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ANALYSIS OF Fe, Zn, AND Cr AT SOME FRACTIONATED PROTEINS IN SPONGE AGELAS NAKAMURAI

Marina Chimica Acta · Vol. 15 No. 2 (2016)

ISSN
1411-2132
Published
2016-08-28
Publisher
Hasanuddin University
Language
en

Abstract

This research aims to analyze the concentration of metals Fe, Zn, and Cr in protein fractions of sponge Agelas nakamurai from Kapoposang Island, Spermonde archipelago. Protein samples were extracted, fractionated using various saturated ammonium sulphate (SAS) concentration, namely 0-20%, 20-40%, 40-60% and 60-80%, then purified by dialysis using celophane membranes. Each fraction was digested using microwave before metal analysis using ICP OES (Inductively Coupled Plasma Optical Emission Spectroscopy). The results have shown that highest iron concentration was found in 20-40% fraction, 28.2 µg/L, while lowest one was in 60-80% fraction, 2.39 µg/L. For Zn, its highest concentration were found 20-40% fraction, 9.42 µg/L and the lowest one was 40-60%, 3.66 µg/L. On the other hand, the concentration of Cr was found highest at 20-40% fraction, 2.28 µg/L, while its lowest amount was at 60-80% fraction, 0.238 µg/L. In the meantime, its high- est antibacterial activity was at 0-20% fraction examined in Salmonella thypusa with inhibition diameter was 7,00 mm, where the amount of essential metals Fe, Zn and Cr are 17,2 µg/L, 6,32 µg/L, 1,47 µg/L. It was concluded that the essential metals investigated were coexisted and distrib- uted in several protein fractions and its bioactive properties.Keywords: Agelas nakamurai, Bioactive protein, essential metals, ICP-OES, Kapoposang Island

Collaboration network of the authors

40 authors · 99 co-authorship links · showing the 40 most connected

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  • Tadulako University
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Muhammad ZakirAffiliation unknown16
Tjodi HarlimAffiliation unknown6
Maming MamingAffiliation unknown6
Ahyar AhmadAffiliation unknown6
Muhammad LukmanAffiliation unknown6
Nursiah La NafieAffiliation unknown4
Nicole de VoogdAffiliation unknown4
Ajuk SaparAffiliation unknown3
Nunuk Hariani SoekamtoAffiliation unknown3
Lydia MelawatyAffiliation unknown3
Rakhmad ArmusAffiliation unknown3
Waode RustiahAffiliation unknown3
Paulina TabaAffiliation unknown2
Ismail MarzukiAffiliation unknown2
Tri Aryono HadiAffiliation unknown2
Asma AssaAffiliation unknown2
Waode Nur RahmaniahAffiliation unknown2
Rahmawati RahmawatiAffiliation unknown2
Usman UsmanAffiliation unknown2
Saefuddin .Affiliation unknown1
Ahmad ZainiAffiliation unknown1
ZarlinaAffiliation unknown1
Ahmad FaizalAffiliation unknown1
Shinta WerorilangiAffiliation unknown1
Akbar TahirAffiliation unknown1
M. Farid SamawiAffiliation unknown1
Rahmawati RahmawatiAffiliation unknown1
M Natsir DjideAffiliation unknown1
Febby KurniatyAffiliation unknown1
Suhaela SuhaelaTadulako University1
Hafiludin UliAffiliation unknown1
Fredryk Welliam MandeyAffiliation unknown1
Sukmawati SukmawatiAffiliation unknown1
Firdaus ZentaAffiliation unknown1
Musa RamangAffiliation unknown1
Gita Permata SariAffiliation unknown1
Syarifuddin SyarifuddinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/mca/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">ANALYSIS OF  Fe, Zn, AND Cr AT SOME FRACTIONATED PROTEINS IN SPONGE AGELAS NAKAMURAI</dc:title>
	<dc:creator xml:lang="en">Ramadhani, Anita Nur</dc:creator>
	<dc:creator xml:lang="en">Noor, Alfian</dc:creator>
	<dc:creator xml:lang="en">Gaffar, Maming</dc:creator>
	<dc:description xml:lang="en">This research aims to analyze the concentration of metals Fe, Zn, and Cr in protein fractions of sponge Agelas nakamurai from Kapoposang Island, Spermonde archipelago. Protein samples were extracted, fractionated using various saturated ammonium sulphate (SAS) concentration, namely 0-20%, 20-40%, 40-60% and 60-80%, then purified by dialysis using celophane membranes. Each fraction was digested using microwave before metal analysis using ICP OES (Inductively Coupled Plasma Optical Emission Spectroscopy). The results have shown that highest iron concentration was found in 20-40% fraction, 28.2 µg/L, while lowest one was in 60-80% fraction, 2.39 µg/L. For Zn, its highest concentration were found 20-40% fraction,  9.42 µg/L and the lowest one was 40-60%, 3.66 µg/L. On the other hand, the concentration of Cr was found highest at 20-40% fraction, 2.28 µg/L, while its lowest amount was at  60-80% fraction, 0.238 µg/L. In the meantime, its high- est antibacterial activity was at 0-20% fraction examined in Salmonella thypusa with inhibition diameter was 7,00 mm, where the amount of essential metals Fe, Zn and Cr are 17,2 µg/L, 6,32 µg/L, 1,47 µg/L. It was concluded that the essential metals investigated were coexisted and distrib- uted in several protein fractions and its bioactive properties.Keywords   :  Agelas  nakamurai,  Bioactive  protein,  essential  metals, ICP-OES,  Kapoposang Island</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2016-08-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/mca/article/view/961</dc:identifier>
	<dc:source xml:lang="en">Marina Chimica Acta; Vol. 15 No. 2 (2014): Metals in Marine Samples and Age Determination using Liquid Scintillation Counting</dc:source>
	<dc:source>1411-2132</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/mca/article/view/961/613</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2016 Marina Chimica Acta</dc:rights>
</oai_dc:dc>