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Hypoxia enhances self-renewal properties and markers of mesenchymal stem cells

Universa Medicina · Vol. 38 No. 3 (2019) · hlm. 164–171

DOI
10.18051/UnivMed.2019.v38.164-171
ISSN
2407-2230, 1907-3062
Terbit
2019-10-16
Penerbit
Faculty of Medicine, Universitas Trisakti
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc-sa/4.0

Abstrak

BackgroundMesenchymal stem cells (MSCs) are multipotent stromal cells that express CD73, CD90, and CD105 surface markers, but not CD14, CD45, CD34, CD11b, and HLA-DR. MSCs under hypoxic conditions have the essential role of maintaining the stemness capacity by releasing several growth factors into their medium, known as hypoxia conditioned medium (HCM). This study was performed to compare the effect of percentage of HCM to normoxic medium (NM) in increasing MSC proliferation marked by proliferation rate and surface marker expression.MethodsThis study was of post-test only control group design using human umbilical cord-MSCs (hUC-MSCs) as subjects. The HCM treatment group was obtained by culturing MSCs under 5% O2, whereas the NM control group was grown under 20% O2. The hUC-MSCs were divided into 4 groups with different dose ratios of HCM to NM (25%:75%; 50%:50%; 75%:25% for P1, P2 and P3, respectively and 100% of NM for the controls). All of these groups were maintained at 37oC and the data was collected after 72 hours incubation. MSC marker expression of CD73, CD90 and CD105 was analyzed using flow cytometry and MSC proliferation by trypan blue assay. ResultThere were significant differences in MSC marker expression of CD73, CD90 and CD105 and proliferation at all dose ratios of HCM to NM (p<0.05).ConclusionLow oxygen concentration promotes MSC proliferation and stemness thus it might be beneficial for maintaining the MSC physiologic niche in-vitro.

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  • Sultan Agung Islamic University
  • Universitas Sumatera Utara
  • State University of Semarang
  • Soegijapranata Catholic University
  • University of Mataram
  • Diponegoro University
  • Universitas Prima Indonesia
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Rekan penulisOrganisasiArtikel bersama
Nurul HidayahSultan Agung Islamic University4
Nur Dina AmalinaState University of Semarang3
Setyo TrisnadiSultan Agung Islamic University3
Chodijah ChodijahSultan Agung Islamic University3
Nurul HidayahSultan Agung Islamic University3
Iffan AlifSultan Agung Islamic University3
Salma Yasmine AzzaharaSultan Agung Islamic University3
Adi Muradi MuharUniversitas Sumatera Utara2
Bayu Tirta DirjaUniversity of Mataram2
Risky Chandra Satria IrawanSultan Agung Islamic University2
Sugeng IbrahimSoegijapranata Catholic University2
Riska RachmaniaSultan Agung Islamic University2
Sofian Azalia HusainAfiliasi tidak diketahui2
Ade Indra MuktiUniversitas Prima Indonesia2
Reynaldi SuryajayaAfiliasi tidak diketahui2
Siti Thomas ZulaikhahSultan Agung Islamic University2
Joko Wahyu WibowoSultan Agung Islamic University2
Rachellicya Ristioni SihalohoSultan Agung Islamic University2
Shabrina Syifa GhaissaniSultan Agung Islamic University2
Alisia Martha DewiSultan Agung Islamic University1
Setyo TrisnasiSultan Agung Islamic University1
Hadi SarosaSultan Agung Islamic University1
Sri Priyantini MulyaniSultan Agung Islamic University1
Faizal MukharimSultan Agung Islamic University1
Dedy HermansyahUniversitas Sumatera Utara1
Yan Wisnu PrajokoDiponegoro University1
Pangesti DrawinaSultan Agung Islamic University1
Taufiqurrachman NasihunSultan Agung Islamic University1
Nur Anna Chalimah Sa'dyahAfiliasi tidak diketahui1
Chodidjah ChodidjahSultan Agung Islamic University1
Hadi SunartoSultan Agung Islamic University1
Arya TjiptaUniversitas Sumatera Utara1
Sahara MaharaniSultan Agung Islamic University1
Dedi HermansyahSultan Agung Islamic University1
Aditya NugrahaSultan Agung Islamic University1
Azizah Retno KustiyahSultan Agung Islamic University1
Nur Anna C Sa’dyahSultan Agung Islamic University1

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Sumber metadata: OAI-PMH (metadata jurnal) https://univmed.org/ejurnal/index.php/medicina/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Hypoxia enhances self-renewal properties  and markers of mesenchymal stem cells</dc:title>
	<dc:creator xml:lang="en">Yustianingsih, Vivi</dc:creator>
	<dc:creator xml:lang="en">Sumarawati, Titiek</dc:creator>
	<dc:creator xml:lang="en">Putra, Agung</dc:creator>
	<dc:subject xml:lang="en">MSC</dc:subject>
	<dc:subject xml:lang="en">MSC-HCM</dc:subject>
	<dc:subject xml:lang="en">proliferation</dc:subject>
	<dc:subject xml:lang="en">stemness</dc:subject>
	<dc:description xml:lang="en">BackgroundMesenchymal stem cells (MSCs) are multipotent stromal cells that express CD73, CD90, and CD105 surface markers, but not CD14, CD45, CD34, CD11b, and HLA-DR. MSCs under hypoxic conditions have the essential role of maintaining the stemness capacity by releasing several growth factors into their medium, known as hypoxia conditioned medium (HCM). This study was performed to compare the effect of percentage of HCM to normoxic medium (NM) in increasing MSC proliferation marked by proliferation rate and surface marker expression.MethodsThis study was of post-test only control group design using human umbilical cord-MSCs (hUC-MSCs) as subjects. The HCM treatment group was obtained by culturing MSCs under 5% O2, whereas the NM control group was grown under 20% O2. The hUC-MSCs were divided into 4 groups with different dose ratios of HCM to NM (25%:75%; 50%:50%; 75%:25% for P1, P2 and P3, respectively and 100% of NM for the controls). All of these groups were maintained at 37oC and the data was collected after 72 hours incubation. MSC marker expression of CD73, CD90 and CD105 was analyzed using flow cytometry and MSC proliferation by trypan blue assay. ResultThere were significant differences in MSC marker expression of CD73, CD90 and CD105 and proliferation at all dose ratios of HCM to NM (p&amp;lt;0.05).ConclusionLow oxygen concentration promotes MSC proliferation and stemness thus it might be beneficial for maintaining the MSC physiologic niche in-vitro.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Medicine, Universitas Trisakti</dc:publisher>
	<dc:date>2019-10-16</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://univmed.org/ejurnal/index.php/medicina/article/view/715</dc:identifier>
	<dc:identifier>10.18051/UnivMed.2019.v38.164-171</dc:identifier>
	<dc:source xml:lang="en">Universa Medicina; Vol. 38 No. 3 (2019); 164-171</dc:source>
	<dc:source>2407-2230</dc:source>
	<dc:source>1907-3062</dc:source>
	<dc:source>10.18051/UnivMed.2019.v38.i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://univmed.org/ejurnal/index.php/medicina/article/view/715/565</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 Vivi Yustianingsih, Titiek Sumarawati, Agung Putra</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
</oai_dc:dc>