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EXPLORATION OF INDIGENOUS YEASTS AS INOCULUM IN FERMENTATION OF SUGAR FACTORY WASTE (BAGASSE) INTO XYLITOL LOW-CALORIE SUGAR

International Journal of Applied Biology · Vol. 5 No. 2 (2021) · pp. 175–189

ISSN
2580-2119, 2580-2410
Published
2021-12-31
Publisher
Hasanuddin University
Language
en

Abstract

Xylitol is a pentose sugar that has many benefits. The production of xylitol in biotechnology is more promising because in its production it only utilizes xylose fermentative yeast. This study aims to obtain yeast isolates and determine their ability to ferment xylose to xylitol. The samples used came from palm sap, coconut sap, soil and sugar press-mud. Yeast isolation was carried out by growing on YMA media which had added 0.1% chloramphenicol. Subsequently, the colony and cell morphology were observed. A total of 12 isolates of palm sap, 12 isolates of coconut sap, 8 isolates from soil and 6 isolates from sugar press-mud were screened on YPX Agar and Xylose Broth media with 3% xylose concentration to see their ability to grow on xylose media. Isolates P1, S3, KP3, KP4, T4 and B4 were selected for the fermentation test because they had the best growth in xylose broth as indicated by the highest OD value. Fermentation was carried out for 72 hours by measuring the pH value, total yeast at intervals of 24 hours. The xylitol levels formed were measured using HPLC/UPLC. Based on the measurement of the xylitol levels formed, the results were obtained in isolates P1 which was 1.28 g/100mL, S3 was 2.05 g/100mL, KP3 was 2.23 g/100mL, KP4 was 2.19 g/100mL, T4 2 was g /100mL and B4 2.47 g/100 mL with xylitol yields P1 which is 0.59 g/g, S3 is 0.67 g/g, KP3 is 0.63 g/g, KP4 is 0.62 g/g, T4 is 0.66 g/g and B4 were 0.64 g/g so that the six isolates isolated from palm sap, coconut sap, soil and sugar press-mud had the ability to produce xylitol.

Keywords

Collaboration network of the authors

39 authors · 154 co-authorship links

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  • Sunan Kalijaga State Islamic University Yogyakarta
  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Fuad GaniAffiliation unknown3
Nur Umriani PermatasariAffiliation unknown2
As'adi AbdullahAffiliation unknown2
Magdalena LitaayAffiliation unknown2
Zaraswati DwayanaAffiliation unknown1
Eva JohanesAffiliation unknown1
Ruslan UmarAffiliation unknown1
Syafrian Nur MuhammadAffiliation unknown1
Nur Afifah ZhafirahAffiliation unknown1
Paulina TabaAffiliation unknown1
Nur AfniAffiliation unknown1
Djabal Nur BasirAffiliation unknown1
Bulkis MusaAffiliation unknown1
Prayiska Mariana RapiAffiliation unknown1
Emmi AstutiAffiliation unknown1
Novi LestariAffiliation unknown1
Nurmala SariAffiliation unknown1
Nur Husnul KhotimahAffiliation unknown1
HeriadiAffiliation unknown1
Fahruddin FahruddinSunan Kalijaga State Islamic University Yogyakarta1
Abdul WahabAffiliation unknown1
Rifaat RifaatAffiliation unknown1
Syahribulan SyahribulanAffiliation unknown1
Dody PriosambodoAffiliation unknown1
Erviana TahirAffiliation unknown1
Risco G BudjiAffiliation unknown1
Karlina SariAffiliation unknown1
Risco B GobelAffiliation unknown1
Eva JohannesAffiliation unknown1
Henra -Affiliation unknown1
Abdul SalamInstitut Agama Islam Negeri Kendari1
Zaraswati DwyanaAffiliation unknown1
Mustika TuwoAffiliation unknown1
Muhammad Ruslan UmarAffiliation unknown1
Nur Umriani P UmarAffiliation unknown1
Nenis SardianiAffiliation unknown1
Mutia Putri JamaluddinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ijoab/oai, first harvested 3 Nov 2026, last seen 3 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">EXPLORATION OF INDIGENOUS YEASTS AS INOCULUM IN FERMENTATION OF SUGAR FACTORY WASTE (BAGASSE) INTO XYLITOL LOW-CALORIE SUGAR</dc:title>
	<dc:creator xml:lang="en">Haedar, Nur</dc:creator>
	<dc:creator xml:lang="en">Heriadi, Heriadi</dc:creator>
	<dc:subject xml:lang="en">Yeast</dc:subject>
	<dc:subject xml:lang="en">Xylitol </dc:subject>
	<dc:subject xml:lang="en">Sap</dc:subject>
	<dc:subject xml:lang="en">soil</dc:subject>
	<dc:subject xml:lang="en">press-mud</dc:subject>
	<dc:description xml:lang="en">Xylitol is a pentose sugar that has many benefits. The production of xylitol in biotechnology is more promising because in its production it only utilizes xylose fermentative yeast. This study aims to obtain yeast isolates and determine their ability to ferment xylose to xylitol. The samples used came from palm sap, coconut sap, soil and sugar press-mud. Yeast isolation was carried out by growing on YMA media which had added 0.1% chloramphenicol. Subsequently, the colony and cell morphology were observed. A total of 12 isolates of palm sap, 12 isolates of coconut sap, 8 isolates from soil and 6 isolates from sugar press-mud were screened on YPX Agar and Xylose Broth media with 3% xylose concentration to see their ability to grow on xylose media. Isolates P1, S3, KP3, KP4, T4 and B4 were selected for the fermentation test because they had the best growth in xylose broth as indicated by the highest OD value. Fermentation was carried out for 72 hours by measuring the pH value, total yeast at intervals of 24 hours. The xylitol levels formed were measured using HPLC/UPLC. Based on the measurement of the xylitol levels formed, the results were obtained in isolates P1 which was 1.28 g/100mL, S3 was 2.05 g/100mL, KP3 was 2.23 g/100mL, KP4 was 2.19 g/100mL, T4 2 was g /100mL and B4 2.47 g/100 mL with xylitol yields P1 which is 0.59 g/g, S3 is 0.67 g/g, KP3 is 0.63 g/g, KP4 is 0.62 g/g, T4 is 0.66 g/g and B4 were 0.64 g/g so that the six isolates isolated from palm sap, coconut sap, soil and sugar press-mud had the ability to produce xylitol.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2021-12-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ijoab/article/view/18829</dc:identifier>
	<dc:source xml:lang="en">International Journal of Applied Biology; Vol. 5 No. 2 (2021): International Journal of Applied Biology; 175-189</dc:source>
	<dc:source>2580-2119</dc:source>
	<dc:source>2580-2410</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ijoab/article/view/18829/7730</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 International Journal of Applied Biology</dc:rights>
</oai_dc:dc>