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Anti-free radical activity test of endophytic fungal fermentate extract on the Snake Plants (Sansevieria trifasciata Hort. Ex Prain) using the TLC-Autography method

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) · Vol. ume No. 2 (2021)

DOI
10.70561/ica.v14i3.14496
ISSN
2655-6049, 2085-014X
Published
2021-12-25
Publisher
Hasanuddin University
Language
en
License
creativecommons.org/licenses/by-sa/4.0

Abstract

Degenerative diseases occur due to damage to cells, fat tissue, and the immune system caused by several factors. One theory that causes degenerative diseases is the presence of free radicals. The Snake plant is one of the plants in Indonesia that has long been known as a plant that is useful as anti-free radicals. The research was conducted by isolating and purifying endophytic fungi and determining the autogram profile of free antiradical activity from The Snake plant. From the purification results obtained 7 isolates, followed by macroscopic examination and fermentation of each isolate. The results of the screening test showed that 2 fermentates were active as anti-free radicals, namely AF 1 and AF 3. Fermentate isolates AF 1 and AF 3 were identified by TLC-autography using a mixture of n-hexane: ethyl acetate (7:1). TLC-autography test results by spraying DPPH isolates AF 1 and isolates AF 3 showed yellow spots on a purple background with Rf1 values ​​of 0.90 and Rf2 of 0.2 in AF1 isolates and Rf1 values ​​of 0.83 and Rf2 of 0.16 on AF3 isolates. potential as anti-free radicals. From the results of this study, it was concluded that there was anti-free radical activity in the Snake plant.

Collaboration network of the authors

22 authors · 39 co-authorship links

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  • State University of Malang
  • Affiliation unknown
  • Different organisations
  • Same organisation
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Show as table
Co-authorOrganisationJoint articles
Boy Piter Nizu KekriAffiliation unknown1
Muhammad AsharState University of Malang1
Nurjannah AbdullahAffiliation unknown1
Andi HaminaAffiliation unknown1
DarwisAffiliation unknown1
Burhanuddin Siti NurazizahAffiliation unknown1
Munif Arif RantiAffiliation unknown1
Oktaviano Nandito GunturAffiliation unknown1
Ibnu Aly BadiuAffiliation unknown1
Sumarheni SudirAffiliation unknown1
Yulica TumarukAffiliation unknown1
Burhanuddin TaebeAffiliation unknown1
Rusli RusliAffiliation unknown1
Seniwati SeniwatiAffiliation unknown1
Laras ApriliaAffiliation unknown1
Syadza FirdausiahAffiliation unknown1
Nur MadyaAffiliation unknown1
Meidistria Tandi RapakAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ica/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Anti-free radical activity test of endophytic fungal fermentate extract on the Snake Plants (Sansevieria trifasciata Hort. Ex Prain) using the TLC-Autography method</dc:title>
	<dc:creator xml:lang="en">Seniwati</dc:creator>
	<dc:creator xml:lang="en">Rusli</dc:creator>
	<dc:creator xml:lang="en">Adelia Fitrah</dc:creator>
	<dc:creator xml:lang="en">Naid, Tadjuddin</dc:creator>
	<dc:description xml:lang="en">Degenerative diseases occur due to damage to cells, fat tissue, and the immune system caused by several factors. One theory that causes degenerative diseases is the presence of free radicals. The Snake plant is one of the plants in Indonesia that has long been known as a plant that is useful as anti-free radicals. The research was conducted by isolating and purifying endophytic fungi and determining the autogram profile of free antiradical activity from The Snake plant. From the purification results obtained 7 isolates, followed by macroscopic examination and fermentation of each isolate. The results of the screening test showed that 2 fermentates were active as anti-free radicals, namely AF 1 and AF 3. Fermentate isolates AF 1 and AF 3 were identified by TLC-autography using a mixture of n-hexane: ethyl acetate (7:1). TLC-autography test results by spraying DPPH isolates AF 1 and isolates AF 3 showed yellow spots on a purple background with Rf1 values ​​of 0.90 and Rf2 of 0.2 in AF1 isolates and Rf1 values ​​of 0.83 and Rf2 of 0.16 on AF3 isolates. potential as anti-free radicals. From the results of this study, it was concluded that there was anti-free radical activity in the Snake plant.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2021-12-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ica/article/view/14496</dc:identifier>
	<dc:identifier>10.70561/ica.v14i3.14496</dc:identifier>
	<dc:source xml:lang="en">Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta); Volume 14, No 2: December 2021 </dc:source>
	<dc:source>2655-6049</dc:source>
	<dc:source>2085-014X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ica/article/view/14496/7751</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2021 Seniwati, Rusli, Adelia Fitrah, Tadjuddin Naid</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>