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The Analysis of Total Phenolic Content of Siam Weed Leaf Extract (Chromolaena Odorata L.) and Antibacterial Activity Test Against Escherichia coli and Staphylococcus aureus

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) · Vol. ume No. 1 (2022) · pp. 29–34

DOI
10.70561/ica.v15i1.21074
ISSN
2655-6049, 2085-014X
Published
2022-11-06
Publisher
Hasanuddin University
Language
en
License
creativecommons.org/licenses/by-sa/4.0

Abstract

This study aims to determine the total phenolic content of extracts of water, methanol, and ethyl acetate of Siamese weed leaves and to test their bioactivity against Escherichia coli and Staphylococcus aureus. This research includes testing of active compound groups and determination of total phenolic and bioactivity tests. The total phenolic content was determined by the Folin-Ciocalteu method using a UV-Vis spectrophotometer. The results of the analysis showed that the extracts of water, methanol, and ethyl acetate were 54.0647; 44.9289; and 9.2005 mg GAE/g extract. The results of the bioactivity test of aqueous extracts were greater than that of methanol and ethyl acetate extracts where the minimum inhibitory concentration (MIC) of aqueous extracts against E. coli bacteria was at a concentration of 0.1%, while against S. aureus bacteria the bioactivity of methanol extracts was greater than that of aqueous extracts and ethyl acetate concentrations. minimum inhibitory (MIC) 0.5%. Keywords: Antibacterial, Siamese weed leaves, total phenolic.

Collaboration network of the authors

23 authors · 68 co-authorship links · 4 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Diponegoro University
  • Wageningen University & Research
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Neny Rasnyanti M ArasAffiliation unknown3
Fuad MuhammadDiponegoro University2
Saadatul HusnaAffiliation unknown2
Hermin Hardyanti UtamiAffiliation unknown2
Rismul Trianto SalawaliAffiliation unknown2
Sherly TowolioeAffiliation unknown2
Andi Wahyu TrifanyAffiliation unknown2
Nada Pertiwi PaprianiAffiliation unknown2
Tri Retnaningsih SoeprobowatiDiponegoro University1
Yogiswara DanurrachmanDiponegoro University1
Peter van der MaasWageningen University & Research1
Ciana Magdalena MorinAffiliation unknown1
Subian SaidiAffiliation unknown1
Dorrah AzisAffiliation unknown1
Firdaus ZentaAffiliation unknown1
Noor JalaluddinAffiliation unknown1
La DahaAffiliation unknown1
Adinda IrwanaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ica/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">The Analysis of Total Phenolic Content of Siam Weed Leaf Extract (Chromolaena Odorata L.) and Antibacterial Activity Test Against Escherichia coli and Staphylococcus aureus</dc:title>
	<dc:creator xml:lang="en">tamuddin, yusriadi</dc:creator>
	<dc:creator xml:lang="en">Lestari, Mega Fia</dc:creator>
	<dc:creator xml:lang="en">Hasri, Hasri</dc:creator>
	<dc:creator xml:lang="en">Maryono, Maryono</dc:creator>
	<dc:creator xml:lang="en">Herawati, Netti </dc:creator>
	<dc:description xml:lang="en">This study aims to determine the total phenolic content of extracts of water, methanol, and ethyl acetate of Siamese weed leaves and to test their bioactivity against Escherichia coli and Staphylococcus aureus. This research includes testing of active compound groups and determination of total phenolic and bioactivity tests. The total phenolic content was determined by the Folin-Ciocalteu method using a UV-Vis spectrophotometer. The results of the analysis showed that the extracts of water, methanol, and ethyl acetate were 54.0647; 44.9289; and 9.2005 mg GAE/g extract. The results of the bioactivity test of aqueous extracts were greater than that of methanol and ethyl acetate extracts where the minimum inhibitory concentration (MIC) of aqueous extracts against E. coli bacteria was at a concentration of 0.1%, while against S. aureus bacteria the bioactivity of methanol extracts was greater than that of aqueous extracts and ethyl acetate concentrations. minimum inhibitory (MIC) 0.5%.
Keywords: Antibacterial, Siamese weed leaves, total phenolic.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2022-11-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ica/article/view/21074</dc:identifier>
	<dc:identifier>10.70561/ica.v15i1.21074</dc:identifier>
	<dc:source xml:lang="en">Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta); Volume 15, No 1: June 2022; 29-34</dc:source>
	<dc:source>2655-6049</dc:source>
	<dc:source>2085-014X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ica/article/view/21074/8904</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 yusriadi tamuddin</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>