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Characteristics of Bali Bull (Bos Sondaicus) Sexed Sperms with Freeze Dry Egg White at Different Incubation Time: english

Hasanuddin Journal of Animal Science (HAJAS) · Vol. 4 No. 1 (2022) · hlm. 30–40

DOI
10.20956/hajas.v4i1.19259
ISSN
2621-9190, 2621-9182
Terbit
2022-06-06
Penerbit
Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
Bahasa
en
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

The development of reproductive technology for the separation of spermatozoa with X and Y chromosomes, which is better known as sexing, can be carried out in vitro using various methods, one of which is using freeze dry egg whites. This study aims to determine the quality of semen based on incubation time. This study used four types of treatment, namely P0 = No Incubation, P1 = Incubation 30 minutes, P2 = Incubation 45 minutes, P3 = Incubation 60 minutes with six replications. Parameters observed were the quality of spermatozoa macroscopically (volume, odor, color and consistency) and microscopically (motility, abnormality, concentration, intact plasma membrane, intact acrosome cap). Y using descriptive statistical test. The results of analysis of variance that sexing spermatozoa using freeze dry egg whites at different incubation times showed a significant effect (P 0.05) on concentration, motility, and abnormalities. Evaluation of fresh Bali cattle spermatozoa showed results that were in accordance with the standards for further examination of semen sexing. Incubation time of 30 minutes in the sexing process of spermatozoa caused a significant decrease in motility and concentration, meanwhile, IPM and IAM of sexed spermatozoa experienced a significant decrease in incubation time of 60 minutes, but the decrease was still within the normal range for spermatozoa. to be used in the artificial insemination process. Keywords: Sexing, incubation, concentration, motility, abnormality, intact plasma membrane, intact acrosome caps.

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  • Hasanuddin University
  • Universitas Ahmad Dahlan
  • Universitas Halu Oleo
  • Politeknik Pertanian Negeri Pangkajene Kepulauan
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Tampilkan sebagai tabel
Rekan penulisOrganisasiArtikel bersama
Zulkharnaim MaupaAfiliasi tidak diketahui2
Lellah RahimHasanuddin University2
Sudirman BacoHasanuddin University2
Abdul Latief TolengHasanuddin University2
Muhammad YusufUniversitas Halu Oleo2
Masturi MasturiHasanuddin University2
Toleng Abd LatiefAfiliasi tidak diketahui2
Nur SalmahHasanuddin University2
Hasrin HasrinHasanuddin University2
Ummul MasirPoliteknik Pertanian Negeri Pangkajene Kepulauan2
Imam RiadiUniversitas Ahmad Dahlan1
Sunardi SunardiUniversitas Ahmad Dahlan1
la ode Maksar MuhurunaAfiliasi tidak diketahui1
Djoni Prawira RahardjaAfiliasi tidak diketahui1
Abdul Latif TolengAfiliasi tidak diketahui1
Rika HaryaniAfiliasi tidak diketahui1
mawardi A. Asja ZulkarnaimAfiliasi tidak diketahui1
Muhammad Ihsan Andi DagongAfiliasi tidak diketahui1
Lellah RahimAfiliasi tidak diketahui1
Isdam SupriadiAfiliasi tidak diketahui1
Renny Fatmyah UtamyHasanuddin University1
Nur Eni NurHasanuddin University1
Dina ArdianaHasanuddin University1
Zulkharnaim ZulkharnaimHasanuddin University1
E. DondatuHasanuddin University1
Jasmal Ahmari SyamsuAfiliasi tidak diketahui1
Rinduwati RinduwatiAfiliasi tidak diketahui1
Sema SemaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/hajas/oai, pertama di-harvest 2 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
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	<dc:title xml:lang="en">Characteristics of Bali Bull (Bos Sondaicus) Sexed Sperms with Freeze Dry Egg White at Different Incubation Time: english</dc:title>
	<dc:creator xml:lang="en">Damayanti, Putri</dc:creator>
	<dc:creator xml:lang="en">Yusuf, Muhammad</dc:creator>
	<dc:creator xml:lang="en">Ako, Ambo</dc:creator>
	<dc:creator xml:lang="en">Sahiruddin, Sahiruddin</dc:creator>
	<dc:description xml:lang="en">The development of reproductive technology for the separation of spermatozoa with X and Y chromosomes, which is better known as sexing, can be carried out in vitro using various methods, one of which is using freeze dry egg whites. This study aims to determine the quality of semen based on incubation time. This study used four types of treatment, namely P0 = No Incubation, P1 = Incubation 30 minutes, P2 = Incubation 45 minutes, P3 = Incubation 60 minutes with six replications. Parameters observed were the quality of spermatozoa macroscopically (volume, odor, color and consistency) and microscopically (motility, abnormality, concentration, intact plasma membrane, intact acrosome cap). Y using descriptive statistical test. The results of analysis of variance that sexing spermatozoa using freeze dry egg whites at different incubation times showed a significant effect (P&amp;lt;0.05) on intact plasma membrane (IPM), intact acrosomal membrane (IAM), but had no significant effect P&amp;gt;0.05) on concentration, motility, and abnormalities. Evaluation of fresh Bali cattle spermatozoa showed results that were in accordance with the standards for further examination of semen sexing. Incubation time of 30 minutes in the sexing process of spermatozoa caused a significant decrease in motility and concentration, meanwhile, IPM and IAM of sexed spermatozoa experienced a significant decrease in incubation time of 60 minutes, but the decrease was still within the normal range for spermatozoa. to be used in the artificial insemination process.
Keywords:  Sexing, incubation, concentration, motility, abnormality, intact plasma membrane, intact acrosome caps.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia</dc:publisher>
	<dc:date>2022-06-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/hajas/article/view/19259</dc:identifier>
	<dc:identifier>10.20956/hajas.v4i1.19259</dc:identifier>
	<dc:source xml:lang="en">Hasanuddin Journal of Animal Science (HAJAS); Vol. 4 No. 1 (2022); 30-40</dc:source>
	<dc:source>2621-9190</dc:source>
	<dc:source>2621-9182</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/hajas/article/view/19259/8233</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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