Jurnal SINTA 1
Anticancer Activities of Bromelain Hydrolysate of Soy Protein Against Breast Cancer Cells MCF-7
VALENSI · Vol. ume No. 1 (2024) · hlm. 97–105
- ISSN
- 2548-3013, 2460-6065
- Terbit
- 2024-06-09
- Penerbit
- Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
- Bahasa
- eng
Abstrak
Soybeans contain proteins that have the potential to produce anticancer bioactive peptides. This study aims to determine the anticancer activity of soy protein hydrolysate against MCF-7 breast cancer cells. Soybean protein hydrolyzed by bromelain enzyme 0.5% (w/v) at the optimum temperature and pH for protein hydrolysis using the Bergmeyer and Grassl method. The degree of hydrolysis value of protein hydrolysate was determined by the Alder-Niesen method and the protein profile was analyzed by SDS-PAGE. The hydrolysate with the best degree of hydrolysis value was analyzed for anticancer activity against MCF-7 breast cancer cells by the Presto Blue assay method, and fractionation of protein hydrolysates by gel filtration chromatography (Sephadex G-15). The molecular weight of the peptide was characterized by LCMS/MS. Soy protein hydrolysis using 0.5% (w/v) bromelain enzyme was optimum at 65 ºC and pH 7.0 for 4 hours, with a hydrolysis degree value of 20.57%. The SDS-PAGE analysis showed that the protein hydrolysates had quite thick protein bands in the range of <35 kDa with an IC50 value of 70.37 mg/mL. Based on the LCMS/MS results, the peptide from fractionation has a molecular weight of 5.133 kDa.
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40 penulis · 112 tautan kepenulisan bersama · 54 lintas organisasi · menampilkan 40 yang paling banyak terhubung
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- Syarif Hidayatullah State Islamic University Jakarta
- National Nuclear Energy Agency of Indonesia
- Bandung Institute of Technology
- Universitas Mathla'ul Anwar Banten
- Sunan Kalijaga State Islamic University Yogyakarta
- University of Indonesia
- Indonesia Defense University
- Organisasi lain
- Organisasi berbeda
- Organisasi sama
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Tampilkan sebagai tabel
| Rekan penulis | Organisasi | Artikel bersama |
|---|---|---|
| Dede Sukandar | Syarif Hidayatullah State Islamic University Jakarta | 7 |
| Siti Nurbayti | Syarif Hidayatullah State Islamic University Jakarta | 4 |
| Irawan Sugoro | National Nuclear Energy Agency of Indonesia | 3 |
| Ahmad Fathoni | Syarif Hidayatullah State Islamic University Jakarta | 3 |
| Sri Yadial Chalid | Syarif Hidayatullah State Islamic University Jakarta | 3 |
| Anna Muawanah | Syarif Hidayatullah State Islamic University Jakarta | 2 |
| Asih Widi Wisudawati | Sunan Kalijaga State Islamic University Yogyakarta | 2 |
| Muhammad Ihda H.L. Zein | Syarif Hidayatullah State Islamic University Jakarta | 2 |
| Barita Juliano Siregar | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Denny Alfarus | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Abdul Mutalib | Padjadjaran University | 1 |
| Yahdiana Harahap | Indonesia Defense University | 1 |
| Sunarhadijoso Soenarjo | National Nuclear Energy Agency of Indonesia | 1 |
| Ratna Dini Haryuni | National Nuclear Energy Agency of Indonesia | 1 |
| Anton Bahtiar | University of Indonesia | 1 |
| Martalena Ramli | National Nuclear Energy Agency of Indonesia | 1 |
| C.N. Ardiyatno | National Nuclear Energy Agency of Indonesia | 1 |
| Veronika Yulianti Susilo | National Nuclear Energy Agency of Indonesia | 1 |
| D. Haffid | National Nuclear Energy Agency of Indonesia | 1 |
| Ikmalia Ikmalia | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Emi Lestari | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Rizkina Harahap | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Mirzan Thabrani Razzak | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Priyambodo Priyambodo | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Septyani Nurichawati | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Pingkan Aditiawati | Bandung Institute of Technology | 1 |
| Dwiwahju Sasongko | Bandung Institute of Technology | 1 |
| Dea Rizky Indriani | Bandung Institute of Technology | 1 |
| ROBIATUL ADAWIYAH | Universitas Nurul Jadid | 1 |
| Prita Wardhani | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Imamah Al Mabrur | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Cut Dhien K Meutia | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Hilman Affandi | Southeast Asian Regional Centre for Tropical Biology | 1 |
| Thamzil Las | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Farhat Etriya | Syarif Hidayatullah State Islamic University Jakarta | 1 |
| Eddy Jusuf | Indonesian Institute of Sciences | 1 |
| M. Hero Shiddiqi | Syarif Hidayatullah State Islamic University Jakarta | 1 |
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