Melola Discovery

← Melola Discovery

Jurnal

ISOLASI, KARAKTERISASI, DAN UJI POTENSI BAKTERI PENGHASIL ENZIM TERMOSTABIL DARI SUMBER AIR PANAS LAINEA, SULAWESI TENGGARA

BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) · Vol. 5 No. 1 (2018) · pp. 726–734

DOI
10.33772/biowallacea.v5i1.4592
ISSN
2685-6360, 2355-6404
Published
2018-04-28
Publisher
University of Halu Oleo
Language
eng

Abstract

ABSTRAK Penelitian ini bertujuan untuk memperoleh isolat bakteri yang berpotensi sebagai penghasil enzim ekstraseluler “Proliase” (Protease, Lipase, Amilase dan Selulase) dan mengetahui karakteristik isolat bakteri dari air panas Lainea. Tahapan-tahapan yang dilakukan dalam penelitian ini meliputi pengambilan sampel, isolasi bakteri, seleksi bakteri pada media spesifik serta karakterisasi bakteri potensial penghasil enzim ekstraseluler berdasarkan karakter fenotipik. Hasil penelitian ini diperoleh 36 isolat bakteri dan hanya terdapat 3 isolat bakteri yang memiliki potensi ganda dalam menghasilkan enzim ekstraseluler. Ketiga isolat bakteri memiliki bentuk sel batang, Gram positif, tidak menghasilkan endospora, bersifat motil, katalase positif, menunjukkan hasil negatif pada uji Indol, Methyl Red, Voges Proskauer, sitrat dan mampu memfermentasi sukrosa, glukosa dan mannitol, toleran terhadap konsentrasi NaCl 3-7%, pH 5-7 dan suhu 37-70oC. Ketiga isolat bakteri dari sumber air panas Lainea tersebut identik dengan genus acuan Listeria. Kata kunci: Air panas Lainea, Bakteri Proteolitik, Bakteri Lipolitik, Bakteri Amilolitik, Bakteri Selulolitik ABSTRACT This study aims to obtain bacterial isolates that have the potential as producers of "Proliase" extracellular enzymes (Protease, Lipase, Amylase and Cellulase) and determine the characteristics of bacterial isolates from Lainea hot water. The stages carried out in this study include sampling, bacterial isolation, bacterial selection in specific media and characterization of extracellular enzyme-producing potential bacteria based on phenotypic characters. The results of this study obtained 36 bacterial isolates and there were only 3 bacterial isolates which had double potential in producing extracellular enzymes. The three bacterial isolates have the form of stem cells, Gram positive, do not produce endospores, are motile, positive catalase, show negative results on the test of Indol, Methyl Red, Voges Proskauer, citrate and are able to ferment sucrose, glucose and mannitol, tolerant to NaCl 3- concentration 7%, pH 5-7 and temperature of 37-70oC. The three bacterial isolates from Lainea's hot springs are identical to Listeria's reference genus. Keywords: Lainea Hot Water, Proteolytic Bacteria, Lipolytic Bacteria, Amylolytic Bacteria, Cellulolytic Bacteria

Collaboration network of the authors

30 authors · 60 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Universitas Ahmad Dahlan
  • Universitas Bhayangkara Jakarta Raya
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Lili DarlianAffiliation unknown3
Mashuni MashuniAffiliation unknown2
Muhammad Aswan AswanAffiliation unknown1
Asrul SaniAffiliation unknown1
Sri AmbardiniUniversitas Halu Oleo1
Dirvamena BoerUniversitas Halu Oleo1
Darwis SulemanUniversitas Halu Oleo1
Susi IsrawatiAffiliation unknown1
M SirihAffiliation unknown1
Fahyuddin FahyuddinAffiliation unknown1
Santi SeptianaAffiliation unknown1
Sartina ButonAffiliation unknown1
Novi NurdiyantiAffiliation unknown1
Muh SyahruramadhanAffiliation unknown1
Andri PrionoAffiliation unknown1
Muzuni MuzuniAffiliation unknown1
Haidin HaidinAffiliation unknown1
Sitti Wirdhana AhmadAffiliation unknown1
Desty TryaswatyAffiliation unknown1
A. NurhanaAffiliation unknown1
Ardiansyah ArdiansyahUniversitas Ahmad Dahlan1
M. Rajab Sutra MijayaAffiliation unknown1
Nurhayani H. MuhiddinAffiliation unknown1
Syahrul HidayantoUniversitas Bhayangkara Jakarta Raya1
Ihsan RamadhanAffiliation unknown1
Ratna PuspitaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/wallacea/oai, first harvested 31 Okt 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">ISOLASI, KARAKTERISASI, DAN UJI POTENSI BAKTERI PENGHASIL ENZIM TERMOSTABIL DARI SUMBER AIR PANAS LAINEA, SULAWESI TENGGARA</dc:title>
	<dc:creator>Arji, Marwati</dc:creator>
	<dc:creator>Rezky, Wina</dc:creator>
	<dc:creator>Nurhaliza, Wa Ode Sitti</dc:creator>
	<dc:creator>Yanti, Nur Arfa</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">ABSTRAK Penelitian ini bertujuan untuk memperoleh isolat bakteri yang berpotensi sebagai penghasil enzim ekstraseluler “Proliase” (Protease, Lipase, Amilase dan Selulase) dan  mengetahui karakteristik isolat bakteri dari air panas Lainea. Tahapan-tahapan yang dilakukan dalam penelitian ini meliputi pengambilan sampel, isolasi bakteri, seleksi bakteri pada media spesifik serta karakterisasi bakteri potensial penghasil enzim ekstraseluler berdasarkan karakter fenotipik. Hasil penelitian ini diperoleh 36 isolat bakteri dan hanya terdapat 3 isolat bakteri yang memiliki potensi ganda dalam menghasilkan enzim ekstraseluler. Ketiga isolat bakteri memiliki bentuk sel batang, Gram positif, tidak menghasilkan endospora, bersifat motil, katalase positif, menunjukkan hasil negatif pada uji Indol, Methyl Red, Voges Proskauer, sitrat dan mampu memfermentasi sukrosa, glukosa dan mannitol, toleran terhadap konsentrasi NaCl 3-7%, pH 5-7 dan suhu 37-70oC. Ketiga isolat bakteri dari sumber air panas Lainea tersebut identik dengan genus acuan Listeria. Kata kunci : Air panas Lainea, Bakteri Proteolitik, Bakteri Lipolitik, Bakteri Amilolitik, Bakteri Selulolitik          ABSTRACT This study aims to obtain bacterial isolates that have the potential as producers of "Proliase" extracellular enzymes (Protease, Lipase, Amylase and Cellulase) and determine the characteristics of bacterial isolates from Lainea hot water. The stages carried out in this study include sampling, bacterial isolation, bacterial selection in specific media and characterization of extracellular enzyme-producing potential bacteria based on phenotypic characters. The results of this study obtained 36 bacterial isolates and there were only 3 bacterial isolates which had double potential in producing extracellular enzymes. The three bacterial isolates have the form of stem cells, Gram positive, do not produce endospores, are motile, positive catalase, show negative results on the test of Indol, Methyl Red, Voges Proskauer, citrate and are able to ferment sucrose, glucose and mannitol, tolerant to NaCl 3- concentration 7%, pH 5-7 and temperature of 37-70oC. The three bacterial isolates from Lainea's hot springs are identical to Listeria's reference genus. Keywords : Lainea Hot Water, Proteolytic Bacteria, Lipolytic Bacteria, Amylolytic Bacteria, Cellulolytic Bacteria</dc:description>
	<dc:publisher xml:lang="en-US">University of Halu Oleo</dc:publisher>
	<dc:contributor xml:lang="en-US">University of Halu Oleo Kendari</dc:contributor>
	<dc:date>2018-04-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/wallacea/article/view/4592</dc:identifier>
	<dc:identifier>10.33772/biowallacea.v5i1.4592</dc:identifier>
	<dc:source xml:lang="en-US">BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research); Vol 5, No 1 (2018): Sains &amp; Biodiversitas Wallacea; 726-734</dc:source>
	<dc:source>2685-6360</dc:source>
	<dc:source>2355-6404</dc:source>
	<dc:source>10.33772/biowallacea.v5i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/wallacea/article/view/4592/pdf</dc:relation>
	<dc:relation>https://ojs.uho.ac.id/index.php/wallacea/article/downloadSuppFile/4592/248</dc:relation>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:coverage xml:lang="en-US"></dc:coverage>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 Jurnal BioWallacea</dc:rights>
</oai_dc:dc>