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Isolation, Characterization, and Identification of Root-Associated Endophytic Bacteria Producing Chitinase from Strawberry Plants

VALENSI · Vol. ume No. 1 (2026) · hlm. 14–25

DOI
10.15408/jkv.v12i1.46618
ISSN
2548-3013, 2460-6065
Terbit
2026-05-31
Penerbit
Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

Endophytic bacteria inhabiting root tissues represent a promising resource for biotechnological applications, particularly as chitinase producers for biological control. This study aimed to isolate, characterize, and identify chitinase-producing endophytic bacteria from strawberry (Fragaria spp.) roots. Root samples underwent a surface sterilization process and serial dilutions before being planted on selective media with a chitin colloidal substrate. They were then characterized morphologically and identified molecularly through analysis of the 16S rRNA gene. The results of the initial screening using agar medium with chitin colloidal substrate at a temperature of 37 °C yielded four bacterial isolates. Two of the bacterial isolates showed high chitinolytic index (CI), where isolate RS-A1 had a CI (1.55) and isolate RS-A2 had a CI (1.65). The electrophoresis of the 16S rRNA PCR product showed an amplicon of approximately 1300 bp. BLAST analysis against GenBank showed that isolate RS-A1 had 98.06% similarity to Acinetobacter baumannii, whereas isolate RS-A2 had 97.21% similarity to Bacillus aerius. The quantitative result of specific chitinase activity was highest in the RS-A2 isolate at 0.01146 U/mg, while that of the RS-A1 isolate was 0.00781 U/mg. These results indicate that endophytic bacteria from strawberry plant roots have the potential to act as biocontrol agents.

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Seniwati DaliAfiliasi tidak diketahui10
Alfian NoorHasanuddin University9
Paulina TabaHasanuddin University7
ABDUL KARIMAfiliasi tidak diketahui5
Siti KhairunnurAfiliasi tidak diketahui5
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Ajuk SaparAfiliasi tidak diketahui3
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La KoloAfiliasi tidak diketahui2
Djabal Nur BasirHasanuddin University2
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Maming MamingAfiliasi tidak diketahui2
Muhammad AbasUniversitas Halu Oleo1
Abdur Rahman ArifAfiliasi tidak diketahui1
Ischaidar IschaidarAfiliasi tidak diketahui1
Suhaela SuhaelaTadulako University1
Erna MayasariAfiliasi tidak diketahui1
Mahdalia MahmudAfiliasi tidak diketahui1
Eva JohannesAfiliasi tidak diketahui1
Tjodi HarlimAfiliasi tidak diketahui1
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.uinjkt.ac.id/index.php/valensi/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Isolation, Characterization, and Identification of Root-Associated Endophytic Bacteria Producing Chitinase from Strawberry Plants</dc:title>
	<dc:creator xml:lang="en">Aulia, Nur </dc:creator>
	<dc:creator xml:lang="en">Natsir, Hasnah </dc:creator>
	<dc:creator xml:lang="en">Ahmad, Ahyar </dc:creator>
	<dc:creator xml:lang="en">Soekamto, Nunuk Hariani</dc:creator>
	<dc:creator xml:lang="en">Wahab, Abd. Wahid </dc:creator>
	<dc:creator xml:lang="en">Arfah, Rugaiyah Andi</dc:creator>
	<dc:creator xml:lang="en">Karim, Harningsih </dc:creator>
	<dc:subject xml:lang="en">Acinetobacter baumannii</dc:subject>
	<dc:subject xml:lang="en">Bacillus aerius</dc:subject>
	<dc:subject xml:lang="en">chitinase</dc:subject>
	<dc:subject xml:lang="en">endophytic bacteria</dc:subject>
	<dc:description xml:lang="en">Endophytic bacteria inhabiting root tissues represent a promising resource for biotechnological applications, particularly as chitinase producers for biological control. This study aimed to isolate, characterize, and identify chitinase-producing endophytic bacteria from strawberry (Fragaria spp.) roots. Root samples underwent a surface sterilization process and serial dilutions before being planted on selective media with a chitin colloidal substrate. They were then characterized morphologically and identified molecularly through analysis of the 16S rRNA gene. The results of the initial screening using agar medium with chitin colloidal substrate at a temperature of 37 °C yielded four bacterial isolates. Two of the bacterial isolates showed high chitinolytic index (CI), where isolate RS-A1 had a CI (1.55) and isolate RS-A2 had a CI (1.65). The electrophoresis of the 16S rRNA PCR product showed an amplicon of approximately 1300 bp. BLAST analysis against GenBank showed that isolate RS-A1 had 98.06% similarity to Acinetobacter baumannii, whereas isolate RS-A2 had 97.21% similarity to Bacillus aerius. The quantitative result of specific chitinase activity was highest in the RS-A2 isolate at 0.01146 U/mg, while that of the RS-A1 isolate was 0.00781 U/mg. These results indicate that endophytic bacteria from strawberry plant roots have the potential to act as biocontrol agents. </dc:description>
	<dc:publisher xml:lang="en">Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University</dc:publisher>
	<dc:date>2026-05-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinjkt.ac.id/index.php/valensi/article/view/46618</dc:identifier>
	<dc:identifier>10.15408/jkv.v12i1.46618</dc:identifier>
	<dc:source xml:lang="en">Jurnal Kimia Valensi; Jurnal Kimia VALENSI,  Volume 12, No. 1, May 2026; 14-25</dc:source>
	<dc:source>2548-3013</dc:source>
	<dc:source>2460-6065</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinjkt.ac.id/index.php/valensi/article/view/46618/19245</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Nur  Aulia, Hasnah  Natsir, Ahyar  Ahmad, Nunuk Hariani Soekamto, Abd. Wahid  Wahab, Rugaiyah Andi Arfah, Harningsih  Karim</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>