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Uji Aktivitas Sitotoksik Fraksi Daun Matoa (Pometia pinnata) dengan Metode Brine Shrimp Lethality Test (BSLT)

JURNAL BIOLOGI PAPUA · Vol. 13 No. 2 (2021) · pp. 83–91

DOI
10.31957/jbp.1358
ISSN
2503-0450, 2086-3314
Published
2021-10-01
Publisher
Jurusan Biologi FMIPA Universitas Cenderawasih
Language
eng

Abstract

Matoa (Pometia pinnata) is parts of Sapindaceae family widely spread in tropical areas, especially in Papua. Several studies mentioned the use of its parts as antioxidants and contain phenolic compounds and flavonoids. This study aim(s) to conduct a phytochemical screening test as well as a cytotoxic test for Pometia pinnata leaves fraction using the BSLT method. The research series began with the extraction process using 96% ethanol, then fractionated using three solvent variations: n-hexane, ethyl acetate, and ethanol. Further, the phytochemical screening test was carried out for cytotoxic testing in order to see the mortality rate of shrimp larvae Artemia salina Leach. The result of the phytochemical showed that the secondary metabolite compounds on the ethanol and ethyl acetate fraction were alkaloids, flavonoids, saponins, tannins, and terpenoids compounds meanwhile the n-hexane fraction contained terpenoids. The results of cytotoxic testing BSLT showed the LC50 value in the n-hexane was 7029.10 ppm, 406.07 ppm for ethyl acetate and 614.47 ppm for ethanol. Thus, it could be said that ethyl acetate and ethanol fractions were classified as a toxic category, while n-hexane was classified as a non-toxic category.Key words: matoa; fraction; phytochemical screening; cytotoxic.

Collaboration network of the authors

37 authors · 159 co-authorship links

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Co-authorOrganisationJoint articles
Eva S. SimaremareAffiliation unknown4
Rusnaeni RusnaeniAffiliation unknown3
Maria T. NainggolanAffiliation unknown2
Eva Susanty SimaremareAffiliation unknown2
Septriyanto DirgantaraAffiliation unknown2
Rani Dewi PratiwiAffiliation unknown2
Deshinta S. NataliaAffiliation unknown2
Noviana HallikAffiliation unknown2
Nur F. BakriAffiliation unknown2
Verena AgustiniAffiliation unknown1
Jane AwomAffiliation unknown1
Susan WopiAffiliation unknown1
Ega RianaAffiliation unknown1
Nur F. BakrieAffiliation unknown1
Aris A.L. ToruanAffiliation unknown1
Felycitae E. AppaAffiliation unknown1
Maria J.S OlaAffiliation unknown1
RusnaeniAffiliation unknown1
Sarce M. SasarariAffiliation unknown1
Allowisya FutwembunAffiliation unknown1
Khoirul HudaAffiliation unknown1
Qonita A. NisaAffiliation unknown1
Yuliana R. YabansabraAffiliation unknown1
Tatang SutarmanAffiliation unknown1
Andre M. WorabaiAffiliation unknown1
Dimas A. SyafrudinAffiliation unknown1
Fadil M. Al RidhaAffiliation unknown1
Karlin MansumberAffiliation unknown1
Kristina I. RahailAffiliation unknown1
Marselina E. KotenAffiliation unknown1
Muhammad M. KhalilAffiliation unknown1
Ota M. RumainumAffiliation unknown1
Pitera WendaAffiliation unknown1
Sarhana R.A. SaidAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ejournal.uncen.ac.id/index.php/JBP/oai, first harvested 1 Nov 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Uji Aktivitas Sitotoksik Fraksi Daun Matoa (Pometia pinnata) dengan Metode Brine Shrimp Lethality Test (BSLT)</dc:title>
	<dc:creator>Payung, Risma</dc:creator>
	<dc:creator>Gunawan, Elsye</dc:creator>
	<dc:creator>Pratiwi, Rani D.</dc:creator>
	<dc:description xml:lang="en-US">Matoa (Pometia pinnata) is parts of Sapindaceae family widely spread in tropical areas, especially in Papua. Several studies mentioned the use of its parts as antioxidants and contain phenolic compounds and flavonoids. This study aim(s) to conduct a phytochemical screening test as well as a cytotoxic test for Pometia pinnata leaves fraction using the BSLT method. The research series began with the extraction process using 96% ethanol, then fractionated using three solvent variations: n-hexane, ethyl acetate, and ethanol. Further, the phytochemical screening test was carried out for cytotoxic testing in order to see the mortality rate of shrimp larvae Artemia salina Leach. The result of the phytochemical showed that the secondary metabolite compounds on the ethanol and ethyl acetate fraction were alkaloids, flavonoids, saponins, tannins, and terpenoids compounds meanwhile the n-hexane fraction contained terpenoids. The results of cytotoxic testing BSLT showed the LC50 value in the n-hexane was 7029.10 ppm, 406.07 ppm for ethyl acetate and 614.47 ppm for ethanol. Thus, it could be said that ethyl acetate and ethanol fractions were classified as a toxic category, while n-hexane was classified as a non-toxic category.Key words: matoa; fraction; phytochemical screening; cytotoxic.</dc:description>
	<dc:publisher xml:lang="en-US">Jurusan Biologi FMIPA Universitas Cenderawasih</dc:publisher>
	<dc:date>2021-10-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1358</dc:identifier>
	<dc:identifier>10.31957/jbp.1358</dc:identifier>
	<dc:source xml:lang="en-US">JURNAL BIOLOGI PAPUA; Vol. 13 No. 2 (2021); 83 - 91</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biologi Papua; Vol 13 No 2 (2021); 83 - 91</dc:source>
	<dc:source>2503-0450</dc:source>
	<dc:source>2086-3314</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1358/1378</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 JURNAL BIOLOGI PAPUA</dc:rights>
</oai_dc:dc>