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Uji Aktivitas Antioksidan Hidrolisat Protein Kerang Pasir (Semele cordiformis) dengan Metode DPPH (Antioxidant Activity of Protein Hydrolisate from Semele cordiformis Using DPPH Methode)

MEDULA (JURNAL ILMIAH FAKULTAS KEDOKTERAN UNIVERSITAS HALU OLEO) · Vol. 7 No. 2 (2020)

DOI
10.46496/medula.v7i2.11969
ISSN
2443-0218, 2339-1006
Published
2020-05-01
Publisher
Halu Oleo University
Language
eng

Abstract

Background: Sand shell (Semele cordiformis) is commonly found in South East Sulawesi marine area. This species traditionally used for treatment of several disease. Nevertheless, there is less publication about bioactivity of S. cordiformis as asource of drugs. Purpose: The aims of this research is to determine the activity of protein hydrolysate of S. cordiformis as an antioxidant. Methods: This research was conducted by pre-experimetal study with post test only control group design. Antioxidant activity was measure by DPPH method and spectrophotometry. The sample was hydrolyzed by using papain enzyme. The sample was made in to concentration 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm and vitamin C was used as positive control. The IC50 was used to measure antioxidant activity. Results: Concentration of 200 ppm showed 36,68% of inhibition percentage, 400 ppm showed 47,69%, 600 ppm showed 60,19%, 800 ppm showed 65,63%, and 1000 ppm showed 72,69 %. The IC50 value of was obtained for 453,777 ppm. Conclusion: The protein hydrolysate of S. cordiformis have an antioxidant activity and the hydrolisat concentration is directly proportional with inhibition activity.Keyword: antioxidant, protein hydrolysate, Semele cordiformis ABSTRAKPendahuluan: Kerang pasir atau Semele ccordiformis merupakan jenis kerang yang banyak ditemukan di perairan Sulawesi Tenggara. Kerang ini merupakah salah satu jenis kerang yang telah dimanfaatkan secara turun-temurun oleh masyarakat lokal untuk pengobatan. Meskipun demikian, belum ditemukan publikasi ilmiah mengenai bioaktifitas S. ccordiformis dalam bidang kesehatan. Tujuan: Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan hidrolisat protein S. cordiformis. Metode: Penelitian menggunakan desain pra-eksperimental dengan pendekatan post-test only control group. Uji aktivitas antioksidan menggunakan metode DPPH dengan prinsip spektrofotometri. Sampel dari penelitian ini adalah S. cordiformis yang diperoleh dari perairan Pulau Bokori, Kabupaten Konawe, Provinsi Sulawesi Tenggara yang kemudian dihidrolisis menggunakan enzim papain. Sampel dibuat menjadi beberapa konsentrasi yaitu 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm dan vitamin C digunakan sebagai kontrol positif. Nilai IC50 digunakan untuk mengetahui aktifitas antioksidan. Hasil: Konsentrasi 200 ppm menghasilkan persen inhibisi sebesar 36,68%, 400 ppm sebesar 47,69%, 600 ppm sebesar 60,19%, 800 ppm sebesar 65,63%, dan 1000 ppm sebesar 72,69 %. Nilai IC50 yang diperoleh adalah 453,777 ppm. Simpulan: hidrolisat protein S. cordiformis memiliki aktivitas antioksidan dengan konsentrasi hidrolisat berbanding lurus terhadap aktifitas inhibisi.Kata Kunci: antioksidan, hidrolisat protein, Semele cordiformis

Collaboration network of the authors

40 authors · 74 co-authorship links · 3 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Institut Agama Islam Negeri Kendari
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Wahyudin MaguniInstitut Agama Islam Negeri Kendari2
Beti MuluInstitut Agama Islam Negeri Kendari2
Juminten SaiminAffiliation unknown2
Satrio WicaksonoAffiliation unknown2
Lezdyana Nur IslamiAffiliation unknown2
Tien TienAffiliation unknown2
Fine Farhani MuliatiAffiliation unknown2
Fifit ErvithaAffiliation unknown2
Adzil Arsyi SabanaInstitut Agama Islam Negeri Kendari1
Eka Rizky SaputraInstitut Agama Islam Negeri Kendari1
Rahmi RahmiInstitut Agama Islam Negeri Kendari1
St NapisaInstitut Agama Islam Negeri Kendari1
Satriyani SatriyaniInstitut Agama Islam Negeri Kendari1
Irma ZulviantiInstitut Agama Islam Negeri Kendari1
Muhamad TurmudiInstitut Agama Islam Negeri Kendari1
Rahmi RahmiInstitut Agama Islam Negeri Kendari1
Asriyani Mulia BasriUniversitas Halu Oleo1
Murni NiaUniversitas Halu Oleo1
Muhamad ZamrudAffiliation unknown1
Sudarman SudarmanAffiliation unknown1
Saefuddin SaefuddinUniversitas Halu Oleo1
Muh. Ali MarhadiAffiliation unknown1
Sufiah Asri MulyawatiAffiliation unknown1
La RangkiAffiliation unknown1
Jamaluddin JamaluddinAffiliation unknown1
Ashaeryanto AshaeryantoAffiliation unknown1
Riszki RiszkiAffiliation unknown1
Imran - ImranAffiliation unknown1
Eka Suci FitriaAffiliation unknown1
Wa Ode Sitti Asfiah UduAffiliation unknown1
Andi Noor KholidhaAffiliation unknown1
Fifi NirmalaUniversitas Halu Oleo1
Wa Ode Sitti Asfiah UduAffiliation unknown1
Muhammad Handoyo SahumenaUniversitas Halu Oleo1
Nur Illiyyin AkibAffiliation unknown1
Yunita DawuAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/medula/oai, first harvested 31 Okt 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Uji Aktivitas Antioksidan Hidrolisat Protein Kerang Pasir (Semele cordiformis) dengan Metode DPPH  (Antioxidant Activity of Protein Hydrolisate from Semele cordiformis Using DPPH Methode)</dc:title>
	<dc:creator>Kalsum, Ummi</dc:creator>
	<dc:creator>Hafizah, Indria</dc:creator>
	<dc:creator>Aritrina, Pranita</dc:creator>
	<dc:creator>Sulastrianah, Sulastrianah</dc:creator>
	<dc:description xml:lang="en-US">Background: Sand shell (Semele cordiformis) is commonly found in South East Sulawesi marine area. This species traditionally used for treatment of several disease. Nevertheless, there is less publication about bioactivity of S. cordiformis as asource of drugs. Purpose: The aims of this research is to determine the activity of protein hydrolysate of S. cordiformis as an antioxidant. Methods: This research was conducted by pre-experimetal study with post test only control group design. Antioxidant activity was measure by DPPH method and spectrophotometry. The sample was hydrolyzed by using papain enzyme. The sample was made in to concentration 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm and vitamin C was used as positive control. The IC50 was used to measure antioxidant activity. Results: Concentration of 200 ppm showed 36,68% of inhibition percentage, 400 ppm showed 47,69%, 600 ppm showed 60,19%, 800 ppm showed 65,63%, and 1000 ppm showed 72,69 %. The IC50 value of was obtained for 453,777 ppm. Conclusion: The protein hydrolysate of S. cordiformis have an antioxidant activity and the hydrolisat concentration is directly proportional with inhibition activity.Keyword: antioxidant, protein hydrolysate, Semele cordiformis ABSTRAKPendahuluan: Kerang pasir atau Semele ccordiformis merupakan jenis kerang yang banyak ditemukan di perairan Sulawesi Tenggara. Kerang ini merupakah salah satu jenis kerang yang telah dimanfaatkan secara turun-temurun oleh masyarakat lokal untuk pengobatan. Meskipun demikian, belum ditemukan publikasi ilmiah mengenai bioaktifitas S. ccordiformis dalam bidang kesehatan. Tujuan: Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan hidrolisat protein S. cordiformis. Metode: Penelitian menggunakan desain pra-eksperimental dengan pendekatan post-test only control group. Uji aktivitas antioksidan menggunakan metode DPPH dengan prinsip spektrofotometri. Sampel dari penelitian ini adalah S. cordiformis yang diperoleh dari perairan Pulau Bokori, Kabupaten Konawe, Provinsi Sulawesi Tenggara yang kemudian dihidrolisis menggunakan enzim papain. Sampel dibuat menjadi beberapa konsentrasi yaitu 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1000 ppm dan vitamin C digunakan sebagai kontrol positif. Nilai IC50 digunakan untuk mengetahui aktifitas antioksidan. Hasil: Konsentrasi 200 ppm menghasilkan persen inhibisi sebesar 36,68%, 400 ppm sebesar 47,69%, 600 ppm sebesar 60,19%, 800 ppm sebesar 65,63%, dan 1000 ppm sebesar 72,69 %. Nilai IC50 yang diperoleh adalah 453,777 ppm. Simpulan: hidrolisat protein S. cordiformis memiliki aktivitas antioksidan dengan konsentrasi hidrolisat berbanding lurus terhadap aktifitas inhibisi.Kata Kunci: antioksidan, hidrolisat protein, Semele cordiformis</dc:description>
	<dc:publisher xml:lang="en-US">Halu Oleo University</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2020-05-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/medula/article/view/11969</dc:identifier>
	<dc:identifier>10.46496/medula.v7i2.11969</dc:identifier>
	<dc:source xml:lang="en-US">MEDULA (JURNAL ILMIAH FAKULTAS KEDOKTERAN UNIVERSITAS HALU OLEO); Vol 7, No 2 (2020)</dc:source>
	<dc:source>2443-0218</dc:source>
	<dc:source>2339-1006</dc:source>
	<dc:source>10.46496/medula.v7i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/medula/article/view/11969/8424</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 MEDULA</dc:rights>
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