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Hexane neem leaf extract more potent than ethanol extract against Aspergillus flavus

Universa Medicina · Vol. 33 No. 3 (2014) · hlm. 171–178

DOI
10.18051/UnivMed.2014.v33.171-178
ISSN
2407-2230, 1907-3062
Terbit
2014-12-02
Penerbit
Faculty of Medicine, Universitas Trisakti
Bahasa
eng

Abstrak

BACKGROUND Aspergillus flavus is one of the causes of aspergillosis, with a high virulence and resistance to standard antifungals, resulting in a high mortality rate. Medicinal plants are increasingly used as they are relatively safer with minimal side effects. Previously we found that the ethanol extract of neem (Azadirachta indica A Juss) leaves inhibits A. flavus growth in vitro. However, most chemical compounds with antifungal effect are nonpolar. The purpose of this research was to compare the antifungal effect of neem leaves extracted in a nonpolar solvent to that of leaves extracted in a polar solvent. METHODS An in vitro experimental research was conducted between October 2013 and January 2014. Neem leaves were extracted in ethanol or hexane at various concentrations. A macrodilution test with 48-hour incubation time was done in triplicate on 8 groups of samples. These comprised the neem leaf ethanol extract (NLEE) at 0.5, 1.0, and 2.0 g/dL, neem leaf hexane extract (NLHE) at 0.5, 1.0, and 2.0 g/dL, positive control, and negative control groups. Fungal growth was detected on Sabouroud dextrose agar. Statistical analysis used Chi square and Fisher’s exact test. RESULTS NLHE had a higher, but statistically non-significant, inhibitory effect on A. flavus than NLEE (p=0.996). At higher concentrations, the antifungal effect of NLHE is better than that of NLEE. CONCLUSION There is no significant difference in in-vitro inhibitory effectivity on A. flavus of neem leaves between extracts in polar and nonpolar solvents.

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  • Atma Jaya Catholic University of Indonesia
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Rekan penulisOrganisasiArtikel bersama
Rita DewiAtma Jaya Catholic University of Indonesia1
Ingrid M. MakahindaAtma Jaya Catholic University of Indonesia1
Maureen ApriliaAtma Jaya Catholic University of Indonesia1
Nikki SanjayaAtma Jaya Catholic University of Indonesia1
Harry GunawanAtma Jaya Catholic University of Indonesia1
Ayly MargaretAtma Jaya Catholic University of Indonesia1
Lies K. WibisonoAtma Jaya Catholic University of Indonesia1
Meiliyana WijayaAtma Jaya Catholic University of Indonesia1
Sem Samuel SurjaAtma Jaya Catholic University of Indonesia1
Melyawati HermawanAtma Jaya Catholic University of Indonesia1
Pramanta PramantaAtma Jaya Catholic University of Indonesia1

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Sumber metadata: OAI-PMH (metadata jurnal) https://univmed.org/ejurnal/index.php/medicina/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Hexane neem leaf extract more potent than ethanol extract against Aspergillus flavus</dc:title>
	<dc:creator xml:lang="en">Hidayat, Jenny</dc:creator>
	<dc:creator xml:lang="en">Margaret, Ay Ly</dc:creator>
	<dc:creator xml:lang="en">Yolanda, Hanna</dc:creator>
	<dc:creator xml:lang="en">Wibisono, Lies K.</dc:creator>
	<dc:subject xml:lang="en">Azadirachta indica</dc:subject>
	<dc:subject xml:lang="en">neem</dc:subject>
	<dc:subject xml:lang="en">A. flavus</dc:subject>
	<dc:subject xml:lang="en">ethanol</dc:subject>
	<dc:subject xml:lang="en">hexane</dc:subject>
	<dc:subject xml:lang="en">antifungal</dc:subject>
	<dc:description xml:lang="en">BACKGROUND Aspergillus flavus is one of the causes of aspergillosis, with a high virulence and resistance to standard antifungals, resulting in a high mortality rate. Medicinal plants are increasingly used as they are relatively safer with minimal side effects. Previously we found that the ethanol extract of neem (Azadirachta indica A Juss) leaves inhibits A. flavus growth in vitro. However, most chemical compounds with antifungal effect are nonpolar. The purpose of this research was to compare the antifungal effect of neem leaves extracted in a nonpolar solvent to that of leaves extracted in a polar solvent. METHODS An in vitro experimental research was conducted between October 2013 and January 2014. Neem leaves were extracted in ethanol or hexane at various concentrations. A macrodilution test with 48-hour incubation time was done in triplicate on 8 groups of samples. These comprised the neem leaf ethanol extract (NLEE) at 0.5, 1.0, and 2.0 g/dL, neem leaf hexane extract (NLHE) at 0.5, 1.0, and 2.0 g/dL, positive control, and negative control groups. Fungal growth was detected on Sabouroud dextrose agar. Statistical analysis used Chi square and Fisher’s exact test. RESULTS NLHE had a higher, but statistically non-significant, inhibitory effect on A. flavus than NLEE (p=0.996). At higher concentrations, the antifungal effect of NLHE is better than that of NLEE. CONCLUSION There is no significant difference in in-vitro inhibitory effectivity on A. flavus of neem leaves between extracts in polar and nonpolar solvents.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Medicine, Universitas Trisakti</dc:publisher>
	<dc:date>2014-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://univmed.org/ejurnal/index.php/medicina/article/view/13</dc:identifier>
	<dc:identifier>10.18051/UnivMed.2014.v33.171-178</dc:identifier>
	<dc:source xml:lang="en">Universa Medicina; Vol. 33 No. 3 (2014); 171-178</dc:source>
	<dc:source>2407-2230</dc:source>
	<dc:source>1907-3062</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://univmed.org/ejurnal/index.php/medicina/article/view/13/10</dc:relation>
	<dc:relation>10.18051/univmed.v33i3.13</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2014 Jenny Hidayat, Ay Ly Margaret, Hanna Yolanda, Lies K. Wibisono</dc:rights>
</oai_dc:dc>