Melola Discovery

← Melola Discovery

Jurnal

Isolation and Potency of Symbiotic Nitrogen Fixation Bacteria from Nodules of Falcataria moluccana (Miq.) Barneby & J.W.Grimes for Supporting Nickle Postmining Area Reclamation

Jurnal Penelitian Kehutanan Wallacea · Vol. 9 No. 2 (2020) · pp. 111–120

DOI
10.18330/jwallacea.2020.vol9iss2pp111-120
ISSN
2407-7860, 2302-299X
Published
2020-08-31
Publisher
Foresty Faculty of Hasanuddin University
Language
en

Abstract

Nickel mine is one of the contributors foreign to exchange earners in Indonesia. However, the former mining area creates a complex problem in the physical, chemical, and biological soil properties which directly affect the ability of plant growth for restoration. The planting of legume species, one of them is sengon laut (Falcataria moluccana), in the reclamation of ex-mining land aims to improve soil properties. Legume plants have low C/N ratio so the introduction of nitrogen-fixing soil microorganisms is one alternative that can be used to improvesoil fertility. Nitrogen fixing bacteria can provide nitrogen compounds that can be directly utilized by plants. In addition, the utilization of fast-growing species such as sengon laut is suitable for rehabilitation of critical lands. This study aims to isolate and characterize symbiotic nitrogen fixation bacteria so it can be made as inoculum and applied on revegetation in nickel postmining area. Five bacterial isolates were obtained from nodules of sengon laut belonging to genus Rhizobium(3 isolates) and Bradyrhizobium(2 isolates). All isolates obtained were rod-shaped, Gram negative and did not have endospores. Physiological tests showed that all isolates were aerobic, motile, grew optimally on YEMA media at pH 6 and 7, but did not grow at pH 4. Initial potential testof Acetylen Reduction Assay and IAA test showed that A3.1 SL 5 isolate has the highest value (9.01 ppm and 0.447 ppm) and potential to be inoculum.

Keywords

Collaboration network of the authors

3 authors · 2 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
NursyamsiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/wallacea/oai, first harvested 2 Nov 2026, last seen 2 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Isolation and Potency of Symbiotic Nitrogen Fixation Bacteria from Nodules of Falcataria moluccana (Miq.) Barneby &amp; J.W.Grimes for Supporting Nickle Postmining Area Reclamation</dc:title>
	<dc:creator xml:lang="en">Sari, Ramdana </dc:creator>
	<dc:creator xml:lang="en">Prayudyaningsih, Retno </dc:creator>
	<dc:subject xml:lang="en">Isolation</dc:subject>
	<dc:subject xml:lang="en">Sengon</dc:subject>
	<dc:subject xml:lang="en">Nitrogen</dc:subject>
	<dc:subject xml:lang="en">Fixation</dc:subject>
	<dc:subject xml:lang="en">Rhizobium</dc:subject>
	<dc:subject xml:lang="en">Bradyrhizobium</dc:subject>
	<dc:description xml:lang="en">Nickel mine is one of the contributors foreign to exchange earners in Indonesia. However, the former mining area creates a complex problem in the physical, chemical, and biological soil properties which directly affect the ability of plant growth for restoration. The planting of legume species, one of them is sengon laut (Falcataria moluccana), in the reclamation of ex-mining land aims to improve soil properties. Legume plants have low C/N ratio so the introduction of nitrogen-fixing soil microorganisms is one alternative that can be used to improvesoil fertility. Nitrogen fixing bacteria can provide nitrogen compounds that can be directly utilized by plants. In addition, the utilization of fast-growing species such as sengon laut is suitable for rehabilitation of critical lands. This study aims to isolate and characterize symbiotic nitrogen fixation bacteria so it can be made as inoculum and applied on revegetation in nickel postmining area. Five bacterial isolates were obtained from nodules of sengon laut belonging to genus Rhizobium(3 isolates) and Bradyrhizobium(2 isolates). All isolates obtained were rod-shaped, Gram negative and did not have endospores. Physiological tests showed that all isolates were aerobic, motile, grew optimally on YEMA media at pH 6 and 7, but did not grow at pH 4. Initial potential testof Acetylen Reduction Assay and IAA test showed that A3.1 SL 5 isolate has the highest value (9.01 ppm and 0.447 ppm) and potential to be inoculum.</dc:description>
	<dc:publisher xml:lang="en">Foresty Faculty of Hasanuddin University</dc:publisher>
	<dc:date>2020-08-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/wallacea/article/view/26209</dc:identifier>
	<dc:identifier>10.18330/jwallacea.2020.vol9iss2pp111-120</dc:identifier>
	<dc:source xml:lang="en">Jurnal Penelitian Kehutanan Wallacea; Vol. 9 No. 2 (2020); 111-120</dc:source>
	<dc:source>2407-7860</dc:source>
	<dc:source>2302-299X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/wallacea/article/view/26209/9558</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Jurnal Penelitian Kehutanan Wallacea</dc:rights>
</oai_dc:dc>