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Antibacterial Activity Test of Combination Extract of Moringa oleifera Leaf and Red Ginger Against Escherichia coli and Staphylococcus aureus as Natural Feed Additive

Hasanuddin Journal of Animal Science (HAJAS) · Vol. 4 No. 1 (2022) · pp. 58–67

DOI
10.20956/hajas.v4i1.20042
ISSN
2621-9190, 2621-9182
Published
2022-10-01
Publisher
Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
Language
en
License
creativecommons.org/licenses/by-nc/4.0

Abstract

ABSTRACT Natural feed additives used such as moringa leaves, red ginger as antibacterial are the solution in preventing disease and can improve the performance of poultry. The purpose of the study was to determine the ability of the inhibition zone of the combination extract of Moringa leaves and red ginger against Escherichia coli and Staphylococcus aureus bacteria. The design used was RAL (Completely Randomized Design), Duncan's test and T test to determine the difference between each replication consisting of 5 treatments and 4 replications with treatment P0 (positive control, namely contrimexasol), P1 (Kelor leaves 40% + Red Ginger 30 %), P2 (Moringa Leaf 40% + Red Ginger 35%), P3 (Moringa Leaf 40% + Red Ginger 40%), P4 (Moringa Leaf 40% + Red Ginger 45%). The results showed that the inhibition zone of the combination extract of Moringa leaves and red ginger had a significant effect (P<0.05) on Escherichia coli and Staphylococcus aureus bacteria. The highest inhibition zones were in treatment P4 (Kelor leaves 40% + Red Ginger 45%) Escherichia coli bacteria 23.50 mm ± 0.11 mm and Staphylococus aureus 23.60 mm ± 0.11 mm. The conclusion is that the combination extract of Moringa leaves and red ginger with a concentration (40% Moringa leaves + 45% red ginger) was able to inhibit the growth of gram-positive and gram-negative bacteria with very strong inhibitory power. Keywords: Moringa leaf, Escherichia coli, feed additive, red ginger, Staphylococcus aureus

Collaboration network of the authors

12 authors · 17 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Universitas Islam Negeri Maulana Malik Ibrahim
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Takdir SailiUniversitas Halu Oleo1
La Ode NafiuUniversitas Halu Oleo1
Tambunan TambunanAffiliation unknown1
La MilihaAffiliation unknown1
Nani Restati SiregarAffiliation unknown1
Ratmawati MalakaAffiliation unknown1
Jamilah JamilahUniversitas Islam Negeri Maulana Malik Ibrahim1
Erika RahayuAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/hajas/oai, first harvested 2 Nov 2026, last seen 2 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Antibacterial Activity Test of Combination Extract of Moringa oleifera Leaf and Red Ginger  Against Escherichia coli and Staphylococcus aureus as Natural Feed Additive </dc:title>
	<dc:creator xml:lang="en">Sunarti, Sunarti</dc:creator>
	<dc:creator xml:lang="en">Lahay, Nancy </dc:creator>
	<dc:creator xml:lang="en">Latief, Muhammad Fadhlirrahman </dc:creator>
	<dc:creator xml:lang="en">Gondipon, Reynard</dc:creator>
	<dc:description xml:lang="en">ABSTRACT
Natural &amp;nbsp;feed additives used such as moringa leaves, red ginger as antibacterial are the solution in preventing disease and can improve the performance of poultry . The purpose of the study was to determine the ability of the inhibition zone of the combination extract of Moringa leaves and red ginger against Escherichia coli and Staphylococcus aureus bacteria . The design used was RAL (Completely Randomized Design), Duncan's test and T test to determine the difference between each replication consisting of 5 treatments and 4 replications with treatment P0 (positive control, namely contrimexasol), P1 (Kelor leaves 40% + Red Ginger 30 %), P2 (Moringa Leaf 40% + Red Ginger 35%), P3 (Moringa Leaf 40% + Red Ginger 40%), P4 (Moringa Leaf 40% + Red Ginger 45%). The results showed that the inhibition zone of the combination extract of Moringa leaves and red ginger had a significant effect (P&amp;lt;0.05) on Escherichia coli and Staphylococcus aureus bacteria . The highest inhibition zones were in treatment P4 (Kelor leaves 40% + Red Ginger 45%) Escherichia coli bacteria 23.50 mm ± 0.11 mm and Staphylococus aureus 23.60 mm ± 0.11 mm. The conclusion is that the combination extract of Moringa leaves and red ginger with a concentration (40% Moringa leaves + 45% red ginger) was able to inhibit the growth of gram-positive and gram-negative bacteria with very strong inhibitory power.
Keywords: Moringa leaf, Escherichia coli, feed additive, red ginger , Staphylococcus aureus
&amp;nbsp;</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia</dc:publisher>
	<dc:date>2022-10-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/hajas/article/view/20042</dc:identifier>
	<dc:identifier>10.20956/hajas.v4i1.20042</dc:identifier>
	<dc:source xml:lang="en">Hasanuddin Journal of Animal Science (HAJAS); Vol. 4 No. 1 (2022); 58-67</dc:source>
	<dc:source>2621-9190</dc:source>
	<dc:source>2621-9182</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/hajas/article/view/20042/8726</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>