Melola Discovery

← Melola Discovery

Jurnal

Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro

JURNAL BIOLOGI PAPUA · Vol. 12 No. 1 (2020) · pp. 43–49

DOI
10.31957/jbp.1096
ISSN
2503-0450, 2086-3314
Published
2020-03-05
Publisher
Jurusan Biologi FMIPA Universitas Cenderawasih
Language
eng

Abstract

The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0%, 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet in vitro.Keywords: Chitosan, orchid, Vacin and Went, plantlet.

Collaboration network of the authors

40 authors · 159 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Suharno SuharnoAffiliation unknown14
Supeni SufaatiAffiliation unknown11
Puguh SujartaAffiliation unknown6
Farmawaty FarmawatyAffiliation unknown6
Roma M. ManaluAffiliation unknown3
Eva S. SimaremareAffiliation unknown2
Suriani Br SurbaktiAffiliation unknown2
Ign Joko SuyonoAffiliation unknown2
Agnes Eri MaryuniAffiliation unknown2
Johnson SiallaganAffiliation unknown2
Tri GunaediAffiliation unknown2
I Made BudiAffiliation unknown2
Suharno -Affiliation unknown2
Wilda Z. MuhhidaAffiliation unknown2
Vita PurnamasariAffiliation unknown2
Marseline G. MailisaAffiliation unknown2
Irene SuebuAffiliation unknown2
Wehelmina LahalloAffiliation unknown2
Nani YuniarUniversitas Halu Oleo1
Andi Faizal FachlevyAffiliation unknown1
Sri WilujengAffiliation unknown1
Rosye H.R. TanjungAffiliation unknown1
Suharyanto SuharyantoAffiliation unknown1
Lisiard DimaraAffiliation unknown1
Chris D. SembayAffiliation unknown1
Elsye GunawanAffiliation unknown1
Jane AwomAffiliation unknown1
Susan WopiAffiliation unknown1
Henderina J. KeiluhuAffiliation unknown1
Agnes E. MaryuniAffiliation unknown1
Daniel Z. K. WambrauwAffiliation unknown1
Cheny AdetyaningsihAffiliation unknown1
Lisye I. ZebuaAffiliation unknown1
Meinarni AsnawiAffiliation unknown1
TRIWIYONO TRIWIYONOAffiliation unknown1
Anastasia NumberiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ejournal.uncen.ac.id/index.php/JBP/oai, first harvested 1 Nov 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro</dc:title>
	<dc:creator>Agustini, Verena</dc:creator>
	<dc:creator>Rahayu, Irma</dc:creator>
	<dc:creator>Numberi, Leonardo A.</dc:creator>
	<dc:creator>Ni’mah, Ziyadatun</dc:creator>
	<dc:description xml:lang="en-US">The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm  and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet  in vitro.Keywords : Chitosan, orchid, Vacin and Went, plantlet.</dc:description>
	<dc:publisher xml:lang="en-US">Jurusan Biologi FMIPA Universitas Cenderawasih</dc:publisher>
	<dc:date>2020-03-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1096</dc:identifier>
	<dc:identifier>10.31957/jbp.1096</dc:identifier>
	<dc:source xml:lang="en-US">JURNAL BIOLOGI PAPUA; Vol. 12 No. 1 (2020); 43-49</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biologi Papua; Vol 12 No 1 (2020); 43-49</dc:source>
	<dc:source>2503-0450</dc:source>
	<dc:source>2086-3314</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1096/957</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 JURNAL BIOLOGI PAPUA</dc:rights>
</oai_dc:dc>