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Pengaruh Jumlah Inokulum Sel Inang Bakteri E.coli BL21 (DE3) pLysS dan Waktu Overekspresi pada Produksi Protein Rekombinan Fim-C Salmonella typhi

VALENSI · Vol. ume No. 2 (2018) · hlm. 98–106

DOI
10.15408/jkv.v4i2.8077
ISSN
2548-3013, 2460-6065
Terbit
2018-11-30
Penerbit
Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
Bahasa
eng

Abstrak

Recombinant protein Fim-C S.typhi is a potential protein that can be used as an alternative typhoid vaccine and produced on a large scale. However, before entering into a large-scale stage, laboratory optimum data on the factors that affect the production process of Fim-C Salmonella typhi proteins are required. This study aims to obtain information regarding the effect of host cell number E.coli BL21 (DE3) pLysS and overexpression time on production of recombinant protein Fim-C Salmonella typhi as the basis for developing candidate of typhoid vaccine. The optimization process of overexpression was done by adding 0.5 mM IPTG (Isopropyl-β-D-thiogalactopyranoside) inducer into bacterial cultures of 2%, 4%, and 6% with 4, 5, and 6 hours over expression. The measurement of the concentration of Fim-C recombinant protein extracted samples were done by a spectrophotometric method used BCA Kit Assay Thermo ScientificTM with wavelength 590nm. The characterization of those protein extracts was performed using SDS-PAGE method. The results from the study concluded that the number of 4% E.coli bacterial cells and four-hour overexpression time are the optimum condition of Fim- C Salmonella typhi characterized by the presence of high-intensity bands at ± 31 kDa.

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  • State University of Jakarta
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  • Universitas Airlangga
  • Arnold Palmer Hospital for Children
  • City of Scientific Research and Technological Applications
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Rekan penulisOrganisasiArtikel bersama
Fera KurniadewiState University of Jakarta8
Irma Ratna KartikaState University of Jakarta6
Gladys Indira PutriState University of Jakarta5
Jefferson Lynford DeclanState University of Jakarta5
Vira SaamiaUniversitas Airlangga5
Bassam AbomoelakArnold Palmer Hospital for Children4
Hesham Ali El EnshasyCity of Scientific Research and Technological Applications4
Dandy Akbar JuliansyahState University of Jakarta3
Adinda Myra Amalia PutriState University of Jakarta3
Atikah Nur RahmawatiState University of Jakarta3
I Made WiranathaAfiliasi tidak diketahui3
NovitasariAfiliasi tidak diketahui2
Anisa FitriyantiState University of Jakarta2
Rara SeruniState University of Jakarta2
Juwartina Ida RoyaniIPB University2
Tiara FahrizaState University of Jakarta2
Ayu BerkahingrumState University of Jakarta2
Dalia SukmawatiState University of Jakarta2
Maharanianska AzzahraState University of Jakarta2
Irvan MaulanaState University of Jakarta2
Gusti Angieta PutriState University of Jakarta2
Churiyah ChuriyahBadan Pengkajian dan Penerapan Teknologi2
Futi Kusuma HatiState University of Jakarta1
Nur Fadillah Adelia AzahraState University of Jakarta1
Fadhila Shofi AlbanaState University of Jakarta1
Afifah AisyafitriState University of Jakarta1
Dema Griseldis AurelliaState University of Jakarta1
Siti MunawaohState University of Jakarta1
Siti MunawarohState University of Jakarta1
Tri SusantiState University of Jakarta1
Efrida MartiusNational Research and Innovation Agency1
Tina RostinawatiPadjadjaran University1
Anna SafarridaNational Research and Innovation Agency1
Fahroziah AssyifaNational Research and Innovation Agency1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.uinjkt.ac.id/index.php/valensi/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Pengaruh Jumlah Inokulum Sel Inang Bakteri E.coli BL21 (DE3) pLysS dan Waktu Overekspresi pada Produksi Protein Rekombinan Fim-C Salmonella typhi</dc:title>
	<dc:creator xml:lang="en">Nurjayadi, Muktiningsih</dc:creator>
	<dc:creator xml:lang="en">Chairinnisa, Izzatul Ilma</dc:creator>
	<dc:creator xml:lang="en">Mentari, Geta Putri</dc:creator>
	<dc:creator xml:lang="en">Hardianto, Dudi</dc:creator>
	<dc:creator xml:lang="en">Sulfianti, Asri</dc:creator>
	<dc:creator xml:lang="en">Agustini, Kurnia</dc:creator>
	<dc:subject xml:lang="en">Recombinant protein Fim-C Salmonella typhi</dc:subject>
	<dc:subject xml:lang="en">Salmonella typhi</dc:subject>
	<dc:subject xml:lang="en">Overexpression</dc:subject>
	<dc:description xml:lang="en">Recombinant protein Fim-C S.typhi is a potential protein that can be used as an alternative typhoid vaccine and produced on a large scale. However, before entering into a large-scale stage, laboratory optimum data on the factors that affect the production process of Fim-C Salmonella typhi proteins are required. This study aims to obtain information regarding the effect of host cell number E.coli BL21 (DE3) pLysS and overexpression time on production of recombinant protein Fim-C Salmonella typhi as the basis for developing candidate of typhoid vaccine. The optimization process of overexpression was done by adding 0.5 mM IPTG (Isopropyl-β-D-thiogalactopyranoside) inducer into bacterial cultures of 2%, 4%, and 6% with 4, 5, and 6 hours over expression. The measurement of the concentration of Fim-C recombinant protein extracted samples were done by a spectrophotometric method used BCA Kit Assay Thermo ScientificTM with wavelength 590nm. The characterization of those protein extracts was performed using SDS-PAGE method. The results from the study concluded that the number of 4% E.coli bacterial cells and four-hour overexpression time are the optimum condition of Fim- C Salmonella typhi characterized by the presence of high-intensity bands at ± 31 kDa.  </dc:description>
	<dc:publisher xml:lang="en">Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University</dc:publisher>
	<dc:date>2018-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinjkt.ac.id/index.php/valensi/article/view/8077</dc:identifier>
	<dc:identifier>10.15408/jkv.v4i2.8077</dc:identifier>
	<dc:source xml:lang="en">Jurnal Kimia Valensi; Jurnal Kimia VALENSI Volume 4, No. 2, November 2018; 98-106</dc:source>
	<dc:source>2548-3013</dc:source>
	<dc:source>2460-6065</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinjkt.ac.id/index.php/valensi/article/view/8077/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 Muktiningsih Nurjayadi, Izzatul Ilma Chairinnisa, Geta Putri Mentari, Dudi Hardianto, Asri Sulfianti, Kurnia Agustini</dc:rights>
</oai_dc:dc>