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Kinetic Behavior and Stability of Alginate-Immobilized Urease from Green Bean Seeds (Phaseolus vulgaris)

VALENSI · Vol. ume No. 1 (2026) · hlm. 86–96

DOI
10.15408/jkv.v12i1.49961
ISSN
2548-3013, 2460-6065
Terbit
2026-05-31
Penerbit
Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-sa/4.0

Abstrak

The industrial application of urease is limited due to its low reusability, which can be overcome by immobilization. Furthermore, the utilization of urease from plants such as mung bean seeds, which is affordable and readily available, offers a sustainable alternative to commercial enzymes. This study aimed to immobilize urease extracted from green bean seeds using an alginate matrix and to evaluate its kinetic and stability characteristics. Urease activity was measured at 500 nm using the Nessler method. Kinetic analysis showed that immobilization decreased Vmax from 0.674 to 0.223 M min-1 and increased KM from 0.005169 to 0.006755 M. After incubation at 35 oC for 180 min, immobilized urease showed higher relative activity (52.26%) than free enzyme (42.82%). Stored at 4 oC for 9 days, the enzyme preserved 56.68% of its relative activity. The immobilized urease displayed the ability to be reused up to five cycles. SEM analysis showed morphological changes in the beads after enzyme incorporation, while EDX confirmed an increase in the elemental composition of the immobilized beads. This study shows that urease from green bean seeds can be effectively immobilized in an alginate matrix to produce a stable, reusable, low-cost biocatalyst with potential for application in urea biosensors.

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Aprilia Nafi NurainiJenderal Soedirman University4
Puji LestariJenderal Soedirman University4
Uyi SulaemanJenderal Soedirman University3
Aris HaryadiJenderal Soedirman University2
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I Made ArtikaIPB University1
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Salfa Athallah Agtari NabillahNational Research and Innovation Agency1
Roidah Cahya WatiJenderal Soedirman University1
Siti NurmilahPadjadjaran University1
Andri FrediansyahNational Research and Innovation Agency1
A. ArifinHasanuddin University1
Jamrud AminuddinJenderal Soedirman University1
Nurhayati NurhayatiUniversitas Islam Negeri Ar-Raniry1
Agustina WidiyaniSumatera Institute of Technology1
Mukhtar EffendiJenderal Soedirman University1
Abdullah Nur AzizJenderal Soedirman University1
Pakhrur RaziUniversitas Negeri Padang1
Wihantoro WihantoroJenderal Soedirman University1
Farzand AbdullatifJenderal Soedirman University1
Sunardi SunardiJenderal Soedirman University1
Yohannes SardjonoNational Nuclear Energy Agency of Indonesia1
Yaser KasesazNuclear Science and Technology Research Institute1
Khansa SalsabilaSebelas Maret University1
Dadan HermawanJenderal Soedirman University1
Mudasir MudasirUniversitas Gadjah Mada1
Ponco IswantoJenderal Soedirman University1
Cacu CacuJenderal Soedirman University1
Suwandri SuwandriJenderal Soedirman University1
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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.uinjkt.ac.id/index.php/valensi/oai, pertama di-harvest 10 Nov 2026, terakhir terlihat 10 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Kinetic Behavior and Stability of Alginate-Immobilized Urease from Green Bean Seeds (Phaseolus vulgaris)</dc:title>
	<dc:creator xml:lang="en">Zusfahair, Zusfahair</dc:creator>
	<dc:creator xml:lang="en">Setiawan, Sifa Nurfadillah </dc:creator>
	<dc:creator xml:lang="en">Ningsih, Dian Riana</dc:creator>
	<dc:creator xml:lang="en">Bilalodin, Bilalodin</dc:creator>
	<dc:creator xml:lang="en">Fatoni, Amin </dc:creator>
	<dc:creator xml:lang="en">Istikhari Muslihah, Niken </dc:creator>
	<dc:subject xml:lang="en">Alginate</dc:subject>
	<dc:subject xml:lang="en">green bean seeds</dc:subject>
	<dc:subject xml:lang="en">immobilization</dc:subject>
	<dc:subject xml:lang="en">urease</dc:subject>
	<dc:description xml:lang="en">The industrial application of urease is limited due to its low reusability, which can be overcome by immobilization. Furthermore, the utilization of urease from plants such as mung bean seeds, which is affordable and readily available, offers a sustainable alternative to commercial enzymes. This study aimed to immobilize urease extracted from green bean seeds using an alginate matrix and to evaluate its kinetic and stability characteristics. Urease activity was measured at 500 nm using the Nessler method. Kinetic analysis showed that immobilization decreased  Vmax from 0.674 to 0.223 M min-1 and increased KM from 0.005169 to 0.006755 M. After incubation at 35 oC for 180 min, immobilized urease showed higher relative activity (52.26%) than free enzyme (42.82%). Stored at 4 oC for 9 days, the enzyme preserved 56.68% of its relative activity. The immobilized urease displayed the ability to be reused up to five cycles. SEM analysis showed morphological changes in the beads after enzyme incorporation, while EDX confirmed an increase in the elemental composition of the immobilized beads. This study shows that urease from green bean seeds can be effectively immobilized in an alginate matrix to produce a stable, reusable, low-cost biocatalyst with potential for application in urea biosensors.</dc:description>
	<dc:publisher xml:lang="en">Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University</dc:publisher>
	<dc:date>2026-05-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.uinjkt.ac.id/index.php/valensi/article/view/49961</dc:identifier>
	<dc:identifier>10.15408/jkv.v12i1.49961</dc:identifier>
	<dc:source xml:lang="en">Jurnal Kimia Valensi; Jurnal Kimia VALENSI,  Volume 12, No. 1, May 2026; 86-96</dc:source>
	<dc:source>2548-3013</dc:source>
	<dc:source>2460-6065</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.uinjkt.ac.id/index.php/valensi/article/view/49961/19251</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Zusfahair Zusfahair, Sifa Nurfadillah  Setiawan, Dian Riana Ningsih, Bilalodin Bilalodin, Amin  Fatoni, Niken  Istikhari Muslihah</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>