Jurnal SINTA 1
Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS)
VALENSI · Vol. ume No. 2 (2023)
- ISSN
- 2548-3013, 2460-6065
- Terbit
- 2023-12-28
- Penerbit
- Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
- Bahasa
- eng
Abstrak
Soil bacteria from tropical environments play a significant role in resolving various environmental issues, including biodegradation. Exploratory research on biodiversity is crucial to develop and harness the potential of different types of soil bacteria that are highly abundant. The bacterial diversity in landfills is typically high due to the decomposition of organic and inorganic waste, creating a favorable medium for the growth and development of soil bacteria. This study aims to assess the candidates of xenobiotic degrading indigenous bacteria from the Probolinggo City landfill using Next Generation Sequencing (NGS) method. The research stages include: 1) sampling, 2) isolation of genomic DNA from samples using the ZymoBIOMICS DNA MiniPrep Kit from Zymo Research, 3) amplification of isolated DNA with primers 16S 27F – 1429R, 4) sequencing the results of DNA amplification with NGS, 5) downstream analysis of the results using software Pavian Krona Tools, and 6) narrative analysis review to identify the candidates of xenobiotic degrading indigenous bacteria. The results show that soil samples from the Probolinggo City landfill exhibited a high diversity of bacterial communities. Based on NGS analysis, 2400 bacterial species were identified, comprising 56 genera, 17 orders, 4 classes, and 4 phyla, with respective abundances of Proteobacteria (70%), Firmicutes (15%), Planctomycetes (2%), and Cyanobacteria (0,3%). Based on the narrative analysis review, several bacteria in the Probolinggo City landfill exhibited potential as: 1) polypropylene-degrading bacteria, including Bacillus cereus, B. licheniformis and B. thuringiensis. 2) styrofoam degrading bacteria, namely Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus and Pseudomonas aeruginosa. 3) total ammonia nitrogen (TAN) reducing bacteria, including Bacillus megaterium. 4) pesticide degrading bacteria Profenofos and Chlorantraniliprole, including Bacillus stearothermophilus. and 5) tannic acid degrading bacteria, including Pantoea dispersa. These results indicate that the Probolinggo City landfill is a good habitat for various xenobiotic-degrading bacteria, then the isolation of specific bacteria can be designed using an appropriate selective medium.
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- State University of Malang
- University of Technology Malaysia
- International Islamic University Malaysia
- The Federal Polytechnic, Ado-Ekiti
- State University of Semarang
- Asian Institute of Technology
- University of Brawijaya
- Organisasi lain
- Afiliasi tidak diketahui
- Organisasi berbeda
- Organisasi sama
- Afiliasi tidak diketahui
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Tampilkan sebagai tabel
| Rekan penulis | Organisasi | Artikel bersama |
|---|---|---|
| Fahrul Huyop | University of Technology Malaysia | 4 |
| Habeebat Adekilekun Oyewusi | The Federal Polytechnic, Ado-Ekiti | 2 |
| Sunarmi Sunarmi | State University of Semarang | 1 |
| Elhah Nailul Khasna | State University of Malang | 1 |
| Dwi Inne Listyorini | State University of Malang | 1 |
| Suharti Suharti | State University of Malang | 1 |
| Dyati Galuh Pratita | State University of Malang | 1 |
| Tita Putri Milasari | State University of Malang | 1 |
| Delia Wahyu Pangesti | State University of Malang | 1 |
| Annasa Sabatia | State University of Malang | 1 |
| Syamsul Bachri | State University of Malang | 1 |
| Siti Sendari | State University of Malang | 1 |
| Listyo Yudha Irawan | State University of Malang | 1 |
| Rajendra Prasad SHRESTHA | Asian Institute of Technology | 1 |
| Egi Nursari Billah | State University of Malang | 1 |
| Nanda Regita Cahyaning Putri | State University of Malang | 1 |
| Mellinia Regina Heni Prastiwi | State University of Malang | 1 |
| A. Riyan Rahman Hakiki | State University of Malang | 1 |
| Tabita May Hidiyah | State University of Malang | 1 |
| Armaini Armaini | Andalas University | 1 |
| Evy Damayanthi | IPB University | 1 |
| Muntholib Muntholib | State University of Malang | 1 |
| Aman Santoso | State University of Malang | 1 |
| Lilik Eka Radiati | University of Brawijaya | 1 |
| Amiroh NABILAH-MUJAHIDAH | State University of Malang | 1 |
| Muhammad Asrori | State University of Malang | 1 |
| Yilmaz Kaya | Afiliasi tidak diketahui | 1 |
| Azzmer Azzar Abdul Hamid | International Islamic University Malaysia | 1 |
| Batool Hazim Abdul Wahhab | Mustansiriyah University | 1 |
| Nurul Fatin Syamimi Khairul Anuar | University of Technology Malaysia | 1 |
| Marwan S.M. Al Nimer | Hawler Medical University | 1 |
| Nurul Hidayah Samsulrizal | International Islamic University Malaysia | 1 |
| Mohamed Faraj Edbeib | Bani-Waleed University | 1 |
| Muhammad Muhammad | Federal University Dutse | 1 |
| Muhammad Muhammad | Malikussaleh University | 1 |
| Mohd Hefni Rusli | Malaysian Palm Oil Board | 1 |
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