantoIPB University9
Ronny Rachman NoorIPB University8
Mokhamad Fakhrul UlumIPB University4
Tulus MaulanaNational Research and Innovation Agency4
Raden Iis ArifiantiniIPB University4
Asep GunawanIPB University3
Kasita ListyariniIPB University3
Wasmen ManaluIPB University3
Ikhsan SuhendroIPB University3
Saiful AnwarNational Research and Innovation Agency3
Widya Pintaka Bayu PutraNational Research and Innovation Agency2
Paskah Partogi AgungIndonesian Institute of Sciences2
Ekayanti Mulyawati KaiinNational Research and Innovation Agency2
Fuadi ZulkipliIPB University1
Edwar EdwarAfiliasi tidak diketahui1
Sutikno SutiknoDiponegoro University1
Mutasem Ali M. AbuzahraAfiliasi tidak diketahui1
Muslimiah MuslimiahIPB University1
Irma Isnafia AriefIPB University1
Isyana KhaerunnisaNational Research and Innovation Agency1
Ahmad FurqonNational Research and Innovation Agency1
Cahyo BudimanIPB University1
Yong Soo KimUniversity of Hawaiʻi at Mānoa1
Dalilah RamadhaniIPB University1
Anik Budhi DharmayanthiNational Research and Innovation Agency1
S SetioriniUniversity of Indonesia1
Amaitshaa AdellaUniversity of Indonesia1
Indah SariUniversity of Indonesia1
Nursafira FathaniahUniversity of Indonesia1
Takdir SailiUniversitas Halu Oleo1
Dondin SajuthiIPB University1
Ariantie OSIPB University1
TL YusufAfiliasi tidak diketahui1
Khalis Talitha GusmanIPB University1
Muhammad - AgilIPB University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Comparative Analysis of Semen Quality and Kinematics in Sexed andNon-Sexed Sperm of Limousin Bulls</dc:title>
	<dc:creator xml:lang="en">Syarif, I. W.</dc:creator>
	<dc:creator xml:lang="en">Arifiantini, R. I.</dc:creator>
	<dc:creator xml:lang="en">Said, S.</dc:creator>
	<dc:creator xml:lang="en">Jakaria, J.</dc:creator>
	<dc:subject xml:lang="en">BSA sedimentation</dc:subject>
	<dc:subject xml:lang="en">Limousin bull</dc:subject>
	<dc:subject xml:lang="en">sperm sexing</dc:subject>
	<dc:subject xml:lang="en">sperm kinematic</dc:subject>
	<dc:subject xml:lang="en">semen quality</dc:subject>
	<dc:description xml:lang="en">The application of spermatozoa sexing technology offers benefits in determining the desired sex in cattle. However, this technology requires particular attention to quality, DNA fragmentation, and kinematics of spermatozoa. This study aimed to evaluate the effect of sexed and non-sexed semen on spermatozoa quality, DNA fragmentation, and kinematics in Limousin bulls. Four Limousin bulls between the ages of four and seven years and weighing between 610 and 814 kg were used in this study. The sexing method followed the bovine serum albumin (BSA) protocol. The upper fraction (5% BSA) was used for X-spermatozoa, and the lower fraction (10% BSA) was used for Y-spermatozoa. The observed variables included the percentage of progressive motility, viability, membrane integrity, acrosome integrity, DNA fragmentation, and spermatozoa kinematics. One-way ANOVA was used to analyse differences in semen quality among bulls. The results showed no significant differences (p&amp;lt;0.05) in spermatozoa quality or DNA fragmentation between sexed and non-sexed frozen semen. However, significant differences (p&amp;lt;0.05) were found in the kinematic parameters ALH and BCF among non-sexed, sexed-X, and sexed-Y semen. Overall, the BSA sexing method did not reveal notable differences in semen quality and DNA fragmentation between sexed and non-sexed spermatozoa.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2026-02-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/68593</dc:identifier>
	<dc:identifier>10.5398/tasj.2026.49.2.114</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 49 No. 2 (2026): Tropical Animal Science Journal; 114</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2026.49.2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/68593/32654</dc:relation>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/68593/33988</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Tropical Animal Science Journal</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>