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Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras

VALENSI · Vol. ume No. 1 (2015) · hlm. 26–32

DOI
10.15408/jkv.v0i0.3150
ISSN
2548-3013, 2460-6065
Terbit
2015-05-31
Penerbit
Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
Bahasa
eng

Abstrak

Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60°C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively. DOI:http://dx.doi.org/10.15408/jkv.v0i0.3150

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  • Syarif Hidayatullah State Islamic University Jakarta
  • National Nuclear Energy Agency of Indonesia
  • Bandung Institute of Technology
  • Universitas Mathla'ul Anwar Banten
  • Sunan Kalijaga State Islamic University Yogyakarta
  • University of Indonesia
  • Indonesia Defense University
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Rekan penulisOrganisasiArtikel bersama
Dede SukandarSyarif Hidayatullah State Islamic University Jakarta5
Tarso RudianaUniversitas Mathla'ul Anwar Banten3
Irawan SugoroNational Nuclear Energy Agency of Indonesia3
Anna MuawanahSyarif Hidayatullah State Islamic University Jakarta2
Asih Widi WisudawatiSunan Kalijaga State Islamic University Yogyakarta1
Muhammad Ihda H.L. ZeinSyarif Hidayatullah State Islamic University Jakarta1
Ahmad FathoniSyarif Hidayatullah State Islamic University Jakarta1
Barita Juliano SiregarSyarif Hidayatullah State Islamic University Jakarta1
Denny AlfarusSyarif Hidayatullah State Islamic University Jakarta1
Abdul MutalibPadjadjaran University1
Yahdiana HarahapIndonesia Defense University1
Sunarhadijoso SoenarjoNational Nuclear Energy Agency of Indonesia1
Ratna Dini HaryuniNational Nuclear Energy Agency of Indonesia1
Anton BahtiarUniversity of Indonesia1
Martalena RamliNational Nuclear Energy Agency of Indonesia1
C.N. ArdiyatnoNational Nuclear Energy Agency of Indonesia1
Veronika Yulianti SusiloNational Nuclear Energy Agency of Indonesia1
D. HaffidNational Nuclear Energy Agency of Indonesia1
Ikmalia IkmaliaSyarif Hidayatullah State Islamic University Jakarta1
Emi LestariSyarif Hidayatullah State Islamic University Jakarta1
Rizkina HarahapSyarif Hidayatullah State Islamic University Jakarta1
Mirzan Thabrani RazzakSyarif Hidayatullah State Islamic University Jakarta1
Priyambodo PriyambodoSyarif Hidayatullah State Islamic University Jakarta1
Septyani NurichawatiSyarif Hidayatullah State Islamic University Jakarta1
Pingkan AditiawatiBandung Institute of Technology1
Dwiwahju SasongkoBandung Institute of Technology1
Dea Rizky IndrianiBandung Institute of Technology1
ROBIATUL ADAWIYAHUniversitas Nurul Jadid1
Prita WardhaniSyarif Hidayatullah State Islamic University Jakarta1
Imamah Al MabrurSyarif Hidayatullah State Islamic University Jakarta1
Cut Dhien K MeutiaSyarif Hidayatullah State Islamic University Jakarta1
Hilman AffandiSoutheast Asian Regional Centre for Tropical Biology1
Thamzil LasSyarif Hidayatullah State Islamic University Jakarta1
Farhat EtriyaSyarif Hidayatullah State Islamic University Jakarta1
Eddy JusufIndonesian Institute of Sciences1
M. Hero ShiddiqiSyarif Hidayatullah State Islamic University Jakarta1
Tiah MaharaniSyarif Hidayatullah State Islamic University Jakarta1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.uinjkt.ac.id/index.php/valensi/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras</dc:title>
	<dc:creator xml:lang="en">Hermanto, Sandra</dc:creator>
	<dc:creator xml:lang="en">Saputra, Fahrur Rahman</dc:creator>
	<dc:creator xml:lang="en">., Zilhadia</dc:creator>
	<dc:subject xml:lang="en">gelatin</dc:subject>
	<dc:subject xml:lang="en">hydrolysis</dc:subject>
	<dc:subject xml:lang="en">hard capsules</dc:subject>
	<dc:subject xml:lang="en">pepsin</dc:subject>
	<dc:subject xml:lang="en">SDS PAGE</dc:subject>
	<dc:description xml:lang="en">Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60°C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively. DOI :http://dx.doi.org/10.15408/jkv.v0i0.3150</dc:description>
	<dc:publisher xml:lang="en">Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University</dc:publisher>
	<dc:date>2015-05-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinjkt.ac.id/index.php/valensi/article/view/3150</dc:identifier>
	<dc:identifier>10.15408/jkv.v0i0.3150</dc:identifier>
	<dc:source xml:lang="en">Jurnal Kimia Valensi; Jurnal Kimia VALENSI Volume 1, No. 1, Mei 2015; 26-32</dc:source>
	<dc:source>2548-3013</dc:source>
	<dc:source>2460-6065</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinjkt.ac.id/index.php/valensi/article/view/3150/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 Sandra Hermanto, Fahrur Rahman Saputra, Zilhadia .</dc:rights>
</oai_dc:dc>