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Karakteristik Segmen Gen sitokrom C Oksidase Subunit I (COI) Ngengat Plusia chalcites (Lepidoptera: Noctuidae)

BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) · Vol. 6 No. 2 (2019) · pp. 985–994

DOI
10.33772/biowallacea.v6i2.8824
ISSN
2685-6360, 2355-6404
Published
2019-11-06
Publisher
University of Halu Oleo
Language
eng
License
creativecommons.org/licenses/by/4.0

Abstract

ABSTRACTThe research was exploration ofpartial COI gene of moth (Plusia chalcites).The aim of this research is to characterization of nucleotide sequence of these COI gene, that was exracted with Cetyl Trimetyl Ammonium Bromide (CTAB) method, and then amplified by Polymerase Chain Reaction (PCR) technique with spesifik primer. The result PCR amplified subsequent analysis of nitrogen base arrangement in these COI gene. The analysis data using the BioEdit and MEGA Version 5.0 software.The results showed that the segment of COI gene in Plusia chalcites as long 710 bp (base pairs). From these, there are 503/657 nucleotides which are conserved, 154/657 nucleotides of varies, 124/657 parsimoni informative nucleotides and 30/657 singeleton nucleotides. The base N composition consist of 39.0% Timine, 14.0% Cytosin (C), 31.7%Adenine (A) and 15.4% Guanin.Key words: Plusia chalcites, COI Gene characteristics and composition. ABSTRAK Penelitian ini merupakan penelitian eksplorasi untuk mengkarakterisasi sekuen gen COI. Karakterisasi gen COI meliputi isolasi DNA menggunakan metode CTAB (Cetyl Trimetyl Ammonium Bromide), amplifikasi gen COI dengan teknik PCR (Polymerase Chain Reaction) dan squencing untuk analisis susunan basa nitrogen pada gen COI. Analisis data molekuler menggunakan software BioEdit dan MEGA Version 5.0.Hasil penelitian menunjukkan segmen gen COI Plusia chalcites yang teramplifikasi berukuran 710 pb, dan terkarakterisasi sepanjang 657pb. Dari segmen tersebut terdapat 503/657 nukleotida yang bersifat conserved (kekal), 154/657 nukleotida yang bersifat variabel (bervariasi), 124/657 nukleotida parsimoni informative dan 30/657 nukleotida singleton. Komposisi basa nitrogen sekuen gen COI Plusia chalcites yaitu Timin (T) 39,0%, Cytosin (C) 14.0%, Adenin (A) 31,7% dan Guanin 15,4%..Kata kunci:Plusia chalcites, Karakteristik Gen COI

Keywords

Collaboration network of the authors

40 authors · 144 co-authorship links · 3 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • State University of Medan
  • Universitas Sulawesi Tenggara
  • Universitas Gadjah Mada
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Nur Isiyana WiantiAffiliation unknown4
Sukmawati AbdullahAffiliation unknown3
Muslim TadjuddahAffiliation unknown3
Tjandra BuanaAffiliation unknown3
Yani TaufikAffiliation unknown3
Ima Astuty WunawarsihAffiliation unknown3
La NalefoAffiliation unknown2
Awaluddin HamzahUniversitas Halu Oleo2
Muzuni MuzuniAffiliation unknown2
Jahidin JahidinAffiliation unknown2
Taane La OlaAffiliation unknown2
Mardin MardinAffiliation unknown2
Bunyamin BunyaminAffiliation unknown2
Muhammad AminState University of Medan1
Wulan Ali RahminAffiliation unknown1
Fitriani FitrianiAffiliation unknown1
Musadar MappasombaAffiliation unknown1
Mita MitaUniversitas Sulawesi Tenggara1
Hartina BatoaAffiliation unknown1
Rifka TaufikaAffiliation unknown1
Muh. Yusril LaoteAffiliation unknown1
Rosmawaty RosmawatyAffiliation unknown1
Muh. ArisantoAffiliation unknown1
Musadar MusadarAffiliation unknown1
Nurfadilla NurfadillaAffiliation unknown1
Alias AliasAffiliation unknown1
Suci Cahaya RezkiAffiliation unknown1
La HasaniAffiliation unknown1
Arni ArniUniversitas Halu Oleo1
Muhammad Aswar LimiUniversitas Halu Oleo1
Putu ArimbawaAffiliation unknown1
Monalisa MonalisaAffiliation unknown1
Salahuddin SalahuddinUniversitas Gadjah Mada1
Nurhayu MalikAffiliation unknown1
Victor BragancaAffiliation unknown1
La Ode Ali AmaniAffiliation unknown1
La SarifudinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/wallacea/oai, first harvested 31 Okt 2026, last seen 31 Okt 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Karakteristik Segmen Gen sitokrom C Oksidase Subunit I (COI) Ngengat Plusia chalcites (Lepidoptera: Noctuidae)</dc:title>
	<dc:creator>Suriana, Suriana</dc:creator>
	<dc:creator>Marwansyah, Marwansyah</dc:creator>
	<dc:creator>Amirullah, Amirullah</dc:creator>
	<dc:subject xml:lang="en-US">Genetic and Biomolecular</dc:subject>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">ABSTRACTThe research was exploration ofpartial COI gene of moth (Plusia chalcites).The aim of this research is to characterization of nucleotide sequence of these COI gene, that was exracted with Cetyl Trimetyl Ammonium Bromide (CTAB) method, and then amplified by Polymerase Chain Reaction (PCR) technique with spesifik primer. The result PCR amplified subsequent analysis of nitrogen base arrangement in these COI gene. The analysis data using the BioEdit and MEGA Version 5.0 software.The results showed that the  segment of COI gene in Plusia chalcites as long 710 bp (base pairs). From these, there are 503/657 nucleotides which are conserved, 154/657 nucleotides of varies, 124/657 parsimoni informative nucleotides and 30/657 singeleton nucleotides. The base N composition consist of 39.0% Timine, 14.0% Cytosin (C), 31.7%Adenine (A) and 15.4% Guanin.Key words: Plusia chalcites, COI Gene characteristics and composition. ABSTRAK Penelitian ini merupakan penelitian eksplorasi untuk mengkarakterisasi sekuen gen COI. Karakterisasi gen COI meliputi isolasi DNA menggunakan metode CTAB (Cetyl Trimetyl Ammonium Bromide), amplifikasi gen COI dengan teknik PCR (Polymerase Chain Reaction) dan squencing untuk analisis susunan basa nitrogen pada gen COI. Analisis data molekuler menggunakan software BioEdit dan MEGA Version 5.0.Hasil penelitian menunjukkan segmen gen COI Plusia chalcites  yang teramplifikasi berukuran 710 pb, dan terkarakterisasi sepanjang 657pb. Dari segmen tersebut terdapat 503/657 nukleotida yang bersifat conserved (kekal), 154/657 nukleotida yang bersifat variabel (bervariasi), 124/657 nukleotida parsimoni informative dan 30/657 nukleotida singleton. Komposisi basa nitrogen sekuen gen COI Plusia chalcites yaitu Timin (T) 39,0%, Cytosin (C) 14.0%, Adenin (A) 31,7% dan Guanin 15,4%.  .Kata kunci :Plusia chalcites, Karakteristik Gen COI</dc:description>
	<dc:publisher xml:lang="en-US">University of Halu Oleo</dc:publisher>
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	<dc:date>2019-11-06</dc:date>
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	<dc:identifier>https://ojs.uho.ac.id/index.php/wallacea/article/view/8824</dc:identifier>
	<dc:identifier>10.33772/biowallacea.v6i2.8824</dc:identifier>
	<dc:source xml:lang="en-US">BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research); Vol 6, No 2 (2019): BioWallacea and Biotechnological Science; 985-994</dc:source>
	<dc:source>2685-6360</dc:source>
	<dc:source>2355-6404</dc:source>
	<dc:source>10.33772/biowallacea.v6i2</dc:source>
	<dc:language>eng</dc:language>
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	<dc:relation>https://ojs.uho.ac.id/index.php/wallacea/article/downloadSuppFile/8824/786</dc:relation>
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	<dc:rights xml:lang="en-US">Copyright (c) 2019 BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
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