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Curcumin inhibits luteal cell steroidogenesis by suppression of extracellular signal regulated kinase

Universa Medicina · Vol. 31 No. 2 (2012) · hlm. 73–80

DOI
10.18051/UnivMed.2012.v31.73-80
ISSN
2407-2230, 1907-3062
Terbit
2012-08-14
Penerbit
Faculty of Medicine, Universitas Trisakti
Bahasa
eng

Abstrak

BackgroundCurcumin inhibits steroidogenesis in luteal cell cultures by inhibiting progesterone secretion. The site of action of curcumin on steroidogenesis in luteal cell cultures is as yet unkown. The purpose of this study was to determine the influence of curcumin on phosphorylation of extracellular signal regulated kinase (ERK) in steroidogenesis of luteal cell cultures. Methods An experimental study with control was conducted to investigate the site of action of curcumin by measurement of ERK phosphorylation in luteal cell cultures (LCC) after administration of luteinizing hormone (LH) and/or prostaglandin F2 alpha (PGF2á). The subjects of this study were luteal cell cultures derived from the corpus luteum of Sprague Dawley rat with superovulation induced by pregnant mare serum gonadotropin. The luteal cell cultures were devided into 16 groups, both with and without the addition of forskolin and each group contained 4 replicates samples. Curcumin was administered immediately following LH and/or PGF2á stimulation with or without addition of forskolin. The cell culture was then incubated for 24 h. ERK phosphorylation was measured by immunohistochemistry. Data on ERK phosphorylation was analyzed using one-way Anova, followed by multiple comparison tests. ResultsLH significantly increased ERK phosphorylation, whereas PGF2á significantly reduced ERK phosphorylation. Forskolin significantly increased ERK phosphorylation to a similar degree as LH. Curcumin inhibited ERK phosphorylation in both LH and forskolin-stimulated luteal cell cultures. ConclusionCurcumin inhibits ERK phosphorylation in luteal cell cultures by suppressing signal transduction upstream of ERK.

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  • Universitas Gadjah Mada
  • Yarsi University
  • Indonesian Institute of Sciences
  • Kementerian Kesehatan Republik Indonesia
  • Universitas Jember
  • Nara Institute of Science and Technology
  • Atma Jaya University Yogyakarta
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Rekan penulisOrganisasiArtikel bersama
Ratna Asmah SusidartiUniversitas Gadjah Mada17
Adam HermawanUniversitas Gadjah Mada17
Riris Istighfari JenieUniversitas Gadjah Mada15
Muthi IkawatiUniversitas Gadjah Mada11
Rohmad Yudi UtomoUniversitas Gadjah Mada8
Dyaningtyas Dewi Pamungkas PutriUniversitas Gadjah Mada7
Faaza Aulia RahmanUniversitas Gadjah Mada5
Sari HaryantiKementerian Kesehatan Republik Indonesia5
Dhania NovitasariUniversitas Gadjah Mada4
Retno MurwantiUniversitas Gadjah Mada4
Desty Restia RahmawatiUniversitas Gadjah Mada4
Sri HandayaniIndonesian Institute of Sciences3
Ummi Maryam ZulfinUniversitas Gadjah Mada3
Anindyajati AnindyajatiUniversitas Gadjah Mada3
Larasati LarasatiUniversitas Gadjah Mada3
Erlina RivantiUniversitas Gadjah Mada3
Etty WidayantiYarsi University3
Yulia Suciati KoharYarsi University3
Masashi KawaichiNara Institute of Science and Technology2
Sendy JunediAtma Jaya University Yogyakarta2
Nunuk Aries NurulitaMuhammadiyah University Purwokerto2
Suwijiyo PramonoUniversitas Gadjah Mada2
Zalinar UdinIndonesian Institute of Sciences2
Yogi ErtantoUniversitas Gadjah Mada2
Lailatul QodriaUniversitas Gadjah Mada2
Gergorius Gena MaranUniversitas Gadjah Mada2
Aulia Nur SeptianiUniversitas Gadjah Mada2
Sofa FaridaUniversitas Gadjah Mada2
Beni LestariUniversitas Gadjah Mada2
Asri Mega PutriUniversitas Gadjah Mada2
Hilyatul FadliyahUniversitas Gadjah Mada2
Indri KusharyantiTanjungpura University2
Yonika Arum LarasatiUniversitas Gadjah Mada2
Raditya Prima IstiajiUniversitas Gadjah Mada2
Ni Putu Linda LaksmianiUdayana University2
Banun KusumawardaniUniversitas Jember2

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Sumber metadata: OAI-PMH (metadata jurnal) https://univmed.org/ejurnal/index.php/medicina/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Curcumin inhibits luteal cell steroidogenesis by  suppression of extracellular signal regulated kinase</dc:title>
	<dc:creator xml:lang="en">Purwaningsih, Endang</dc:creator>
	<dc:creator xml:lang="en">Soejono, Sri Kadarsih</dc:creator>
	<dc:creator xml:lang="en">Dasuki, Djaswadi</dc:creator>
	<dc:creator xml:lang="en">Meiyanto, Edy</dc:creator>
	<dc:subject xml:lang="en">Curcumin</dc:subject>
	<dc:subject xml:lang="en">signal transduction</dc:subject>
	<dc:subject xml:lang="en">forskolin</dc:subject>
	<dc:subject xml:lang="en">luteal cells culture</dc:subject>
	<dc:description xml:lang="en">BackgroundCurcumin inhibits steroidogenesis in luteal cell cultures by inhibiting progesterone secretion. The site of action of curcumin on steroidogenesis in luteal cell cultures is as yet unkown. The purpose of this study was to determine the influence of curcumin on phosphorylation of extracellular signal regulated kinase (ERK) in steroidogenesis of luteal cell cultures.     Methods An experimental study with control was conducted to investigate the site of action of curcumin by measurement of ERK phosphorylation in luteal cell cultures (LCC) after administration of luteinizing hormone (LH) and/or prostaglandin F2 alpha (PGF2á). The subjects of this study were luteal cell cultures derived from the corpus luteum of Sprague Dawley rat with superovulation induced by pregnant mare serum gonadotropin. The luteal cell cultures were devided into 16 groups, both with and without the addition of forskolin and each group contained 4 replicates samples. Curcumin was administered immediately following LH and/or PGF2á stimulation with or without addition of forskolin. The cell culture was then incubated for 24 h. ERK phosphorylation was measured by immunohistochemistry. Data on ERK phosphorylation was analyzed using one-way Anova, followed by multiple comparison tests.     ResultsLH significantly increased ERK phosphorylation, whereas PGF2á significantly reduced ERK phosphorylation. Forskolin significantly increased ERK phosphorylation to a similar degree as LH. Curcumin inhibited ERK phosphorylation in both LH and forskolin-stimulated luteal cell cultures. ConclusionCurcumin inhibits ERK phosphorylation in luteal cell cultures by suppressing signal transduction upstream of ERK.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Medicine, Universitas Trisakti</dc:publisher>
	<dc:date>2012-08-14</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://univmed.org/ejurnal/index.php/medicina/article/view/98</dc:identifier>
	<dc:identifier>10.18051/UnivMed.2012.v31.73-80</dc:identifier>
	<dc:source xml:lang="en">Universa Medicina; Vol. 31 No. 2 (2012); 73-80</dc:source>
	<dc:source>2407-2230</dc:source>
	<dc:source>1907-3062</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://univmed.org/ejurnal/index.php/medicina/article/view/98/109</dc:relation>
	<dc:relation>10.18051/univmed.v31i2.98</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2015 Endang Purwaningsih, Sri Kadarsih Soejono, Djaswadi Dasuki, Edy Meiyanto</dc:rights>
</oai_dc:dc>