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Spermatozoa Viability of Filial Ettawa Goat After Sexing Process

Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY (2016)

Published
2016-07-13
Publisher
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY
Language
eng

Abstract

The use of albumin derived from egg white is a method that is easy to apply, cheap, effective for sexing semen process of separating the spermatozoa X and Y. The viability of Filial Ettawa goatspermatozoa after the sexing process using different percentages of albumin density egg white withcoconut water diluent and egg yolk diluents was examined. The egg white albumin densitytreatments were 3 gradients of 10%, 30% and 50%. Laboratory experiments were performed sexingspermatozoa using the 3 density gradients of egg white albumin with coconut water diluent and eggyolks diluents with a 20 minute incubation time. Each treatment was replicated 10 times. Data wasstatistically analyzed using analysis of variance model (ANOVA). The addition of coconut waterdiluent and egg yolk diluent provide a highly significant difference (p0.01) in the spermatozoaseparation of the top and bottom layers, but the average value there is no significant difference. Thespermatozoa viability after sperm sexing found that using density gradient egg whites showed betterresults using egg yolk diluent compared to coconut water diluent. The top layer had a mean percentlive sperm of 78.52 ± 8.65% and the bottom layer had mean percent live sperm of 80.29 ± 8.735%.Further research is needed into the quality of spermatozoa sexing results using varying gradients ofegg whites.Key Words: Sexing, Egg White Albumin Density, Diluent, Egg Yolk, Goat

Collaboration network of the authors

23 authors · 74 co-authorship links · 5 across different organisations

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  • University of Brawijaya
  • Politeknik Pertanian Negeri Samarinda
  • Universitas Kanjuruhan
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Kuswati KuswatiAffiliation unknown5
Aulia Puspita Anugra YektiUniversity of Brawijaya5
Muchamad LuthfiAffiliation unknown3
Asri Nurul HudaUniversity of Brawijaya2
Dedi MuhammadUniversity of Brawijaya2
Lukman Affandhy SunartoAffiliation unknown2
Muhammad Ade SalimUniversity of Brawijaya2
Muhammad Nur IhsanUniversity of Brawijaya2
Noni Ashri MaghfirohAffiliation unknown1
Wike Andre SeptianAffiliation unknown1
Dony HerviyantoAffiliation unknown1
Willy Saputra Saputra TatulusPoliteknik Pertanian Negeri Samarinda1
Dian RatnawatiUniversity of Brawijaya1
L AffandhyUniversity of Brawijaya1
Brilla Ismaya SafitriAffiliation unknown1
Sri WahjuningsihAffiliation unknown1
Jois HarsahAffiliation unknown1
Muhammad DikmanAffiliation unknown1
Enike Dwi KusumawatiUniversitas Kanjuruhan1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/P-ISITAPFFS/oai, first harvested 30 Okt 2026, last seen 1 Nov 2026.
Original metadata (oai_dc)
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	<dc:title xml:lang="en-US">Spermatozoa Viability of Filial Ettawa Goat After Sexing Process</dc:title>
	<dc:creator>Kusumawati, Enik Dwi</dc:creator>
	<dc:creator>Leondro, Henny</dc:creator>
	<dc:creator>Susilawati, Trinil</dc:creator>
	<dc:creator>Isnaini, Nurul</dc:creator>
	<dc:description xml:lang="en-US">The use of albumin derived from egg white is a method that is easy to apply, cheap, effective for sexing semen process of separating the spermatozoa X and Y. The viability of Filial Ettawa goatspermatozoa after the sexing process using different percentages of albumin density egg white withcoconut water diluent and egg yolk diluents was examined. The egg white albumin densitytreatments were 3 gradients of 10%, 30% and 50%. Laboratory experiments were performed sexingspermatozoa using the 3 density gradients of egg white albumin with coconut water diluent and eggyolks diluents with a 20 minute incubation time. Each treatment was replicated 10 times. Data wasstatistically analyzed using analysis of variance model (ANOVA). The addition of coconut waterdiluent and egg yolk diluent provide a highly significant difference (p0.01) in the spermatozoaseparation of the top and bottom layers, but the average value there is no significant difference. Thespermatozoa viability after sperm sexing found that using density gradient egg whites showed betterresults using egg yolk diluent compared to coconut water diluent. The top layer had a mean percentlive sperm of 78.52 ± 8.65% and the bottom layer had mean percent live sperm of 80.29 ± 8.735%.Further research is needed into the  quality of spermatozoa sexing results using varying gradients ofegg whites.Key Words: Sexing, Egg White Albumin Density, Diluent, Egg Yolk, Goat</dc:description>
	<dc:publisher xml:lang="en-US">Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2016-07-13</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/P-ISITAPFFS/article/view/1138</dc:identifier>
	<dc:source xml:lang="en-US">Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY; PROCEEDING INTERNATIONAL SEMINAR</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/P-ISITAPFFS/article/view/1138/785</dc:relation>
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