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Double-Dose Artificial Insemination and Sperm Kinematics of Percoll Density Gradient-Sexed Semen in Holstein-Friesian Cattle

Tropical Animal Science Journal · Vol. 49 No. 5 (2026)

DOI
10.5398/tasj.2026.49.5.381
ISSN
2615-790X, 2615-787X
Terbit
2026-07-30
Penerbit
Faculty of Animal Science, IPB University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

This study aimed to evaluate the effect of sperm sexing using the Percoll density gradient centrifugation (PDGC) method on the kinematic, reproductive performance, and sex ratio of calves in Holstein-Friesian (HF) cattle. The study used three treatments: conventional semen (G0), PDGC sexed semen at 20%–60% (G1), and PDGC sexed semen at 20%–65% (G2). Kinematic variables were analyzed using a computer-assisted sperm analysis (CASA) system, while reproductive performance was evaluated based on non-return rate (NRR), conception rate (CR), calving rate (CvR), and sex ratio. Insemination in the sexed semen group was performed using a double-dose method to compensate for the potential decrease in viable sperm. The results showed that the PDGC method significantly affected several kinematic variables, particularly motility, distance average path (DAP), distance straight line (DSL), distance curved line (DCL), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), Wobble (WOB), amplitude of lateral head movement (ALH), and beat cross frequency (BCF) (p<0.05), reflecting changes in sperm movement dynamics due to the density-based separation process. However, there were no significant differences in reproductive performance variables between treatments, indicating that PDGC-based sexing does not significantly reduce in vivo fertilization capacity. The use of sexed semen increases the proportion of female offspring compared to conventional semen. Sexing sperm using the PDGC method is a relatively safe and applicable approach to increase the chances of female offspring without sacrificing reproductive efficiency, especially when combined with a double-dose insemination strategy.

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  • Hasanuddin University
  • State University of Makassar
  • Universitas Sulawesi Tenggara
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Rekan penulisOrganisasiArtikel bersama
MashudiAfiliasi tidak diketahui6
SuyadiAfiliasi tidak diketahui6
T. E. SusiloriniAfiliasi tidak diketahui6
M. Rifa’iAfiliasi tidak diketahui6
F. SafaAfiliasi tidak diketahui4
N. FebriantoAfiliasi tidak diketahui4
A. RachmawatiAfiliasi tidak diketahui4
A. YuswatiAfiliasi tidak diketahui4
A. AmaliyaAfiliasi tidak diketahui4
D. SulistyowatiAfiliasi tidak diketahui4
JunaediAfiliasi tidak diketahui3
M. H. NatsirAfiliasi tidak diketahui2
Rista Nikmatu RohmahAfiliasi tidak diketahui1
Soraya WidyasariAfiliasi tidak diketahui1
F. FatchiyahAfiliasi tidak diketahui1
Agus Hadi PrayitnoAfiliasi tidak diketahui1
Rusman RusmanHasanuddin University1
Sri HartatikAfiliasi tidak diketahui1
Edi SuryantoAfiliasi tidak diketahui1
Joni Murti Mulyo AjiAfiliasi tidak diketahui1
Y ErwantoAfiliasi tidak diketahui1
T LindriantiAfiliasi tidak diketahui1
KhaeruddinState University of Makassar1
M YusufUniversitas Sulawesi Tenggara1
G. CiptadiAfiliasi tidak diketahui1
A. H. FattahAfiliasi tidak diketahui1
B. SyamsuryadiAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Double-Dose Artificial Insemination and Sperm Kinematics of Percoll Density Gradient-Sexed Semen in Holstein-Friesian Cattle</dc:title>
	<dc:creator xml:lang="en">Utami, P.</dc:creator>
	<dc:creator xml:lang="en">Yekti, A. P. A.</dc:creator>
	<dc:creator xml:lang="en">Syah, H. A.</dc:creator>
	<dc:creator xml:lang="en">Pramudhita, A. D.</dc:creator>
	<dc:creator xml:lang="en">Ammaliya, A.</dc:creator>
	<dc:creator xml:lang="en">Aulanni’am, A.</dc:creator>
	<dc:creator xml:lang="en">Wahjuningsih, S.</dc:creator>
	<dc:creator xml:lang="en">Kusmartono, K.</dc:creator>
	<dc:creator xml:lang="en">Isnaini, N.</dc:creator>
	<dc:creator xml:lang="en">Siswoyo, T. A.</dc:creator>
	<dc:creator xml:lang="en">Susilawati, T.</dc:creator>
	<dc:subject xml:lang="en">artificial insemination</dc:subject>
	<dc:subject xml:lang="en">Holstein-Friesian</dc:subject>
	<dc:subject xml:lang="en">kinematic characteristic</dc:subject>
	<dc:subject xml:lang="en">sexed semen</dc:subject>
	<dc:subject xml:lang="en">sperm quality</dc:subject>
	<dc:description xml:lang="en">This study aimed to evaluate the effect of sperm sexing using the Percoll density gradient centrifugation (PDGC) method on the kinematic, reproductive performance, and sex ratio of calves in Holstein-Friesian (HF) cattle. The study used three treatments: conventional semen (G0), PDGC sexed semen at 20%–60% (G1), and PDGC sexed semen at 20%–65% (G2). Kinematic variables were analyzed using a computer-assisted sperm analysis (CASA) system, while reproductive performance was evaluated based on non-return rate (NRR), conception rate (CR), calving rate (CvR), and sex ratio. Insemination in the sexed semen group was performed using a double-dose method to compensate for the potential decrease in viable sperm. The results showed that the PDGC method significantly affected several kinematic variables, particularly motility, distance average path (DAP), distance straight line (DSL), distance curved line (DCL), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), Wobble (WOB), amplitude of lateral head movement (ALH), and beat cross frequency (BCF) (p&amp;lt;0.05), reflecting changes in sperm movement dynamics due to the density-based separation process. However, there were no significant differences in reproductive performance variables between treatments, indicating that PDGC-based sexing does not significantly reduce in vivo fertilization capacity. The use of sexed semen increases the proportion of female offspring compared to conventional semen. Sexing sperm using the PDGC method is a relatively safe and applicable approach to increase the chances of female offspring without sacrificing reproductive efficiency, especially when combined with a double-dose insemination strategy.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2026-07-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:format>text/xml</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/71965</dc:identifier>
	<dc:identifier>10.5398/tasj.2026.49.5.381</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 49 No. 5 (2026): Tropical Animal Science Journal; 381</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2026.49.5</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/71965/33918</dc:relation>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/71965/34010</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Tropical Animal Science Journal</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
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