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UJI KEPEKAAN KULIT BATANG CEREMAI (Phyllanthus acidus L.) TERHADAP PERTUMBUHAN Escherichia coli

BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) · Vol. 3 No. 2 (2016)

DOI
10.33772/biowallacea.v3i2.5851
ISSN
2685-6360, 2355-6404
Published
2016-10-11
Publisher
University of Halu Oleo
Language
eng

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui kepekaan kulit batang ceremai (phyllanthus acidus L.) dan untuk mengukur diameter zona hambat kulit batang ceremai terhadap pertumbuhan Escherichia coli ATCC 35218. Sampel dalam penelitian ini adalah kulit batang ceremai yang diekstraksi dengan metode maserasi menggunakan etanol 96%. Dibuat pengenceran dengan konsentrasi 20%, 40%, 60%, 80% dan 100%. Pengujian kepekaan menggunakan media NA dengan metode cawan kertas. Biakan bakteri dari media NB dicampur dengan media agar, kemudian cawan kertas diletakkan di atas media agar. Diinkubasi pada suhu 37oC selama 48 jam. Diukur zona hambat menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa kulit batang ceremai konsentrasi 20% tidak dapat menghambat pertumbuhan bakteri Escherichia coli. Pada konsentrasi 40% rata-rata diameter zona hambat 4,03 mm. Pada konsentrasi 60% rata-rata diameter zona hambat 4,36 mm. Pada konsentrasi 80% rata-rata diameter zona hambat 5,56 mm. Dan pada konsentrasi 100% dapat menghambat pertumbuhan bakteri Escherichia coli secara optimal dengan rata-rata diameter zona hambat 7,03 mm. Kata Kunci: Escherichia coli, ceremai, Nutrient Agar ABSTRACTThis study aims to determine the sensitivity of the bark ceremai (Phyllanthus acidus L.) and to measure the diameter of inhibition zone ceremai bark on the growth of Escherichia coli ATCC 35218. The sample in this study is the bark ceremai extracted by maceration method using ethanol 96%. Created dilution with a concentration of 20%, 40%, 60%, 80% and 100%. NA sensitivity testing using media with paper plate method. NB bacterial culture media mixed with agar medium, then the paper cup is placed on an agar medium. Incubated at 37 ° C for 48 hours. Inhibition zone was measured using a caliper. The results show that the research is conducted bark ceremai concentration of 20% could not inhibit the growth of bacteria Escherichia coli. At a concentration of 40% average inhibition zone diameter of 4.03 mm. At a concentration of 60% average inhibition zone diameter of 4.36 mm. At a concentration of 80% average inhibition zone diameter of 5.56 mm. And at 100% concentration to inhibit the growth of bacteria Escherichia coli optimally with an average diameter of 7.03 mm zone of inhibition. Keywords: Escherichia coli, ceremai, Nutrient Agar

Collaboration network of the authors

8 authors · 12 co-authorship links

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  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Susanti SusantiInstitut Agama Islam Negeri Kendari2
Syamsinar SyamsinarAffiliation unknown1
Irnawati IrnawatiAffiliation unknown1
irin aprilin janharAffiliation unknown1
Firdayanti FirdayantiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/wallacea/oai, first harvested 31 Okt 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">UJI KEPEKAAN KULIT BATANG CEREMAI (Phyllanthus acidus L.) TERHADAP PERTUMBUHAN Escherichia coli</dc:title>
	<dc:creator>Sernita, Sernita</dc:creator>
	<dc:creator>Fusvita, Anggriani</dc:creator>
	<dc:creator>Sumarni, Andri Lina</dc:creator>
	<dc:description xml:lang="en-US">ABSTRAKPenelitian ini bertujuan untuk mengetahui kepekaan kulit batang ceremai (phyllanthus acidus L.) dan untuk mengukur diameter zona hambat kulit batang ceremai  terhadap pertumbuhan Escherichia coli ATCC 35218. Sampel dalam penelitian ini adalah kulit batang ceremai yang diekstraksi dengan metode maserasi menggunakan etanol 96%. Dibuat pengenceran dengan konsentrasi 20%, 40%, 60%, 80% dan 100%. Pengujian kepekaan menggunakan media NA dengan metode cawan kertas. Biakan bakteri dari media NB dicampur dengan media agar, kemudian cawan kertas diletakkan di atas media agar. Diinkubasi pada suhu 37oC selama 48 jam. Diukur zona hambat menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa kulit batang ceremai konsentrasi 20% tidak dapat menghambat pertumbuhan bakteri Escherichia coli. Pada konsentrasi 40% rata-rata diameter zona hambat 4,03 mm. Pada konsentrasi 60% rata-rata diameter zona hambat 4,36 mm. Pada konsentrasi 80% rata-rata diameter zona hambat 5,56 mm. Dan pada konsentrasi 100% dapat menghambat pertumbuhan bakteri Escherichia coli secara optimal dengan rata-rata diameter zona hambat 7,03 mm. Kata Kunci : Escherichia coli, ceremai, Nutrient Agar ABSTRACTThis study aims to determine the sensitivity of the bark ceremai (Phyllanthus acidus L.) and to measure the diameter of inhibition zone ceremai bark on the growth of Escherichia coli ATCC 35218. The sample in this study is the bark ceremai extracted by maceration method using ethanol 96%. Created dilution with a concentration of 20%, 40%, 60%, 80% and 100%. NA sensitivity testing using media with paper plate method. NB bacterial culture media mixed with agar medium, then the paper cup is placed on an agar medium. Incubated at 37 ° C for 48 hours. Inhibition zone was measured using a caliper. The results show that the research is conducted bark ceremai concentration of 20% could not inhibit the growth of bacteria Escherichia coli. At a concentration of 40% average inhibition zone diameter of 4.03 mm. At a concentration of 60% average inhibition zone diameter of 4.36 mm. At a concentration of 80% average inhibition zone diameter of 5.56 mm. And at 100% concentration to inhibit the growth of bacteria Escherichia coli optimally with an average diameter of 7.03 mm zone of inhibition. Keywords : Escherichia coli, ceremai, Nutrient Agar</dc:description>
	<dc:publisher xml:lang="en-US">University of Halu Oleo</dc:publisher>
	<dc:contributor xml:lang="en-US">Biowallacea Press</dc:contributor>
	<dc:date>2016-10-11</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/wallacea/article/view/5851</dc:identifier>
	<dc:identifier>10.33772/biowallacea.v3i2.5851</dc:identifier>
	<dc:source xml:lang="en-US">BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research); Vol 3, No 2 (2016): Biodiversity in Wallacea</dc:source>
	<dc:source>2685-6360</dc:source>
	<dc:source>2355-6404</dc:source>
	<dc:source>10.33772/biowallacea.v3i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/wallacea/article/view/5851/4289</dc:relation>
	<dc:relation>https://ojs.uho.ac.id/index.php/wallacea/article/downloadSuppFile/5851/370</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2019 Jurnal BioWallacea</dc:rights>
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