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Production and Characterization of Collagenase From Bacillus sp. 6-2 Isolated From Fish Liquid Waste

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) · Vol. ume No. 1 (2019) · pp. 58–66

DOI
10.70561/ica.v12i1.5924
ISSN
2655-6049, 2085-014X
Published
2019-05-30
Publisher
Hasanuddin University
Language
en

Abstract

Collagenases are enzyme that are able to hydrolyze native collagen into fragment collagen peptides. Collagenases and its hydrolysis products have received tremendous attention in medical and industrial applications. The present study was conducted to isolate and identify new collagenase producing bacteria from fish liquid waste, then produce and characterize collagenase. A total of 7 isolate from fish liquid waste were screened on selective medium containg 2 % collagen and its activity was confirmed by the formation of clear zone. Isolat 6-2 was positif as collagenase producer and identified as Bacillus sp. 6-2 by morphological and biochemical characteristics. The optimum fermentation time of enzyme was investigated. Collagenase crude extract was characterized by the effect of pH, temperature, and metal ions. Isolat 62 optimally produced collagenase enzyme after 30 h of incubation with activity of 0.072 U/mL and protein content of 3.768 mg/mL. The optimum pH and temperature were 7.0 and 40 oC, respectively. The enzyme was activated by 1 mM Ca2+and Mg2+, and inhibited by 1 mM Zn2+ and Co2+. Collagenase from Bacillus sp. 6-2 may have potentials for medical and industrial applications.

Keywords

Collaboration network of the authors

40 authors · 99 co-authorship links · showing the 40 most connected

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  • Universitas Muhammadiyah Buton
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Ahyar AhmadAffiliation unknown6
Abdur Rahman ArifAffiliation unknown4
Muhammad ZakirAffiliation unknown3
Leliani RamliAffiliation unknown3
Muawanah MuawanahAffiliation unknown2
Ischaidar IschaidarAffiliation unknown2
Hanapi UsmanAffiliation unknown2
ABDUL KARIMAffiliation unknown2
Alfian Nasir MaidinAffiliation unknown2
Lupita Denta PutriAffiliation unknown2
Rugaiyah A ArfahAffiliation unknown1
St Fauziah St FauziahAffiliation unknown1
Prastawa BudiAffiliation unknown1
Indah RayaAffiliation unknown1
Erna MayasariAffiliation unknown1
Nursiah La NafieAffiliation unknown1
Sabaniah Indjar GamaAffiliation unknown1
Paulina TabaAffiliation unknown1
Fatahu FatahuAffiliation unknown1
Sarwina HafidAffiliation unknown1
Firdaus FirdausAffiliation unknown1
Nunuk Hariani SoekamtoAffiliation unknown1
Selfi WullurAffiliation unknown1
Rauf PatongAffiliation unknown1
Muhammad Noor DjalaluddinAffiliation unknown1
Pirman APAffiliation unknown1
Adriani AdrianiAffiliation unknown1
Emmi AstutiAffiliation unknown1
Sulfiani SulfianiAffiliation unknown1
Nursyamsi NursyamsiAffiliation unknown1
Fitriani BUniversitas Muhammadiyah Buton1
Nur AsmiAffiliation unknown1
Harningsih KarimAffiliation unknown1
Ali MuhakimAffiliation unknown1
Siti KhairunnurAffiliation unknown1
Nur HaedarAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ica/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Production and Characterization of Collagenase From Bacillus sp. 6-2 Isolated From Fish Liquid Waste</dc:title>
	<dc:creator xml:lang="en">Danial, Sartika</dc:creator>
	<dc:creator xml:lang="en">Natsir, Hasnah</dc:creator>
	<dc:creator xml:lang="en">Dali, Seniwati</dc:creator>
	<dc:creator xml:lang="en">Leliani, Leliani</dc:creator>
	<dc:subject xml:lang="en">collagenase</dc:subject>
	<dc:subject xml:lang="en">collagen</dc:subject>
	<dc:subject xml:lang="en">fish liquid waste</dc:subject>
	<dc:subject xml:lang="en">Bacillus sp. 6-2</dc:subject>
	<dc:subject xml:lang="en">activity</dc:subject>
	<dc:description xml:lang="en">Collagenases are enzyme that are able to hydrolyze native collagen into fragment collagen peptides. Collagenases and its hydrolysis products have received tremendous attention in medical and industrial applications. The present study was conducted to isolate and identify new collagenase producing bacteria from fish liquid waste, then produce and characterize collagenase. A total of 7 isolate from fish liquid waste were screened on selective medium containg 2 % collagen and its activity was confirmed by the formation of clear zone. Isolat 6-2 was positif as collagenase producer and identified as Bacillus sp. 6-2 by morphological and biochemical characteristics. The optimum fermentation time of enzyme was investigated. Collagenase crude extract was characterized by the effect of pH, temperature, and metal ions. Isolat 62 optimally produced collagenase enzyme after 30 h of incubation with activity of   0.072 U/mL and protein content of 3.768 mg/mL. The optimum pH and temperature were 7.0 and 40 oC, respectively. The enzyme was activated by 1 mM Ca2+and  Mg2+, and inhibited by   1 mM  Zn2+ and Co2+. Collagenase from Bacillus sp. 6-2 may have potentials for medical and industrial applications.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2019-05-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ica/article/view/5924</dc:identifier>
	<dc:identifier>10.70561/ica.v12i1.5924</dc:identifier>
	<dc:source xml:lang="en">Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta); Volume 12, No.1: June 2019; 58-66</dc:source>
	<dc:source>2655-6049</dc:source>
	<dc:source>2085-014X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ica/article/view/5924/3603</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)</dc:rights>
</oai_dc:dc>