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PURIFICATION, ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE FRACTION FROM RED ALGAE Gracilaria verrucosa

Marina Chimica Acta · Vol. 18 No. 2 (2017)

ISSN
1411-2132
Published
2017-11-21
Publisher
Hasanuddin University
Language
en

Abstract

it contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity of polysaccharide extract and fraction fromred algae Gracilariaverrucosa. The study was carried out by isolating the polysaccharide extract using aqueous 100 o C and methanol-ethanol precipitation.Purification of polysaccharide done by Column Chromatography DEAE-Cellulose and analysis polysaccharide concentration was using phenol-sulfuric acid. Antioxidant activity of crude extract and polysaccharide fraction was examined using DPPH method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results reveals that the highest polysaccharide concentration was fraction 7 with value of 0,267 mg/mL, and the crude extract and fraction 4 have strongest antioxidant activity with IC50 values of 51,22 and 62,25 ppm, respectively. The result of BSLT assay showed that the crude extract and fraction 6 has a highest toxicity with LC50 values of 47,73and 58,48 ppm, therefore it is classified as toxic. The polysaccharide of crude extract and fraction 7 has a potential to be developed as an alternative antioxidant and antitumor agent. Identification with FT-IR shows the characteristic bands of agarocolloid.

Collaboration network of the authors

40 authors · 98 co-authorship links · showing the 40 most connected

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  • Tadulako University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Seniwati DaliAffiliation unknown9
Alfian NoorAffiliation unknown6
Abdur Rahman ArifAffiliation unknown3
Muhammad LukmanAffiliation unknown3
Rakhmad ArmusAffiliation unknown3
Hanapi UsmanAffiliation unknown3
Sartika DanialAffiliation unknown2
Muh. Nasrum MassiAffiliation unknown2
Rugaiyah A ArfahAffiliation unknown1
Muhammad ZakirAffiliation unknown1
St Fauziah St FauziahAffiliation unknown1
Prastawa BudiAffiliation unknown1
Baharuddin PatandjengiAffiliation unknown1
Tutik KuswinantiAffiliation unknown1
Irene BrugmanAffiliation unknown1
Nurmujahidin NurmujahidinAffiliation unknown1
Ischaidar IschaidarAffiliation unknown1
Suhaela SuhaelaTadulako University1
Indah RayaAffiliation unknown1
Erna MayasariAffiliation unknown1
Ajuk SaparAffiliation unknown1
Nunuk Hariani SoekamtoAffiliation unknown1
Tri Aryono HadiAffiliation unknown1
Mahdalia MahmudAffiliation unknown1
Eva JohannesAffiliation unknown1
Nur UmrianiAffiliation unknown1
Abdul Rauf PatongAffiliation unknown1
Paulina TabaAffiliation unknown1
Fatahu FatahuAffiliation unknown1
Firdaus FirdausAffiliation unknown1
Nunuk Hariani SoekamtoAffiliation unknown1
Selfi WullurAffiliation unknown1
Leliani LelianiAffiliation unknown1
Leliani RamliAffiliation unknown1
Mashuni MashuniAffiliation unknown1
Abdul Wahid WahabAffiliation unknown1
Muhammad SyahrulAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/mca/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">PURIFICATION, ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE FRACTION FROM RED ALGAE Gracilaria verrucosa</dc:title>
	<dc:creator xml:lang="en">Muawanah, Muawanah</dc:creator>
	<dc:creator xml:lang="en">Ahmad, Ahyar</dc:creator>
	<dc:creator xml:lang="en">Natsir, Hasnah</dc:creator>
	<dc:description xml:lang="en"> it contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity of polysaccharide extract and fraction fromred algae Gracilariaverrucosa. The study was carried out by isolating the polysaccharide extract using aqueous 100 o C and methanol-ethanol precipitation .Purification of polysaccharide done by Column Chromatography DEAE-Cellulose and analysis polysaccharide concentration was using phenol-sulfuric acid. Antioxidant activity of crude extract and polysaccharide fraction was examined using DPPH method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results reveals that the highest polysaccharide concentration was fraction 7 with value of 0,267 mg/mL, and  the crude extract and fraction  4 have strongest antioxidant activity with IC50 values of 51,22 and 62,25 ppm, respectively. The result of BSLT assay showed that the crude extract and fraction 6 has a highest toxicity with LC50 values of 47,73and 58,48 ppm, therefore it is classified as toxic. The polysaccharide of crude extract and fraction 7 has a potential to be developed as an alternative antioxidant and antitumor agent. Identification with FT-IR shows the characteristic bands of agarocolloid.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2017-11-21</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/mca/article/view/2688</dc:identifier>
	<dc:source xml:lang="en">Marina Chimica Acta; Vol. 18 No. 2 (2017): Volume 18 No. 2 (2017)</dc:source>
	<dc:source>1411-2132</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/mca/article/view/2688/1413</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 Marina Chimica Acta</dc:rights>
</oai_dc:dc>