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Quantitative Analysis of Flavonoid Content, Total Phenolic and Toxicity Test of Extract Smilax rotundifolia Leaves From Papua

JURNAL BIOLOGI PAPUA · Vol. 17 No. 1 (2025) · pp. 29–36

DOI
10.31957/jbp.4539
ISSN
2503-0450, 2086-3314
Published
2025-06-14
Publisher
Jurusan Biologi FMIPA Universitas Cenderawasih
Language
eng

Abstract

This study investigates Bungkus leaf (Smilax rotundifolia), a well-known plant in Papua commonly referred to as the three-finger leaf. Indigenous Papuans traditionally use this plant to enlarge genitals, buttocks, and breasts, as well as to treat syphilis. The plant is known to contain flavonoid and phenolic compounds with pharmacological activity. This study aimed to determine the total flavonoid and total phenolic content of the 70% ethanol extract of Bungkus leaf. The research began with simplicia preparation, followed by extraction using 70% ethanol. Qualitative tests, including phytochemical screening and thin-layer chromatography (TLC), were conducted, followed by quantitative analysis using UV-Vis spectrophotometry. The results showed total flavonoid content of 54.696 mg QE/g ± 0.565 and total phenolic content of 32.908 mg GAE/g ± 0.263. The study concluded that the total flavonoid content was higher than the total phenolic content. Toxicity testing categorized the extract as toxic, with an LC50 value of 442.92 ppm.

Keywords

Collaboration network of the authors

16 authors · 43 co-authorship links

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Co-authorOrganisationJoint articles
Artha M. SimanjuntakAffiliation unknown3
Agatha C.I. JedautAffiliation unknown3
Napita P.C. PasaribuAffiliation unknown3
Elsye GunawanAffiliation unknown2
Rusnaeni RusnaeniAffiliation unknown1
Aris A.L. ToruanAffiliation unknown1
Rani D. PratiwiAffiliation unknown1
Maria J.S OlaAffiliation unknown1
RusnaeniAffiliation unknown1
Sarce M. SasarariAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ejournal.uncen.ac.id/index.php/JBP/oai, first harvested 1 Nov 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Quantitative Analysis of Flavonoid Content, Total Phenolic and Toxicity Test of Extract Smilax rotundifolia Leaves From Papua</dc:title>
	<dc:creator>Appa, Felycitae E.</dc:creator>
	<dc:creator>Tobi, Claudius H.B.</dc:creator>
	<dc:creator>Suprapti, Kevry</dc:creator>
	<dc:creator>Bakri, Nur F.</dc:creator>
	<dc:creator>Barus, Andre A.</dc:creator>
	<dc:creator>Pratiwi, Mustika E.</dc:creator>
	<dc:subject xml:lang="en-US">BSLT</dc:subject>
	<dc:subject xml:lang="en-US">Smilax rotundifolia </dc:subject>
	<dc:subject xml:lang="en-US">spectrophotometry </dc:subject>
	<dc:subject xml:lang="en-US">total flavonoids </dc:subject>
	<dc:subject xml:lang="en-US">total phenolic</dc:subject>
	<dc:description xml:lang="en-US">This study investigates Bungkus leaf (Smilax rotundifolia), a well-known plant in Papua commonly referred to as the three-finger leaf. Indigenous Papuans traditionally use this plant to enlarge genitals, buttocks, and breasts, as well as to treat syphilis. The plant is known to contain flavonoid and phenolic compounds with pharmacological activity. This study aimed to determine the total flavonoid and total phenolic content of the 70% ethanol extract of Bungkus leaf. The research began with simplicia preparation, followed by extraction using 70% ethanol. Qualitative tests, including phytochemical screening and thin-layer chromatography (TLC), were conducted, followed by quantitative analysis using UV-Vis spectrophotometry. The results showed total flavonoid content of 54.696 mg QE/g ± 0.565 and total phenolic content of 32.908 mg GAE/g ± 0.263. The study concluded that the total flavonoid content was higher than the total phenolic content. Toxicity testing categorized the extract as toxic, with an LC50 value of 442.92 ppm. </dc:description>
	<dc:publisher xml:lang="en-US">Jurusan Biologi FMIPA Universitas Cenderawasih</dc:publisher>
	<dc:date>2025-06-14</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal.uncen.ac.id/index.php/JBP/article/view/4539</dc:identifier>
	<dc:identifier>10.31957/jbp.4539</dc:identifier>
	<dc:source xml:lang="en-US">JURNAL BIOLOGI PAPUA; Vol. 17 No. 1 (2025); 29-36</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biologi Papua; Vol 17 No 1 (2025); 29-36</dc:source>
	<dc:source>2503-0450</dc:source>
	<dc:source>2086-3314</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.uncen.ac.id/index.php/JBP/article/view/4539/3202</dc:relation>
</oai_dc:dc>