Melola Discovery

← Melola Discovery

Jurnal

ENZIMATIC PRODUCTION OF CHITOSAN FROM WASTE OF RAJUNGAN CRAB SHELL AND IT’S APLICATION IN CHOLESTEROL REDUCTION BY IN VITRO TEST

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) · Vol. ume No. 1 (2019) · pp. 25–34

DOI
10.70561/ica.v10i1.6404
ISSN
2655-6049, 2085-014X
Published
2019-05-12
Publisher
Hasanuddin University
Language
en

Abstract

This research aimed to show the percentages of the level of the cholesterol decreased by the convertion product of chitosan enzimatically through in vitro. The research method was started by the demineralization process of the sample in the form of powder of crab shells, and then continued with the de-colorization and de-proteination steps. The next step was de-asetilation enzymatically using de-acetylated chitin which had been isolated from Bacillus licheniformis HSA3-1a bacteria at 50OC for 2 hours until the chitosan was obtained. The research results indicated that the characteristics of the isolated chitosan had water content of 5.45%, ash content 1.53%, N-total of 6.99% and de-acetylation degree of 78.9%, and it showed white yellowish powder form. The results of the test of chitosan as the reducer of cholesterol level by in vitro process showed that 31.18% of the cholesterolwas decreased by 2 mg concentrate of chitosan. Beide, 0.5 mg of simvastatin (as positive control) had decreased by 58.29%.

Keywords

Collaboration network of the authors

40 authors · 99 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Muhammadiyah Buton
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Ahyar AhmadAffiliation unknown6
Abdur Rahman ArifAffiliation unknown4
Sartika DanialAffiliation unknown4
Muhammad ZakirAffiliation unknown3
Muawanah MuawanahAffiliation unknown2
Ischaidar IschaidarAffiliation unknown2
Hanapi UsmanAffiliation unknown2
Leliani LelianiAffiliation unknown2
Leliani RamliAffiliation unknown2
ABDUL KARIMAffiliation unknown2
Lupita Denta PutriAffiliation unknown2
Rugaiyah A ArfahAffiliation unknown1
St Fauziah St FauziahAffiliation unknown1
Prastawa BudiAffiliation unknown1
Indah RayaAffiliation unknown1
Erna MayasariAffiliation unknown1
Nursiah La NafieAffiliation unknown1
Sabaniah Indjar GamaAffiliation unknown1
Paulina TabaAffiliation unknown1
Fatahu FatahuAffiliation unknown1
Sarwina HafidAffiliation unknown1
Firdaus FirdausAffiliation unknown1
Nunuk Hariani SoekamtoAffiliation unknown1
Selfi WullurAffiliation unknown1
Rauf PatongAffiliation unknown1
Muhammad Noor DjalaluddinAffiliation unknown1
Pirman APAffiliation unknown1
Adriani AdrianiAffiliation unknown1
Emmi AstutiAffiliation unknown1
Sulfiani SulfianiAffiliation unknown1
Nursyamsi NursyamsiAffiliation unknown1
Fitriani BUniversitas Muhammadiyah Buton1
Nur AsmiAffiliation unknown1
Harningsih KarimAffiliation unknown1
Ali MuhakimAffiliation unknown1
Siti KhairunnurAffiliation unknown1
Nur HaedarAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ica/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">ENZIMATIC PRODUCTION OF CHITOSAN FROM WASTE OF RAJUNGAN CRAB SHELL AND IT’S APLICATION IN CHOLESTEROL REDUCTION BY IN VITRO TEST</dc:title>
	<dc:creator xml:lang="en">Nasir Maidin, Alfian</dc:creator>
	<dc:creator xml:lang="en">Natsir, Hasnah</dc:creator>
	<dc:creator xml:lang="en">Dali, Seniwati</dc:creator>
	<dc:subject xml:lang="en">Rajungan</dc:subject>
	<dc:subject xml:lang="en">chitosann</dc:subject>
	<dc:subject xml:lang="en">Bacillus licheniformis HSA3-1a</dc:subject>
	<dc:subject xml:lang="en">chitin de-acetylase</dc:subject>
	<dc:subject xml:lang="en">cholesterol</dc:subject>
	<dc:description xml:lang="en">This research aimed to show the percentages of the level of the cholesterol decreased by the convertion product of chitosan enzimatically through in vitro. The research method was started by the demineralization process of the sample in the form of powder of crab shells, and then continued with the de-colorization and de-proteination steps. The next step was de-asetilation enzymatically using de-acetylated chitin which had been isolated from Bacillus licheniformis HSA3-1a bacteria at 50OC for 2 hours until the chitosan was obtained. The research results indicated that the characteristics of the isolated chitosan had water content of 5.45%, ash content 1.53%, N-total of 6.99% and de-acetylation degree of 78.9%, and it showed white yellowish powder form. The results of the test of chitosan as the reducer of cholesterol level by in vitro process showed that 31.18% of the cholesterolwas decreased by 2 mg concentrate of chitosan. Beide, 0.5 mg of simvastatin (as positive control) had decreased by 58.29%. </dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2019-05-12</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ica/article/view/6404</dc:identifier>
	<dc:identifier>10.70561/ica.v10i1.6404</dc:identifier>
	<dc:source xml:lang="en">Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta); Volume 10, No.1: June 2017; 25-34</dc:source>
	<dc:source>2655-6049</dc:source>
	<dc:source>2085-014X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ica/article/view/6404/3503</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2019 Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)</dc:rights>
</oai_dc:dc>