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Isolation and Identification of Lactic Acid Bacteria form Chinese Cabbage Waste by 16s rRNA Amplification using The Polymerase Chain Reaction (PCR) Method

VALENSI · Vol. ume No. 1 (2023) · hlm. 136–144

DOI
10.15408/jkv.v9i1.29466
ISSN
2548-3013, 2460-6065
Terbit
2023-06-04
Penerbit
Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
Bahasa
eng

Abstrak

Waste is material that is disposed of from sources of human and natural activities that do not yet have economic value. Physically, vegetable waste is perishable due to its high-water content, especially Chinese Cabbage waste. The purpose of this study was the isolation and identification of lactic acid bacteria from Chinese Cabbage waste. Isolation was carried out to obtain isolates of lactic acid bacteria from Chinese Cabbage waste. Then it was amplified using a Polymerase Chain Reaction (PCR) instrument. The results of chromosomal DNA amplification of bacterial isolates from mustard 1, mustard 2 and mustard 3 waste showed DNA bands with a size of ± 1200 bp, so it can be concluded that the process of amplification of the 16s rRNA gene fragment in mustard 1, mustard 2 and mustard 3 isolates was successful. The results of the 16s rRNA base sequence analysis showed that the mustard 1 isolate had a similarity index of 84.65% with Bacillus sp, the gene for the mustard 2 isolate had a similarity index of 84.09% with the Uncultured bacterium clone, and the mustard 3 isolate gene had a similarity index of 85.42% with Environmental 16s rDNA sequence.

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  • Sunan Gunung Djati State Islamic University Bandung
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Rekan penulisOrganisasiArtikel bersama
Asep SupriadinSunan Gunung Djati State Islamic University Bandung3
Baiq Vera El VieraSunan Gunung Djati State Islamic University Bandung2
Vina AmaliaSunan Gunung Djati State Islamic University Bandung2
Soni SetiadjiSunan Gunung Djati State Islamic University Bandung1
Frida Aziz DamayantiSunan Gunung Djati State Islamic University Bandung1
Fiona Putri SeptianiSunan Gunung Djati State Islamic University Bandung1
Agung WahanaSunan Gunung Djati State Islamic University Bandung1
Lela Sri WahyuniSunan Gunung Djati State Islamic University Bandung1
Fitrahuddin AssidiqSunan Gunung Djati State Islamic University Bandung1
Neng MastianiSunan Gunung Djati State Islamic University Bandung1
Nurul TafianiSunan Gunung Djati State Islamic University Bandung1
Anggita Rahmi HafsariSunan Gunung Djati State Islamic University Bandung1
Nita Puspita TazkiahSunan Gunung Djati State Islamic University Bandung1
Lina YuliantiSunan Gunung Djati State Islamic University Bandung1
Hany HandayaniSunan Gunung Djati State Islamic University Bandung1
Tety SudiartiSunan Gunung Djati State Islamic University Bandung1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.uinjkt.ac.id/index.php/valensi/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Isolation and Identification of Lactic Acid Bacteria form Chinese Cabbage Waste by 16s rRNA Amplification using The Polymerase Chain Reaction (PCR) Method</dc:title>
	<dc:creator xml:lang="en">Junitasari, Assyifa</dc:creator>
	<dc:creator xml:lang="en">Rosahdi, Tina Dewi</dc:creator>
	<dc:creator xml:lang="en">Lestari, Yuni Siti</dc:creator>
	<dc:subject xml:lang="en">Lactic acid bacteria</dc:subject>
	<dc:subject xml:lang="en">16s rRNA gene fragment</dc:subject>
	<dc:subject xml:lang="en">PCR</dc:subject>
	<dc:subject xml:lang="en">gram staining</dc:subject>
	<dc:subject xml:lang="en">sequencing</dc:subject>
	<dc:description xml:lang="en">Waste is material that is disposed of from sources of human and natural activities that do not yet have economic value. Physically, vegetable waste is perishable due to its high-water content, especially Chinese Cabbage waste. The purpose of this study was the isolation and identification of lactic acid bacteria from Chinese Cabbage waste. Isolation was carried out to obtain isolates of lactic acid bacteria from Chinese Cabbage waste. Then it was amplified using a Polymerase Chain Reaction (PCR) instrument. The results of chromosomal DNA amplification of bacterial isolates from mustard 1, mustard 2 and mustard 3 waste showed DNA bands with a size of ± 1200 bp, so it can be concluded that the process of amplification of the 16s rRNA gene fragment in mustard 1, mustard 2 and mustard 3 isolates was successful. The results of the 16s rRNA base sequence analysis showed that the mustard 1 isolate had a similarity index of 84.65% with Bacillus sp, the gene for the mustard 2 isolate had a similarity index of 84.09% with the Uncultured bacterium clone, and the mustard 3 isolate gene had a similarity index of 85.42% with Environmental 16s rDNA sequence.</dc:description>
	<dc:publisher xml:lang="en">Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University</dc:publisher>
	<dc:date>2023-06-04</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinjkt.ac.id/index.php/valensi/article/view/29466</dc:identifier>
	<dc:identifier>10.15408/jkv.v9i1.29466</dc:identifier>
	<dc:source xml:lang="en">Jurnal Kimia Valensi; Jurnal Kimia VALENSI Volume 9, No. 1, May 2023; 136-144</dc:source>
	<dc:source>2548-3013</dc:source>
	<dc:source>2460-6065</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinjkt.ac.id/index.php/valensi/article/view/29466/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Assyifa Junitasari</dc:rights>
</oai_dc:dc>