Akses terbuka Jurnal SINTA 1
Efficacy Test of Prototype Kit for Detection Bacillus cereus and Listeria monocytogenes in Processed Meat using Real-time PCR Method
VALENSI · Vol. ume No. 1 (2025) · hlm. 50–59
- ISSN
- 2548-3013, 2460-6065
- Terbit
- 2025-05-31
- Penerbit
- Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
- Bahasa
- eng
Abstrak
According to the World Health Organization (WHO), harmful agents such as Bacillus cereus and Listeria monocytogenes are responsible for 600 million cases of disease and 420,000 deaths annually. This research aims to test the effectiveness of the real-time PCR method for developing a prototype kit to detect pathogenic bacteria in processed meat. As a comparison, the and conventional PCR methods were used to obtain the accuracy, specificity, sensitivity, and effectiveness of the real-time PCR method. All the samples were cultured in solid media agar, performed amplification using specific primers cyt-K 2 and hly using PCR and real-time PCR. Meatballs, nuggets, and sausages, five samples each, were found to be positive positively contaminated with all targeted bacteria. However, they did not provide specific results using solid media culture and the PCR method. In addition, the real-time PCR method using prototype kit formulas accomplished that all contaminated samples had a Ct value smaller than the negative control, NTC (No Template Control), and had a similar melting curve to the positive control. This establishes that the real-time PCR method clarifies that all samples were contaminated with target bacteria. A formula was developed in the prototype kit with real-time PCR methods that can be used and applied on a commercial scale efficiently and precisely.
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- State University of Jakarta
- Arnold Palmer Hospital for Children
- Bandung Institute of Technology
- Universitas Airlangga
- City of Scientific Research and Technological Applications
- Chulalongkorn University
- Indonesia University of Education
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Tampilkan sebagai tabel
| Rekan penulis | Organisasi | Artikel bersama |
|---|---|---|
| Bassam Abomoelak | Arnold Palmer Hospital for Children | 35 |
| Dandy Akbar Juliansyah | State University of Jakarta | 32 |
| Gusti Angieta Putri | State University of Jakarta | 19 |
| Sri Rahayu | University of Brawijaya | 13 |
| Rosita GIo Anggraeni | State University of Jakarta | 13 |
| Anisa Fitriyanti | State University of Jakarta | 11 |
| Ade Danova | Bandung Institute of Technology | 5 |
| Elvira Hermawati | Bandung Institute of Technology | 5 |
| Novitasari | Afiliasi tidak diketahui | 5 |
| Rara Seruni | State University of Jakarta | 4 |
| Iqbal Musthapa | Indonesia University of Education | 2 |
| Warinthorn Chavasiri | Chulalongkorn University | 2 |
| Futi Kusuma Hati | State University of Jakarta | 2 |
| Nur Fadillah Adelia Azahra | State University of Jakarta | 2 |
| Fadhila Shofi Albana | State University of Jakarta | 2 |
| Afifah Aisyafitri | State University of Jakarta | 2 |
| Dema Griseldis Aurellia | State University of Jakarta | 2 |
| Siti Munawaoh | State University of Jakarta | 2 |
| Siti Munawaroh | State University of Jakarta | 2 |
| Tri Susanti | State University of Jakarta | 2 |
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