Melola Discovery

← Melola Discovery

Open access Jurnal

Antibacterial Activity Test of Elephant ginger (Zingiber officinale Rosc.) Endophytic Fungi Variation of Elephants Against Bacteria That Cause Skin Infections

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) · Vol. ume No. 2 (2020) · pp. 68–72

DOI
10.70561/ica.v13i2.10771
ISSN
2655-6049, 2085-014X
Published
2020-12-19
Publisher
Hasanuddin University
Language
en
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Elephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.lephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.

Keywords

Collaboration network of the authors

17 authors · 29 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Eka SuaibUniversitas Halu Oleo1
Rahman TandiAffiliation unknown1
Agussalim BurhanuddinAffiliation unknown1
Nurul Annisa RasbilAffiliation unknown1
Munif Arif RantiAffiliation unknown1
Sumarheni SudirAffiliation unknown1
Yulica TumarukAffiliation unknown1
Burhanuddin TaebeAffiliation unknown1
Fitri AmaliaAffiliation unknown1
Zaraswati DwyanaAffiliation unknown1
RusliAffiliation unknown1
SeniwatiAffiliation unknown1
Adelia FitrahAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ica/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Antibacterial Activity Test of Elephant ginger (Zingiber officinale Rosc.) Endophytic Fungi Variation of Elephants Against Bacteria That Cause Skin Infections</dc:title>
	<dc:creator xml:lang="en">seniwati, seniwati</dc:creator>
	<dc:creator xml:lang="en">Aprilia, Laras</dc:creator>
	<dc:creator xml:lang="en">Rusli, Rusli</dc:creator>
	<dc:creator xml:lang="en">Naid, Tadjuddin</dc:creator>
	<dc:subject xml:lang="en">Antibacterial</dc:subject>
	<dc:subject xml:lang="en">endophytic fungi</dc:subject>
	<dc:subject xml:lang="en">elephant ginger</dc:subject>
	<dc:subject xml:lang="en">TLC-Bioautography</dc:subject>
	<dc:subject xml:lang="en">bacteria that cause skin infections.</dc:subject>
	<dc:description xml:lang="en">Elephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7.lephant ginger rhizome has the potential as an antibacterial, therefore the research was conducted to test the antibacterial activity of elephant ginger endophytic fungi against bacteria that cause skin infections, determine test bacteria that can be inhibited by elephant ginger endophytic fungi and bioautogram profile for its antibacterial activity. Isolation of endophytic ginger elephant fungi using the direct planting method. Screening of endophytic fungi isolates was placed on the surface of NA medium containing test bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acne. The screening results obtained were 2 isolates namely isolates which had the highest activity namely IFDRJG03 and IFDRJG04. For IFDRJG03 isolates can inhibit the antibacterial activity of Staphylococcus aureus 25.26 mm, Staphylococcus epidermidis 26.89 mm and Propionibacterium acne 27.12 mm, IFDRJG04 isolates can inhibit the antibacterial activity of Staphylococcus aureus 15.87 mm, Staphylococcus epidisidis 18 and 96 mm. 27.43 mm. Then the TLC-Bioautography test was carried out, the results of IFDRJG03 isolates were obtained using chloroform eluents: methanol (1: 1) had antibacterial activity against Staphylococcus aureus with Rf1 = 0.9 and Rf2 = 0.5, Staphylococcus epidermidis with Rf1 value = 0.8 and Rf2 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.5 and isolation of IFDRJG04 isolates with ethyl acetate eluent: ethanol: water (8: 2: 1) has antibacterial activity against Staphylococcus aureus Rf1 values = 0.9 Rf2 = 0.7 Rf3 = 0.6, Staphylococcus epidermidis with Rf1 value = 0.7 Rf2 = 0.6, Rf3 = 0.5 and Propionibacterium acne with Rf1 value = 0.9 and Rf2 = 0.7. </dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2020-12-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ica/article/view/10771</dc:identifier>
	<dc:identifier>10.70561/ica.v13i2.10771</dc:identifier>
	<dc:source xml:lang="en">Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta); Volume 13, No 2: December 2020; 68-72</dc:source>
	<dc:source>2655-6049</dc:source>
	<dc:source>2085-014X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ica/article/view/10771/6160</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>