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Identification of Pseudomonas fluorescens using different biochemical tests

International Journal of Applied Biology · Vol. 2 No. 2 (2018) · pp. 27–32

ISSN
2580-2119, 2580-2410
Published
2018-12-25
Publisher
Hasanuddin University
Language
en

Abstract

Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P. fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens per seed which is necessary for better seedling growth and effective biological control of pathogens.

Collaboration network of the authors

20 authors · 37 co-authorship links

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Co-authorOrganisationJoint articles
Pitamber ThakurAffiliation unknown1
Ghanashyam BhandariAffiliation unknown1
Buddhi Bahadur AchhamiAffiliation unknown1
Dil Bahadur GurungAffiliation unknown1
Balram BhandariAffiliation unknown1
Mahendra Prasad TripathiAffiliation unknown1
Bhim Nath AdhikariAffiliation unknown1
Bishal DhakalAffiliation unknown1
Bishnu Prasad JoshiAffiliation unknown1
Naba Raj BhattaAffiliation unknown1
Saroj ThapaAffiliation unknown1
Khagendra ThapaAffiliation unknown1
Amit ChaudharyAffiliation unknown1
Bishnu Hari AdhikaryAffiliation unknown1
Bandhu Raj BaralAffiliation unknown1
Manish Kumar PalAffiliation unknown1
Kapil KafleAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ijoab/oai, first harvested 3 Nov 2026, last seen 3 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Identification of Pseudomonas fluorescens using different biochemical tests</dc:title>
	<dc:creator xml:lang="en">Nepali, Bikram</dc:creator>
	<dc:creator xml:lang="en">Bhattarai, Sabin</dc:creator>
	<dc:creator xml:lang="en">Shrestha, Jiban</dc:creator>
	<dc:description xml:lang="en">Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P.  fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens  per seed which is necessary for better seedling growth and effective biological control of pathogens.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2018-12-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ijoab/article/view/5260</dc:identifier>
	<dc:source xml:lang="en">International Journal of Applied Biology; Vol. 2 No. 2 (2018): International Journal of Applied Biology; 27-32</dc:source>
	<dc:source>2580-2119</dc:source>
	<dc:source>2580-2410</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ijoab/article/view/5260/3180</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 International Journal of Applied Biology</dc:rights>
</oai_dc:dc>