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EFFECTS OF SONICATION DURATION AND CONCENTRATION OF NEEM-BASED ECO-ENZYMES ON Spodoptera litura MORTALITY

Jurnal Ilmiah Ilmu Terapan Universitas Jambi · Vol. 10 No. 5 (2026) · hlm. 2549–2555

DOI
10.22437/jiituj.v10i5.58876
ISSN
2580-2259, 2580-2240
Terbit
2026-10-31
Penerbit
LPPM Universitas Jambi
Bahasa
en
Lisensi
creativecommons.org/licenses/by/4.0

Abstrak

Spodoptera litura is a major agricultural pest causing substantial crop losses. Neem-based eco-enzymes produced through organic fermentation contain bioactive compounds with potential insecticidal activity. This study investigated how sonication duration and eco-enzyme concentration affected S. litura mortality and estimated the Lethal Concentration (LC₅₀) and Lethal Time (LT₅₀). This study systematically evaluated combined sonication durations and concentrations of neem-based eco-enzyme formulations through topical and oral bioassays, enabling comparison of insecticidal performance across exposure routes. A completely randomized design comprised 12 treatments: 9 combinations of 3 sonication durations (3, 5, and 7 h) and 3 concentrations (3%, 5%, and 7%), a 20% non-sonicated eco-enzyme treatment, a control treatment, and a chemical insecticide treatment. Each treatment included 3 replications with 20 third-instar larvae per experimental unit. For each bioassay, we randomly selected 720 larvae from 800 eligible larvae. We recorded mortality at predetermined intervals over 7 days. Data were analyzed using analysis of variance, followed by Duncan’s Multiple Range Test at the 5% significance level. We estimated LC₅₀ and LT₅₀ using probit analysis. The 3-hour sonication treatment at 7% concentration produced the highest mortality, reaching 81.67% in the topical assay and 80.00% in the oral assay after 7 days. These findings identify a promising formulation and provide comparative evidence of its activity through two application routes. The estimated LC₅₀ and LT₅₀ were approximately 2% and 3 h, respectively. Further studies should characterize physicochemical properties and validate efficacy under field conditions.

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  • Universitas Jember
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  • Universitas Pekalongan
  • Magyar Agrár- és Élettudományi Egyetem
  • National Research and Innovation Agency
  • Islamic University of Indonesia
  • Universitas Borneo Tarakan
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Rekan penulisOrganisasiArtikel bersama
Fariz Kustiawan AlfarisyUniversitas Jember5
Ankardiansyah Pandu PradanaUniversitas Jember3
Rachmi MasnilahUniversitas Jember2
Farchan Mushaf Al RamadhaniUniversitas Pekalongan2
Nanang Tri HaryadiUniversitas Jember2
Sri WahyuningsihUniversitas Jember2
Aryo Fajar SunatomoAfiliasi tidak diketahui1
Gusna MerinaAfiliasi tidak diketahui1
Farchan Mushal Al RamadhanAfiliasi tidak diketahui1
RismayaniAfiliasi tidak diketahui1
Suhartiningsih Dwi NurchayantiUniversitas Jember1
Dwi Tirta SariUniversitas Jember1
Sri Ita TariganMagyar Agrár- és Élettudományi Egyetem1
Sri HartatikUniversitas Jember1
Mohammad UbaidillahUniversitas Jember1
Ihsan HabibiUniversitas Jember1
Angger Aisyah Hadiahning GustiUniversitas Jember1
Rosalina Diva AurelaUniversitas Jember1
Rikno HarmokoNational Research and Innovation Agency1
Imron RosyidiUniversitas Jember1
Hari PurnomoIslamic University of Indonesia1
Muh. AdiwenaUniversitas Borneo Tarakan1
Anita FirnandaUniversitas Jember1
Siswoyo SoekarnoUniversitas Jember1

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Sumber metadata: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/JIITUJ/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">EFFECTS OF SONICATION DURATION AND CONCENTRATION OF NEEM-BASED ECO-ENZYMES ON Spodoptera litura MORTALITY</dc:title>
	<dc:creator xml:lang="en">Zuhro, Fatimatuz</dc:creator>
	<dc:creator xml:lang="en">Suharto, Suharto</dc:creator>
	<dc:creator xml:lang="en">Hoesain, Mohammad</dc:creator>
	<dc:creator xml:lang="en">Hariyono, Kacung</dc:creator>
	<dc:subject xml:lang="en">Eco-Enzymes</dc:subject>
	<dc:subject xml:lang="en">Mortality</dc:subject>
	<dc:subject xml:lang="en">Nanoformulation</dc:subject>
	<dc:subject xml:lang="en">Sonication</dc:subject>
	<dc:subject xml:lang="en">S. litura</dc:subject>
	<dc:description xml:lang="en">Spodoptera litura is a major agricultural pest causing substantial crop losses. Neem-based eco-enzymes produced through organic fermentation contain bioactive compounds with potential insecticidal activity. This study investigated how sonication duration and eco-enzyme concentration affected S. litura mortality and estimated the Lethal Concentration (LC₅₀) and Lethal Time (LT₅₀). This study systematically evaluated combined sonication durations and concentrations of neem-based eco-enzyme formulations through topical and oral bioassays, enabling comparison of insecticidal performance across exposure routes. A completely randomized design comprised 12 treatments: 9 combinations of 3 sonication durations (3, 5, and 7 h) and 3 concentrations (3%, 5%, and 7%), a 20% non-sonicated eco-enzyme treatment, a control treatment, and a chemical insecticide treatment. Each treatment included 3 replications with 20 third-instar larvae per experimental unit. For each bioassay, we randomly selected 720 larvae from 800 eligible larvae. We recorded mortality at predetermined intervals over 7 days. Data were analyzed using analysis of variance, followed by Duncan’s Multiple Range Test at the 5% significance level. We estimated LC₅₀ and LT₅₀ using probit analysis. The 3-hour sonication treatment at 7% concentration produced the highest mortality, reaching 81.67% in the topical assay and 80.00% in the oral assay after 7 days. These findings identify a promising formulation and provide comparative evidence of its activity through two application routes. The estimated LC₅₀ and LT₅₀ were approximately 2% and 3 h, respectively. Further studies should characterize physicochemical properties and validate efficacy under field conditions.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2026-10-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/JIITUJ/article/view/58876</dc:identifier>
	<dc:identifier>10.22437/jiituj.v10i5.58876</dc:identifier>
	<dc:source xml:lang="en">Jurnal Ilmiah Ilmu Terapan Universitas Jambi; Vol. 10 No. 5 (2026): Volume 10, Nomor 5, October 2026; 2549-2555</dc:source>
	<dc:source>2580-2259</dc:source>
	<dc:source>2580-2240</dc:source>
	<dc:source>10.22437/jiituj.v10i5</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/JIITUJ/article/view/58876/25981</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Fatimatuz Zuhro, Suharto, Mohammad Hoesain, Kacung Hariyono</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>