Ethanolic extract of black grapes (Vitis Vinifera) ameliorates overtraining-induced pancreatic β-cells and muscle damage

Siswanto, Ferbian Milas; Pradhany, Risha Catra; Yenniastoeti, Boedi Prihatini; Pangkahila, Alex

Open access Jurnal

Ethanolic extract of black grapes (Vitis Vinifera) ameliorates overtraining-induced pancreatic β-cells and muscle damage

Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) · Vol. UME No. 2 (2020)

ISSN: 2615-2835, 2614-0187
Published: 2020-11-05
Publisher: Hasanuddin University
Language: en
License: creativecommons.org/licenses/by-nc/4.0

Abstract

Overtraining is a high-volume, high-intensity, long-duration, and high-frequency training. The heavier physical training, the more oxygen needed for metabolism. Increasing oxygen usage causes an elevation of electron leakage in mitochondria;thus,produce a higher amount of reactive oxygen species (ROS). Antioxidant inhibits oxidative damage in a target molecule. Grapes contain a lot of antioxidants, such as polyphenols and anthocyanins. The purpose of this study was to examine the effect of ethanol extract of Balinese grapes (Vitis vinifera) on β-cells and muscle damage in overtraining-induced rats. This study was a completely randomized experimental study using a posttest only control group design. Samples were 36 male albino rats (Rattus norvergicus), aged 2.5-3 months, divided randomly into two groups. The control group (P0), 18 rats, were given overtraining and placebo of 2 ml distilled water; the treatment group (P1) 18 rats, were given overtraining and 25g/kg.BW ethanol extract of Balinese grapes. The result showed the average amount of pancreatic β-cells in the P0 group was higher than the P1 group (p< 0.001). In addition, the average level of creatine kinase was also higher in the P1 group than those of the P0 group (p<0,001). It can be concluded that the administration of ethanolic extract of Balinese grapes mitigates the damage on pancreatic β-cells and muscle cells induced with overtraining.

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Co-author	Organisation	Joint articles
Dian Fatmawati	Affiliation unknown	1
Musdalifah Musdalifah	Affiliation unknown	1
Yuliani Suparmin	Affiliation unknown	1
Nur Afzah Zainuddin	Affiliation unknown	1
Eka Nurdiana	Affiliation unknown	1
William William	Affiliation unknown	1
Maria Dara Novi Handayani	Affiliation unknown	1
Hotmaria Agustina Pakpahan	Affiliation unknown	1
Rita Dewi	Affiliation unknown	1
Ian Pranandi	Affiliation unknown	1
Evi Ulina M Situmorang	Affiliation unknown	1
Jojor Lamsihar Manalu	Affiliation unknown	1
Ana Lucia Ekowati	Affiliation unknown	1
Yulia Tanti Narwati	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/jrvi/oai, first harvested 3 Nov 2026, last seen 3 Nov 2026.

Original metadata (oai_dc)

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	<dc:title xml:lang="en">Ethanolic extract of black grapes (Vitis Vinifera) ameliorates overtraining-induced pancreatic β-cells and muscle damage</dc:title>
	<dc:creator xml:lang="en">Siswanto, Ferbian Milas</dc:creator>
	<dc:creator xml:lang="en">Pradhany, Risha Catra</dc:creator>
	<dc:creator xml:lang="en">Yenniastoeti, Boedi Prihatini</dc:creator>
	<dc:creator xml:lang="en">Pangkahila, Alex</dc:creator>
	<dc:subject xml:lang="en">Balinese grapes</dc:subject>
	<dc:subject xml:lang="en">pancreatic β cells</dc:subject>
	<dc:subject xml:lang="en">creatine kinase</dc:subject>
	<dc:subject xml:lang="en">overtraining.</dc:subject>
	<dc:description xml:lang="en">Overtraining is a high-volume, high-intensity, long-duration, and high-frequency training. The heavier physical training, the more oxygen needed for metabolism. Increasing oxygen usage causes an elevation of electron leakage in mitochondria;thus,produce a higher amount of reactive oxygen species (ROS). Antioxidant inhibits oxidative damage in a target molecule. Grapes contain a lot of antioxidants, such as polyphenols and anthocyanins. The purpose of this study was to examine the effect of ethanol extract of Balinese grapes (Vitis vinifera) on β-cells and muscle damage in overtraining-induced rats. This study was a completely randomized experimental study using a posttest only control group design. Samples were 36 male albino rats (Rattus norvergicus), aged 2.5-3 months, divided randomly into two groups. The control group (P0), 18 rats, were given overtraining and placebo of 2 ml distilled water; the treatment group (P1) 18 rats, were given overtraining and 25g/kg.BW ethanol extract of Balinese grapes. The result showed the average amount of pancreatic β-cells in the P0 group was higher than the P1 group (p&amp;lt; 0.001). In addition, the average level of creatine kinase was also higher in the P1 group than those of the P0 group (p&amp;lt;0,001). It can be concluded that the administration of ethanolic extract of Balinese grapes mitigates the damage on pancreatic β-cells and muscle cells induced with overtraining.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2020-11-05</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/jrvi/article/view/9688</dc:identifier>
	<dc:source xml:lang="en">Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research); VOLUME 4 No. 2, JULY 2020</dc:source>
	<dc:source>2615-2835</dc:source>
	<dc:source>2614-0187</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/jrvi/article/view/9688/6014</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research)</dc:rights>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>