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MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS

Marina Chimica Acta · Vol. 16 No. 1 (2015)

ISSN
1411-2132
Published
2015-04-01
Publisher
Hasanuddin University
Language
en

Abstract

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules

Collaboration network of the authors

40 authors · 105 co-authorship links · showing the 40 most connected

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  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Maming GaffarAffiliation unknown14
Muhammad ZakirAffiliation unknown11
Tjodi HarlimAffiliation unknown7
Maming MamingAffiliation unknown6
Ahyar AhmadAffiliation unknown6
Muhammad LukmanAffiliation unknown5
Nicole de VoogdAffiliation unknown4
Asma AssaAffiliation unknown4
Ajuk SaparAffiliation unknown3
Nunuk Hariani SoekamtoAffiliation unknown3
Lydia MelawatyAffiliation unknown3
Rakhmad ArmusAffiliation unknown3
Paulina TabaAffiliation unknown2
Yulia Yusrini DjabirAffiliation unknown2
Elly WahyudinAffiliation unknown2
Tri Aryono HadiAffiliation unknown2
Adiba AriefAffiliation unknown2
Waode RustiahAffiliation unknown2
Musa RamangAffiliation unknown2
Saefuddin .Affiliation unknown1
Ahmad ZainiAffiliation unknown1
ZarlinaAffiliation unknown1
Agus Sangka PratamaAffiliation unknown1
M Nasrum MassiAffiliation unknown1
Marianti A. ManggauAffiliation unknown1
Hasyim KasimAffiliation unknown1
Subehan LalloAffiliation unknown1
Restuyani ParanoanAffiliation unknown1
Sartini SartiniAffiliation unknown1
Munawarah MunawarahAffiliation unknown1
Arif SantosoAffiliation unknown1
Nur IdaInstitut Agama Islam Negeri Kendari1
MusdalifahAffiliation unknown1
Muh. Nasrum MassiAffiliation unknown1
Muhammad Subhan A. SibaduAffiliation unknown1
Nurul Muhlisa MusAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/mca/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS</dc:title>
	<dc:creator xml:lang="en">Marzuki, Ismail</dc:creator>
	<dc:creator xml:lang="en">Noor, Alfian</dc:creator>
	<dc:creator xml:lang="en">Nafie, Nursiah La</dc:creator>
	<dc:creator xml:lang="en">Djide, M Natsir</dc:creator>
	<dc:description xml:lang="en">Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2015-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/mca/article/view/956</dc:identifier>
	<dc:source xml:lang="en">Marina Chimica Acta; Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age</dc:source>
	<dc:source>1411-2132</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/mca/article/view/956/610</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2016 Marina Chimica Acta</dc:rights>
</oai_dc:dc>