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Jurnal

Fish Protein Hydrolyzate From Grouper Epinephelus Offal Using Bromelain Enzyme Pineapple Crude Extract

International Journal of Applied Biology · Vol. 6 No. 2 (2022) · pp. 222–229

ISSN
2580-2119, 2580-2410
Published
2022-12-29
Publisher
Hasanuddin University
Language
en

Abstract

Grouper is one of the demersal fish groups that are abundant in Indonesian marine waters. In addition, the high market demand for this fish has also increased its main production technique as an export product. One example of a product from this fish is fish fillet which still leaves waste. Offal is an example of a by-product of grouper which is underutilized so that it is of low value in the market and as a resource. The high protein content of fish offal can be used as a raw material in the manufacture of protein hydrolysates which of course require the role of proteolytic enzymes. Bromelain enzyme which is a proteolytic enzyme that is protease. This study aims to determine the effect of the bromelain enzyme concentration obtained from pineapple crude extract on the quality of the protein hydrolyzate of grouper offal. The experimental design used in this study was a Factorial complete randomized design with different enzyme concentrations (4, 6, 8, 10). and 12%) and the difference in water bath time (4, 6, 8, and 10 hours). The results showed the bromelain enzyme concentration had a very significant effect on the yield and degree of hydrolysis of snapper offal, and observations of the proximate results showed the potential utilization of hydrolyzed protein of fish offal as a food source.

Keywords

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24 authors · 83 co-authorship links

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Co-authorOrganisationJoint articles
. SyahrulAffiliation unknown2
Asyhabul QaffiAffiliation unknown1
Wahdanial FitriAffiliation unknown1
Mufti Hatur Rahmi TahirAffiliation unknown1
Fahrul fahrulAffiliation unknown1
Elmi N. ZainuddinAffiliation unknown1
Syahrul SyahrulAffiliation unknown1
Metusalach MetusalachAffiliation unknown1
Kasmiati KasmiatiAffiliation unknown1
Ophirtus SumuleAffiliation unknown1
Mufti Hatur RahmiAffiliation unknown1
Ryan Ma’rup RamadhanAffiliation unknown1
Alisha Shafa AzzahraAffiliation unknown1
INA SUNDARIAffiliation unknown1
Jecly PaembonanAffiliation unknown1
Nur Fauzi ZaahirahAffiliation unknown1
Muh Naufal BissawabAffiliation unknown1
Kevin Putra SenawiAffiliation unknown1
Syeli Novita Putri EmbutatobaAffiliation unknown1
Nur Resky FebriantiAffiliation unknown1
KasmiatiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ijoab/oai, first harvested 3 Nov 2026, last seen 3 Nov 2026.
Original metadata (oai_dc)
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	<dc:title xml:lang="en">Fish Protein Hydrolyzate From Grouper Epinephelus Offal Using Bromelain Enzyme Pineapple Crude Extract</dc:title>
	<dc:creator xml:lang="en">Palla, Andi Nur Faiziah</dc:creator>
	<dc:creator xml:lang="en">Metusalach</dc:creator>
	<dc:creator xml:lang="en">Nursinah Amir</dc:creator>
	<dc:subject xml:lang="en">Grouper Fish</dc:subject>
	<dc:subject xml:lang="en">Enzyme bromelain</dc:subject>
	<dc:subject xml:lang="en">Protein Hydrolyzate</dc:subject>
	<dc:description xml:lang="en">Grouper is one of the demersal fish groups that are abundant in Indonesian marine waters. In addition, the high market demand for this fish has also increased its main production technique as an export product. One example of a product from this fish is fish fillet which still leaves waste. Offal is an example of a by-product of grouper which is underutilized so that it is of low value in the market and as a resource. The high protein content of fish offal can be used as a raw material in the manufacture of protein hydrolysates which of course require the role of proteolytic enzymes. Bromelain enzyme which is a proteolytic enzyme that is protease. This study aims to determine the effect of the bromelain enzyme concentration obtained from pineapple crude extract on the quality of the protein hydrolyzate of grouper offal. The experimental design used in this study was a Factorial complete randomized design with different enzyme concentrations (4, 6, 8, 10). and 12%) and the difference in water bath time (4, 6, 8, and 10 hours). The results showed the bromelain enzyme concentration had a very significant effect on the yield and degree of hydrolysis of snapper offal, and observations of the proximate results showed the potential utilization of hydrolyzed protein of fish offal as a food source.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2022-12-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ijoab/article/view/24091</dc:identifier>
	<dc:source xml:lang="en">International Journal of Applied Biology; Vol. 6 No. 2 (2022): International Journal of Applied Biology; 222-229</dc:source>
	<dc:source>2580-2119</dc:source>
	<dc:source>2580-2410</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ijoab/article/view/24091/9103</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 International Journal of Applied Biology</dc:rights>
</oai_dc:dc>