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PRODUCTION OF PROTEASE ENZYME FROM BACTERIA IN HOT SPRING OF SOUTH SULAWESI, Bacillus licheniformis HSA3-1a

Marina Chimica Acta · Vol. 16 No. 1 (2015)

ISSN
1411-2132
Published
2015-04-01
Publisher
Hasanuddin University
Language
en

Abstract

Bacillus licheniformis HSA3-1a is able to produce extracellular protease. The aim of this research was to find out its production optimum time, consentration of MnCl2, temperature and optimum pH, also the cofactor influence on activity protease from B. licheniformis HSA3-1a. The protease activity was tested with a modified method of Walter. Protease production time was optimum for 36 hours with an activity value of 0,394 U/mL, protein content was 132,35 mg/mL. The Optimum condition of protease enzyme were pH 7,0 and temperature 45 ⁰C with an activity value of 0,0463 U/mL. Protease activity could be inhibited by MnCl2 concentration of 0,01 - 0,03 M.

Collaboration network of the authors

33 authors · 85 co-authorship links

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  • Universitas Muhammadiyah Buton
  • Sunan Kalijaga State Islamic University Yogyakarta
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Seniwati DaliAffiliation unknown7
Ahyar AhmadAffiliation unknown4
Abdur Rahman ArifAffiliation unknown3
Muawanah MuawanahAffiliation unknown2
Sartika DanialAffiliation unknown2
Rugaiyah A ArfahAffiliation unknown1
Muhammad ZakirAffiliation unknown1
St Fauziah St FauziahAffiliation unknown1
Prastawa BudiAffiliation unknown1
Ischaidar IschaidarAffiliation unknown1
Indah RayaAffiliation unknown1
Erna MayasariAffiliation unknown1
Hanapi UsmanAffiliation unknown1
Paulina TabaAffiliation unknown1
Fatahu FatahuAffiliation unknown1
Firdaus FirdausAffiliation unknown1
Nunuk Hariani SoekamtoAffiliation unknown1
Selfi WullurAffiliation unknown1
Leliani LelianiAffiliation unknown1
Leliani RamliAffiliation unknown1
Alfian Nasir MaidinAffiliation unknown1
ABDUL KARIMAffiliation unknown1
Sulfiani SulfianiAffiliation unknown1
Lupita Denta PutriAffiliation unknown1
Fitriani BUniversitas Muhammadiyah Buton1
Nur AsmiAffiliation unknown1
Harningsih KarimAffiliation unknown1
Ali MuhakimAffiliation unknown1
Siti KhairunnurAffiliation unknown1
Fahruddin FahruddinSunan Kalijaga State Islamic University Yogyakarta1
Nur HaedarAffiliation unknown1
Wilda AryantiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/mca/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">PRODUCTION OF PROTEASE ENZYME FROM BACTERIA IN HOT SPRING OF SOUTH SULAWESI, Bacillus licheniformis HSA3-1a</dc:title>
	<dc:creator xml:lang="en">Natsir, Hasnah</dc:creator>
	<dc:description xml:lang="en">Bacillus licheniformis HSA3-1a is able to produce extracellular protease. The aim of this research was to find out its production optimum time, consentration of MnCl2, temperature and optimum pH, also the cofactor influence on activity protease from B. licheniformis HSA3-1a. The protease activity was tested with a modified method of Walter. Protease production time was optimum for 36 hours with an activity value of 0,394 U/mL, protein content was 132,35 mg/mL. The Optimum condition of protease enzyme were pH 7,0 and temperature 45 ⁰C with an activity value of 0,0463 U/mL. Protease activity could be inhibited by MnCl2 concentration of 0,01 - 0,03 M.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2015-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/mca/article/view/949</dc:identifier>
	<dc:source xml:lang="en">Marina Chimica Acta; Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age</dc:source>
	<dc:source>1411-2132</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/mca/article/view/949/606</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2016 Marina Chimica Acta</dc:rights>
</oai_dc:dc>