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Antifungal activity of neem leaf ethanol extract on Aspergillus flavus

Universa Medicina · Vol. 32 No. 2 (2013) · hlm. 80–85

DOI
10.18051/UnivMed.2013.v32.80-85
ISSN
2407-2230, 1907-3062
Terbit
2013-08-14
Penerbit
Faculty of Medicine, Universitas Trisakti
Bahasa
eng

Abstrak

BACKGROUNDAspergillosis is an infectious disease due to inhalation of Aspergillus spores.Drug resistance to aspergillosis has been reported since 14 years ago. Therefore,alternative treatment using herbals began to attract the attention of the medicalprofession and the community, because herbals are relatively safe and haveminimal side effects. Neem (Azadirachta indica) is an herb with broad spectrumbiological activity, but studies on the antifungal activity of neem leaves arelacking. The objective of this study was to evaluate the activity of neem leaveson Aspergillus flavus (A. flavus).METHODSThis was a laboratory experimental study using A. flavus samples obtained frombronchial washing specimens of inpatients. Neem leaf extract in ethanol (NLE)was prepared in various concentrations. Aspergillus. flavus antifungal activityof NLE were assessed by disc diffusion (using NLE at 1.0; 0.75; 0.50; 0.25 g/dL)and macrodilution (using NLE at 1.0; 0.75; 0.5; 0.25; 0.125 g/dL). Ketoconazole0.1 g/dL was used as antifungal positive control. Disc diffusion and macrodilutionwere conducted using Sabouraud dextrose agar and broth, to measure inhibitionzone diameter and minimal inhibitory concentration (MIC), respectively. Statisticalanalysis was performed using Mann-Whitney test from SPSS 15.RESULTSThere were significant differences in inhibition zone diameter (p = 0.034) betweenA. flavus samples exposed to NLE (1 g/dL) and the control group exposed toketoconazole (0.1 g/dL). The MIC of NLE against A. flavus was 0.5 g/dL.CONCLUSIONSNeem leaf ethanol extract has antifungal activity against A.flavus in vitro.

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  • Atma Jaya Catholic University of Indonesia
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Rekan penulisOrganisasiArtikel bersama
Rita DewiAtma Jaya Catholic University of Indonesia1
Ingrid M. MakahindaAtma Jaya Catholic University of Indonesia1
Maureen ApriliaAtma Jaya Catholic University of Indonesia1
Nikki SanjayaAtma Jaya Catholic University of Indonesia1
Harry GunawanAtma Jaya Catholic University of Indonesia1
Lies K. WibisonoAfiliasi tidak diketahui1
Jenny HidayatAtma Jaya Catholic University of Indonesia1
Ay Ly MargaretAtma Jaya Catholic University of Indonesia1
Meiliyana WijayaAtma Jaya Catholic University of Indonesia1
Sem Samuel SurjaAtma Jaya Catholic University of Indonesia1
Melyawati HermawanAtma Jaya Catholic University of Indonesia1
Pramanta PramantaAtma Jaya Catholic University of Indonesia1

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Sumber metadata: OAI-PMH (metadata jurnal) https://univmed.org/ejurnal/index.php/medicina/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Antifungal activity of neem leaf ethanol extract on Aspergillus flavus</dc:title>
	<dc:creator xml:lang="en">Margaret, Ayly</dc:creator>
	<dc:creator xml:lang="en">Yolanda, Hanna</dc:creator>
	<dc:creator xml:lang="en">Lies K, Wibisono</dc:creator>
	<dc:subject xml:lang="en">A. flavus</dc:subject>
	<dc:subject xml:lang="en">neem</dc:subject>
	<dc:subject xml:lang="en">Azadirachta indica</dc:subject>
	<dc:subject xml:lang="en">antifungal</dc:subject>
	<dc:description xml:lang="en">BACKGROUNDAspergillosis is an infectious disease due to inhalation of Aspergillus spores.Drug resistance to aspergillosis has been reported since 14 years ago. Therefore,alternative treatment using herbals began to attract the attention of the medicalprofession and the community, because herbals are relatively safe and haveminimal side effects. Neem (Azadirachta indica) is an herb with broad spectrumbiological activity, but studies on the antifungal activity of neem leaves arelacking. The objective of this study was to evaluate the activity of neem leaveson Aspergillus flavus (A. flavus).METHODSThis was a laboratory experimental study using A. flavus samples obtained frombronchial washing specimens of inpatients. Neem leaf extract in ethanol (NLE)was prepared in various concentrations. Aspergillus. flavus antifungal activityof NLE were assessed by disc diffusion (using NLE at 1.0; 0.75; 0.50; 0.25 g/dL)and macrodilution (using NLE at 1.0; 0.75; 0.5; 0.25; 0.125 g/dL). Ketoconazole0.1 g/dL was used as antifungal positive control. Disc diffusion and macrodilutionwere conducted using Sabouraud dextrose agar and broth, to measure inhibitionzone diameter and minimal inhibitory concentration (MIC), respectively. Statisticalanalysis was performed using Mann-Whitney test from SPSS 15.RESULTSThere were significant differences in inhibition zone diameter (p = 0.034) betweenA. flavus samples exposed to NLE (1 g/dL) and the control group exposed toketoconazole (0.1 g/dL). The MIC of NLE against A. flavus was 0.5 g/dL.CONCLUSIONSNeem leaf ethanol extract has antifungal activity against A.flavus in vitro.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Medicine, Universitas Trisakti</dc:publisher>
	<dc:date>2013-08-14</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://univmed.org/ejurnal/index.php/medicina/article/view/62</dc:identifier>
	<dc:identifier>10.18051/UnivMed.2013.v32.80-85</dc:identifier>
	<dc:source xml:lang="en">Universa Medicina; Vol. 32 No. 2 (2013); 80-85</dc:source>
	<dc:source>2407-2230</dc:source>
	<dc:source>1907-3062</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://univmed.org/ejurnal/index.php/medicina/article/view/62/77</dc:relation>
	<dc:relation>10.18051/univmed.v32i2.62</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2015 Ayly Margaret, Hanna Yolanda, Wibisono Lies K</dc:rights>
</oai_dc:dc>